41 results about "Chalcone isomerase" patented technology

The invention relates to a tulip chalcone isomerase TfCHI protein and an encoding gene thereof. The protein is composed of amino acid sequences represented by the SEQ ID No.4, or the protein is composed of amino acid sequences represented by the SEQ ID No.4 which have been processed by replacing, removing, or adding one or several amino acids, and has a tulip chalcone isomerase activity. The invention also provides a nucleic acid sequence which encodes the protein mentioned above and is represented by the SEQ ID No.3. By utilizing the tulip chalcone isomerase gene provided by the invention and various screening method, substances, acceptors, inhibitors or antagonists which can interact with the chalcone isomerase TfCHI can be screened out. By utilizing the tulip chalcone isomerase gene provided by the invention, people can change the flower color through a gene engineering method, and a novel way is provided for creating new flower colors.

An isolated nucleic acid fragment encoding a chalcone isomerase is disclosed. Also of concern is the synthesis of a recombinant DNA construct encoding all or a portion of the chalcone isomerase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the chalcone isomerase in a transformed host cell.

The invention discloses a method for producing eriodictyol by reforming escherichia coli in metabolic engineering, belonging to the field of metabolic engineering. The step of transforming naringenin into the eriodictyol is successfully realized through a gene engineering technology by carrying out fusion expression on two genes, namely a flavone 3' hydroxylase gene tF3'H with a membrane binding sequence cut and a P450 reductase gene tCPR, in escherichia coli. A tyrosine ammonia lyase gene TAL from R.glutinis, 4-coumarate:coenzyme A ligase gene 4CL from P.crispum, a chalcone synthase gene CHS from P.hybrida and a chalcone isomerase gene CHI from M.sativa are simultaneously expressed to realize that naringenin is directly produced through tyrosine, so that an engineered strain for directly producing eriodictyol through tyrosine is established. According to the method, in order to increase the output of eriodictyol, an acetokinase ackA gene contained in an escherichia coli genome is removed by being knocked, an acetyl coenzyme A synthetase gene acs and an acetyl coenzyme A carboxylase gene ACC are excessively expressed, and the output of eriodictyol can finally reach 106.7 mg / L.

The present invention provides a protein having aurone synthase activity involved in the color of flowers such as snapdragons, a gene that encodes it, cDNA in particular, and its applications. This gene can give yellow color to the flowers of plants by introducing into plants deficient in chalcone isomerase and so forth and expressing.

A method for production of a dihydrochalcone, especially of phloretin, using a transgenic microorganism, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and / or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, corresponding transgenic microorganisms, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and / or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and / or a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, and host cells, containing one or more identical or different such vectors.

The present invention clones three chalcone isomerase (CHI) cDNAs for the first time from the petals of safflower safflower, and determines its nucleotide sequence and the resulting amino acid sequence. During the flower coloring process of Cymbidium thaliana, the expression of the three genes and the synthesis of anthocyanins showed an obvious correlation. In addition, the three eukaryotic expression vectors of FhCHI were transfected into the CHI mutant of Arabidopsis thaliana, and the seedlings and seed coats of the three FhCHI mutants were both The phenotype was restored.

The invention discloses eggplant chalcone isomerase SmCHI protein and a coding gene thereof. The protein comprises (a) protein consisting of an amino acid sequence as shown in SEQ ID NO.3; or (b) protein derived from the amino acid sequence in (a), which is substituted, lost or added by one or more amino acids and has the activity of eggplant chalcone isomerase. The cDNA and gDNA of the coding gene respectively have base sequences as shown in SEQ ID NO.1 and SEQ ID NO.2; and the gene is expressed in roots, stems, leaves, flowers, peels and pulp, and has the expression quantity in peels remarkably higher than that in other tissues. Under stress of low temperature, the expression quantity of the gene reaches the maximal value in 48 hours, which is 3.65 times that of an unprocessed gene. The eggplant chalcone isomerase SmCHI protein and the coding gene thereof provide a theoretical basis for improving plant quality by utilizing the genetic engineering technology to obtain high oxidation resistance medicines or foods in the future, thereby having great application values.

The invention relates to the field of biological technologies and provides a method for increasing content of artemisinin in Artemisia annua L. by genetic modification with CHI (chalcone Isomerase) genes. According to the method, the CHI genes are cloned from Artemisia annua L., a plant expression carrier containing the CHI genes is established, the CHI genes are mediated by agrobacterium tumefaciens to be transferred into Artemisia annua L. for regeneration of a plant, the integration condition of an exogenous target gene CHI is detected through the PCR (polymerase chain reaction), the content of artemisinin in transgenic Artemisia annua L. is detected through an HPLC-ELSD (high performance liquid chromatography-evaporative light scattering detector), and the transgenic Artemisia annua L. plant with the content of artemisinin increased is screened out. The content of artemisinin in transgenic Artemisia annua L. is increased and is 1.5 times that of a non-transgenic contrast plant in the highest level, and the method for increasing the content of artemisinin in Artemisia annua L. is provided and lays a foundation for large-scale production of artemisinin from transgenic Artemisia annua L..

The invention discloses a safflower chalcone isomerase (CHI) gene. The RNA (Ribonucleic Acid) of safflower pedals is reversely transcribed into a cDNA (Complementary Deoxyribonucleic Acid), the cDNA is used as a template to carry out 3' and 5' race cloning, the 3' and 5' end sequences of the safflower CHI gene are respectively obtained, a safflower CHI sequence is obtained through splicing of the 3' and 5' end sequences, the whole gene length is 1161bp, and the length of an opening reading frame is 654bp. The safflower CHI gene is named ct-(i)CHI( / i), and the result of the sequence comparison and analysis of the safflower CHI gene on ncbi (National Center for Biotechnology Information) shows that the homology of the gene with a dutchmanspipe root CHI gene reaches 82%. Experiments show that the synthesis of flavone can be promoted by the over-expression ct-(i)CHI( / i) gene, the flavone content can be lowered after the ct-(i)CHI( / i) gene is inhibited, and the safflower CHI gene has wide prospects in the aspect of cultivating high-content flavonoids transgenic plants.

The invention provides a method for increasing ginkgo biloba flavonoid content. The method includes the step of processing ginkgo biloba through genistein. Furthermore, the invention provides application of genistein in the improvement of plant flavonoid content. By applying genistein to ginkgo biloba, the physiological metabolism level in ginkgo biloba is changed, photosynthesis of ginkgo biloba is promoted, and therefore flavonoid accumulation is promoted, ginkgo biloba flavonoid content is increased, medical and economic value of ginkgo biloba is increased, operation is easy and convenient, and cost is low. According to blades processed through genistein, the content of flavonoid, anthocyanin, soluble sugar and chlorophyll is remarkably increased, activity of autioxidant enzymes, phenylalanine ammonia-lyase, chalcones synthase and chalcone isomerase is obviously enhanced, and therefore flavonoid content is increased, resistance of plants is greatly increased, yield and quality of plants are increased, and good application and popularization prospects are achieved.