67 results about "Phenylalanine ammonia-lyase" patented technology

The present invention is directed to phenylalanine ammonia lyase (PAL) produced by prokaryotes, wherein such prokaryotic PAL wherein the PAL variant has a greater phenylalanine-converting activity as compared to a wild-type PAL. The invention thus provides compositions of bacterial PAL and biologically active fragments, mutants, variants and analogs thereof, as well as methods for the production, purification, and use of such compositions for therapeutic and industrial purposes.

The present invention is directed to phenylalanine ammonia-lyase (PAL) produced by prokaryotes, wherein such prokaryotic PAL wherein the PAL variant has a greater phenylalanine-converting activity and / or a reduced immunogenicity as compared to a wild-type PAL. The invention thus provides compositions of bacterial PAL and biologically active fragments, mutants, variants and analogs thereof, as well as methods for the production, purification, and use of such compositions for therapeutic and industrial purposes.

The present invention is directed to phenylalanine ammonia-lyase (PAL) variants produced by prokaryotes, wherein such prokaryotic PAL variant has a greater phenylalanine-converting activity and / or a reduced immunogenicity as compared to a wild-type PAL. The invention provides compositions of prokaryotic PAL and biologically active fragments, mutants, variants or analogs thereof, as well as methods for the production, purification, and use of such compositions for therapeutic purposes, including the treatment of cancer.

Provided herein are phenylalanine ammonia-lyase (PAL) variants produced by prokaryotes, wherein such prokaryotic PAL variant has a greater phenylalanine-converting activity and / or a reduced immunogenicity as compared to a wild-type PAL. Further provided are compositions of prokaryotic PAL and biologically active fragments, mutants, variants or analogs thereof, as well as methods for the production, purification, formulation, and use of such compositions for industrial and therapeutic purposes, e.g., treating hyperphenylalaninemia, including phenylketonuria, and other disorders, including cancer.

The present invention pertains to the use of the protein phenylalanine ammonia-lyase, as well as the biologically-active derivatives of the said protein for preventing or treating diseases associated with a phenylalanine imbalance in a human or animal body. More particularly, the present invention relates to the therapeutic use of the above-cited molecules for preventing or treating a phenylalanine imbalance in vivo. This invention also deals with therapeutic compositions comprising a pharmaceutically active amount of the above-described therapeutic molecules as well as with therapeutic methods using the said therapeutic compositions. Finally, the present invention relates to processes for selecting more therapeutically-effective variants of said protein as well as to the selected variants themselves.

The present invention is directed to phenylalanine ammonia-lyase (PAL) variants produced by prokaryotes, wherein such prokaryotic PAL variant has a greater phenylalanine-converting activity and / or a reduced immunogenicity as compared to a wild-type PAL. The invention provides compositions of prokaryotic PAL and biologically active fragments, mutants, variants or analogs thereof, as well as methods for the production, purification, and use of such compositions for therapeutic purposes, including the treatment of cancer.

The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.

The present invention relates to a method for the enzymatic hydroamination of C—C double bonds catalyzed by enzymes structurally and / or functionally related to phenylalanine ammonia lyase (PAL) isolated from microorganisms of Petroselinum crispum, Rhodoturula glutinis and / or functional active derivatives thereof.

The invention relates to the technical field of flame-retardant materials, in particular to a preparation process for a high-performance environmentally-friendly PAL (Phenylalanine Ammonia-Lyase) flame-retardant material, which solves the technical problems of low reasonability and the like existing in the prior art. The process comprises the following steps of: A, separating and purifying; B, depolymerizing and dispersing; C, inorganically activating; and D, organically modifying. Compared with the prior art, the preparation process for the high-performance environmentally-friendly PAL flame-retardant material has the advantages of simple working procedure, easiness in implementation, low environmental pollution, low production cost and high product quality.

The invention provides a method for preparing germinated brown rice by utilizing a repeated mild drought stress method. The method is characterized by placing the brown rice into drought stress solution to compel the soluble sugar, free amino acids and active oxygen to be accumulated, activating protective enzymes and phenylalanine ammonia lyase and promoting the amount and activities of superoxide dismutase, hydrogen peroxidase and peroxidase to be increased and polyphenol compounds to be enriched. The method has the following advantages: the method of alternately carrying out repeated and short drought stress and high temperature germination hastening is adopted to prevent excessive active oxygen from harming the cells, thus ensuring the active oxygen to be slowly generated and not only obtaining higher contents of soluble sugars, free amino acids, superoxide dismutase, hydrogen peroxidase, peroxidase and polyphenol compounds but also improving the germination percentage of the brown rice.

