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A method for increasing ginkgo flavonoid content and application of genistein

A technology of genistein and flavonoids is applied in the field of agricultural science and technology, which can solve the problems of low flavonoid content in ginkgo leaves, and achieve the effects of improving medicinal and economic value, increasing flavonoid content and promoting flavonoid accumulation.

Inactive Publication Date: 2018-03-23
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, the present invention provides a method for increasing the flavonoid content of ginkgo biloba and the application of genistein, which solves the problem of extremely low flavonoid content in ginkgo leaves, and provides a method that is easy to operate, low in cost, and can be used efficiently. Method for increasing ginkgo flavonoid content

Method used

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  • A method for increasing ginkgo flavonoid content and application of genistein
  • A method for increasing ginkgo flavonoid content and application of genistein
  • A method for increasing ginkgo flavonoid content and application of genistein

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Embodiment 1

[0021] 1 test method

[0022] The determination methods in this embodiment are as follows, and the experimental methods not specified in the implementation cases are carried out according to conventional conditions or according to the conditions suggested by the manufacturer.

[0023] 1.1 Determination of Ginkgo Chlorophyll Content

[0024] Carry out the determination of chlorophyll a, chlorophyll b content according to the following method. Take 3g of fresh leaves, punch a sample, grind, add 80% acetone to extract, measure at 663nm and 645nm wavelengths after constant volume, according to C (mg L -1 )=20.31 OD 645+8.04OD 663, (OD is spectroscopic value) to calculate the chlorophyll concentration. Finally use the formula: Y(mg dm -2 ) = [concentration (mg L -1 )×total amount of extract (mL) / [leaf area (dm 2 )×100] converted into chlorophyll content. The measurement was repeated 3 times and the average value was taken.

[0025] 1.2 Determination of anthocyanin content

...

Embodiment 2

[0074] 1 test method

[0075] The test method used in this embodiment is the same as that in Implementation 1.

[0076] 2 Experimental process and results

[0077] 2.1 Experimental process

[0078] 1) Acquisition of plant material

[0079] The test material of the present invention is a 1-year-old ginkgo seedling, and the variety is "Family Bergamot". The cultivation medium is a mixture of river sand and humus soil at a ratio of 1:2. Flower pots (diameter 25cm, height 35cm), one plant per pot, are placed in a greenhouse with 12h / d light and 60-70% relative humidity. Water each pot with 100ml of liquid fertilizer (containing 0.36g N, 0.12g P, 0.14g K) every week.

[0080] 3) Experimental treatment

[0081] When the present invention grows four leaves in the ginkgo seedlings, the GNT aqueous solution of 0 (contrast), 50, 100 and 150 μmol / L four concentration gradients is set to spray the ginkgo seedlings, and the aqueous solution contains Tween with a volume ratio of 0.2%. ...

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Abstract

The invention provides a method for increasing ginkgo biloba flavonoid content. The method includes the step of processing ginkgo biloba through genistein. Furthermore, the invention provides application of genistein in the improvement of plant flavonoid content. By applying genistein to ginkgo biloba, the physiological metabolism level in ginkgo biloba is changed, photosynthesis of ginkgo biloba is promoted, and therefore flavonoid accumulation is promoted, ginkgo biloba flavonoid content is increased, medical and economic value of ginkgo biloba is increased, operation is easy and convenient, and cost is low. According to blades processed through genistein, the content of flavonoid, anthocyanin, soluble sugar and chlorophyll is remarkably increased, activity of autioxidant enzymes, phenylalanine ammonia-lyase, chalcones synthase and chalcone isomerase is obviously enhanced, and therefore flavonoid content is increased, resistance of plants is greatly increased, yield and quality of plants are increased, and good application and popularization prospects are achieved.

Description

technical field [0001] The invention belongs to the technical field of agricultural science and technology, and in particular relates to a method for increasing the content of ginkgo flavonoids and the application of genistein. Background technique [0002] Ginkgo biloba (Ginkgo biloba L.) originates in my country, Ginkgo family Ginkgo, deciduous trees, dioecious, also known as ginkgo tree, Gongsun tree. Ginkgo biloba is the oldest relict plant in the world, known as "living fossil". As early as the 1970s, scientists have proved that Ginkgo biloba extract has a very good therapeutic effect on cardiovascular and cerebrovascular diseases. Since then, the medicinal value of Ginkgo has been widely concerned by people. Now Ginkgo has become one of the hot spots in the research and development of international medicinal plants. one. Among them, ginkgo flavonoids are the first choice natural medicine for the treatment of senile dementia, and also have very significant therapeutic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/06
CPCA01G7/06
Inventor 许锋叶家保杨湘虹张威威廖咏玲陈强文
Owner YANGTZE UNIVERSITY
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