Breeding method of tomatoes resistant to yellow leaf curl virus disease

A technology of yellow leaf curl virus and tomato, which is applied in the field of tomato breeding, can solve the problems of high cost, long cycle and complicated method, and achieve the effect of reducing time and cost, simple method and easy to master

Inactive Publication Date: 2019-11-19
WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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Problems solved by technology

This method is relatively complicated, and it is observed during the planting period, and the cycle is long, and after being determined as a disease-resistant variety, it has also affected the hybridization of this batch of germplasm and delayed the cultivation time; 3. Resistance gene det

Method used

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  • Breeding method of tomatoes resistant to yellow leaf curl virus disease
  • Breeding method of tomatoes resistant to yellow leaf curl virus disease
  • Breeding method of tomatoes resistant to yellow leaf curl virus disease

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Embodiment 1

[0019] (1) More than 80 copies of materials with excellent comprehensive properties were imported from home and abroad through seed collection and branch collection. The above materials were continuously selfed to obtain a variety of tomato materials with unknown disease resistance.

[0020] Through the above process, a plurality of tomato materials are obtained, wherein the tomato materials are as follows:

[0021] 711-2-35-19-3-5-1: In the spring of 2005, the tomato variety 'Num711' with mesocarp, storage and transportation, and red fruit was introduced from Nunhem in the Netherlands. After 3 generations of self-segregation and screening, 711 was selected -2-35-19 strain, in the second half of 2006, tomato yellow leaf curl virus broke out in the tomato breeding base of our institute. It is considered that there may be a gene for resistance to yellow leaf curl virus disease, and then it is screened by self-seeding for four consecutive generations, and the resistance identifi...

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Abstract

The invention belongs to the field of tomato breeding, and specifically relates to a breeding method of tomatoes resistant to yellow leaf curl virus disease. According to the breeding method of the tomatoes resistant to the yellow leaf curl virus disease, the tomatoes are more susceptible to yellow leaf curl viruses through environment control, and it is found that physiological activity parameters of peroxidase (POD), phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO) increase by varying degrees in a short period of time after tomato materials at the seedling stage are infected with the yellow leaf curl viruses, infected varieties change relatively slowly, while disease-resistant varieties change relatively great, so that such characteristics can be used to quickly identify the mass tomato materials at the seedling stage and screen the disease-resistant varieties. The whole process is under controllable conditions, results are reliable, the method is simple, convenient, and easy to grasp, and the breeding cycle can be greatly shortened.

Description

technical field [0001] The invention belongs to the field of tomato breeding, and in particular relates to a tomato breeding method resistant to yellow leaf curl virus disease. Background technique [0002] Tomato yellow leaf curl virus disease (TYLCVD) was first discovered in the Jordan River area of ​​Israel in 1939 and was officially named in 1964. According to reports, the disease has spread to more than 30 countries and regions around the world and has become a devastating disease in tomato production; in my country, the disease first appeared in 2005, first occurred in Taiwan, Guangdong and Guangxi, and then spread. to Zhejiang, Shanghai, Yunnan, Jiangsu, Henan, Fujian and Hainan; in our province, the tomatoes in Wenzhou, Jiaxing and Ningbo suffered the most. [0003] Tomato yellow leaf curl virus disease (TYLCVD) is caused by tomato yellow leaf curl virus (Tomato yellow leaf curl virus, TYLCV) of the genus Begomovirus in the family Geminiviridae and is transmitted thr...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01G22/05A01G7/06C12Q1/26C12Q1/28C12Q1/527
CPCA01G7/06A01H1/02A01H1/04A01G22/05C12Q1/26C12Q1/28C12Q1/527
Inventor 宰文珊史建磊叶曙光马彦如熊自立张海利
Owner WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
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