The invention discloses a method for preserving sweet cherries by utilizing abscisic acid. The method comprises the following steps: soaking sweet cherries with uniform size and no mechanical damage for 15-25min through an abscisic acid solution, taking out and airing; and pouring in a PVC (polyvinyl chloride) film bag, placing for 6h at 4 DEG C under the conditions that the opening of the bag is open and the relative humidity is 78%, binding up the bag opening, and storing at 4DEG C. By adopting the method, the quality of the sweet cherries can be well maintained in the early storage period, the analysis on the activity change of catalase, peroxidase, phenylalanine ammonia-lyase and other related enzymes proves that the dehydration rate of fruits in the storage period can be obviously reduced through abscisic acid treatment, the rotting rate of the fruits can be reduced, the high commodity value and edibleness value can be kept for a long time, the capability of resisting external environment stress, especially microorganism infection of the sweet cherries is enhanced, the preservation effect is superior to that of the control group, and the storage performance of the sweet cherries can be improved, so that the method is a safe and effective simple method for preserving the sweet cherries.

The invention discloses an engineering probiotic capable of displaying phenylalanine ammonialyase on the surface, which is an escherichia coli Nissle 1917 derived bacterium, a gene argR is knocked out and / or a gene argA (Y19C) mutation is generated on a genome, and an L-phenylalanine ammonialyase gene stlA, an L-phenylalanine transport protein gene pheP, an L-amino acid deaminase gene pma and an efflux pump gene acrA are integrated. The engineering probiotics can be used for treating phenylketonuria.

The invention provides an immobilized enzyme, a preparation method therefor and application of the immobilized enzyme. The immobilized enzyme comprises activated polyethylene imine (PEI) and an enzymecovalently bound onto the activated PEI; the enzyme is selected from any one of the following enzymes: transaminase, ketoreductase, cyclohexanone monooxygenase, phenylalanine ammonia lyase, ene reductase, imine reductase, amino acid dehydrogenase and nitrilase. The enzyme and the PEI are immobilized, and the enzyme is immobilized between a poly-net structure formed by the PEI, so that the mechanical stability is improved, immobilization of the enzyme is realized, and the situation that the activity of the enzyme is reduced due to direct covalent bonding of the enzyme and cross-linking agentsincluding glutaraldehyde and the like during immobilization is avoided.

The invention relates to a method for prevention and treatment of diseases of longan fruits, wherein the method comprises the steps: (1) before picking, from the beginning of a longan young fruit stage, spraying a disease prevention and treatment liquid on longan; (2) when the maturity of the longan fruits is 80-90%, picking the longan fruits; and (3) soaking the longan fruits after picking in slightly acidic electrolyzed water, soaking for 10-15 min, then taking out, draining water, then carrying out fresh-keeping packaging on the longan fruits after draining, placing the packaged longan fruits under conditions of the temperature of 25-30 DEG C and the relative humidity of 85%-90%, and storing. The method for prevention and treatment of diseases of the longan fruits can improve the longan peel chitinase activity, beta-1, 3-glucanase activity, and the activity of phenylalanine ammonia lyase and other decrease-resistant related enzymes, and delays the decrease of the total phenolic content, so as to effectively control the occurrence of the diseases of postharvest longan fruits.

The invention discloses a lonicera japonica thunb phenylalanine ammonia-lyase (LJPAL) gene as well as a protease coded by the same and application of the gene. The LJPAL gene has nucleotide sequences shown in SEQ ID NO.1 or homologous sequences obtained through addition, substitution, insertion or deletion of one or a plurality of nucleotides or nucleotide sequences derived from alleles of the gene and the gene. A protein coded by the gene has amino acid sequences shown in SEQ ID NO.2 or homologous sequences obtained through addition, substitution, insertion or deletion of one or a plurality of amino acids.

The invention discloses an application of beta-aminobutyric acid in inducing a tea tree to generate resistance on a tea geometrid. The application comprises a method for treating the tea tree by using the beta-aminobutyric acid, a mixture of the beta-aminobutyric acid and benzothiadiazole and a mixture of the beta-aminobutyric acid and the derivative of the benzothiadiazole. By adopting the method to treat the tea tree, the activity of PPO (Polyphenol Oxidase), POD (Peroxidase) and PAL (Phenylalanine Ammonia Lyase) which are contained in a fresh tea leaf tissue is outstandingly enhanced, and the total amount of tea polyphenol, the content of catechin and the content of amino acid are increased; after fresh tea leaves treated through the method are fed, the tea geometrid is increased in developmental duration, the feeding amount and weight of larvae are outstandingly reduced, and the survival rate, the pupation rate and the eclosion rate are reduced at different degrees, so that the purpose of preventing and controlling the tea geometrid is contribute to realize.

The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.

The invention relates to the technical field of fresh bamboo shoot fresh keeping, and particularly relates to a method for prolonging the fresh-keeping period of fresh square bamboo shoots. The method specifically comprises the steps that the fresh bamboo shoots with shells are placed in a preservative solution to be soaked and then placed till no water drips, the fresh bamboo shoots are transferred into a modified atmosphere packaging bag to be sealed, and low-temperature storage is conducted. According to the fresh-keeping method, the shells do not need to be removed, the weight loss rate of the square bamboo shoots can be effectively decreased through fresh keeping with the shells, nutrient substance consumption is reduced, browning is inhibited, the situation that the surfaces of the square bamboo shoots are dewed, and microbial infection causes rot and deterioration is prevented, tissue damage after the square bamboo shoots are picked is relieved, the activity of phenylalanine ammonia lyase, cellulose synthase and other enzymes is inhibited, wood fibrosis is delayed, the respiration of bamboo shoot bodies is inhibited, and metabolism consumption of nutrient substances is reduced.

The invention relates to the technical field of flame-retardant materials, in particular to a process for depolymerizing and purifying a high-performance and environment-friendly phenylalanine ammonia lyase (PAL) flame-retardant material, and solves the technical problems that the prior art is unreasonable and the like. The process comprises the following steps of: A, depolymerizing; and B, purifying. Compared with the prior art, the process for depolymerizing and purifying the high-performance and environment-friendly PAL flame-retardant material has the advantages that: the dynamic action of hydration expansive force generated by hydration of a high-speed water medium and a substance and repulsive force of a diffused electric double layer formed by fully hydrating interlayer cations is fully exerted, disintegration and dispersion are promoted, the material is separated from impurities and purified (the medium water can be recycled), chemical impurities are prevented from being introduced and environment pollution harm is avoided in the conventional medicament floatation, high-purity micro / nano PAL material powder is prepared, and a material basis is provided for preparing the high-performance and environment-friendly flame-retardant material.

The invention relates to a method for preventing and treating brown rot of peaches. The method comprises the following steps: soaking peaches in hypotaurine solution at the concentration of 5-20 ppm for 0.5-2 h, processing and storing. The rot rate and the plaque diameter of peaches which have been soaked in the hypotaurine solution are obviously reduced, the activities of relative protective enzymes (phenylalanine ammonia-lyase and chitinase) are obviously increased, and brown rot of peaches is obviously suppressed. The method is safe and efficient, the process is simple, the method can be widely applied in storage of harvested peaches, and thus the method has broad market prospect.

The invention discloses a method for gene cloning of rhodotorula glutinis phenylalanine deaminase and high-efficiency expression method of the gene in a coliform strain, and belongs to the technical field of enzymology and enzyme engineering. Pure recombinase is obtained through gene cloning, recombinant bacterium construction, induced expression and recombinase purification, and the specific activity of the pure recombinase is 3U / mg. The method can be used for producing a great amount of phenylalanine ammonia lyase (PAL) in relatively short time, to meet industrial production demand.

The invention aims to provide an enzymatic preparation method of <15>NL-Phenylalanine. <15>N-ammonium sulfate and trans-cinnamic acid react to produce <15>NL-Phenylalanine under the catalyst of phenylalanine ammonia-lyase. The method of the invention has the advantages of the simple synthesis process, the higher output, compared with organic synthesis method and enzymatic method, achieving the easy substitution of N isotope, resulting in the product purity at 99 percent, the yield efficiency at 71 percent, the isotopic abundance at higher than 98 percent, the recovery rate of <15>N at 88 percent and being favorable for the large-scale production.

The invention discloses corn phenylalanine ammonia enzyme. The amino acid sequence of the corn phenylalanine ammonia enzyme is shown as SEQ ID NO:2, and the nucleotide sequence of the corn phenylalanine ammonia enzyme is shown as SEQ ID NO:1. The invention further discloses the application of the corn phenylalanine ammonia enzyme in synthesis of cinnamic acid through a biological method. A corn phenylalanine ammonia enzyme ZmPAL gene is constructed to an escherichia coli expression vector and converted to escherichia coli cells to carry out prokaryotic expression, and can be used for production of high-specific-activity plant phenylalnine ammonialyase. The obtained recombined corn phenylalanine ammonia enzyme can catalyze L-phenylalanine to remove amidogens to produce the cinnamic acid, provide the possibility of preparing the cinnamic acid through biosynthesis, and have high industrial application value.

The invention relates to pomegranate phenylalanine ammonia-lyase PAL as well as an expression gene and application thereof. A nucleotide sequence of the expression gene of pomegranate phenylalanine ammonia-lyase PAL is shown as SEQ ID NO. 1; the amino acid sequence of pomegranate phenylalanine ammonia-lyase PAL is shown as SEQ ID NO. 2. According to pomegranate phenylalanine ammonia-lyase PAL, theexpression gene of pomegranate phenylalanine ammonia-lyase PAL is cloned from pomegranate fruits by adopting RT-PCR (Reverse Transcription-Polymerase Chain Reaction) and RACE (Rapid Amplification ofcDNA Ends) technologies, and bioinformatics analysis is carried out; expression properties of fruits with different varieties in a development process are researched and theoretical foundations are provided for disclosing pomegranate fruit appearance quality a browning formation mechanism.

The invention discloses an application of chitosan in prevention and treatment of rice bacterial leaf streak. A chitosan preparation provided by the invention can significantly reduce the incidence of the rice bacterial leaf streak and the size of disease spots, significantly improve the activities of phenylalanine ammonia lyase, peroxidase and polyphenol oxidase in rice seedlings and further enhance the resistance against diseases of rice. The chitosan preparation is simple and easy for application in the prevention and treatment of the rice bacterial leaf streak, and the cost is lower.

The invention belongs to the field of tomato breeding, and specifically relates to a breeding method of tomatoes resistant to yellow leaf curl virus disease. According to the breeding method of the tomatoes resistant to the yellow leaf curl virus disease, the tomatoes are more susceptible to yellow leaf curl viruses through environment control, and it is found that physiological activity parameters of peroxidase (POD), phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO) increase by varying degrees in a short period of time after tomato materials at the seedling stage are infected with the yellow leaf curl viruses, infected varieties change relatively slowly, while disease-resistant varieties change relatively great, so that such characteristics can be used to quickly identify the mass tomato materials at the seedling stage and screen the disease-resistant varieties. The whole process is under controllable conditions, results are reliable, the method is simple, convenient, and easy to grasp, and the breeding cycle can be greatly shortened.

The invention discloses a method for inhibiting browning of fresh cut pakchoi. The method comprises the steps of performing selection, arrangement, fresh cutting, soaking, draining and preservation on pakchoi: soaking the fresh cut pakchoi in a 15-micrometer (1.5*10<-5>mol / l) brassinolide solution for 10min, draining the fresh cut pakchoi for 1h at 12-14 DEG C environment temperature and 40-50% relative humidity, loading the fresh cut pakchoi into trays, wrapping the fresh cut pakchoi with polyvinyl alcohol active films, and storing the packaged fresh cut pakchoi at 4 DEG C. The method can effectively inhibit a browning degree of a cut of the fresh cut pakchoi; the content of malondialdehyde is reduced; the activity of polyphenol oxidase and phenylalanine ammonia lyase is inhibited; the fresh cut pakchoi still has good appearance and an edible value on the twelfth day; the method has a certain popularization value.