Pomegranate phenylalanine ammonia-lyase PAL as well as expression gene and application thereof

A technique for pomegranate phenylalanine ammonia lyase and gene expression, applied in the field of genetic engineering

Inactive Publication Date: 2018-07-24
SHANDONG INST OF POMOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on PAL gene cloning, bioinformatics and expression analysis in pomegranate fruit has not been reported at home and abroad.

Method used

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  • Pomegranate phenylalanine ammonia-lyase PAL as well as expression gene and application thereof
  • Pomegranate phenylalanine ammonia-lyase PAL as well as expression gene and application thereof
  • Pomegranate phenylalanine ammonia-lyase PAL as well as expression gene and application thereof

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Embodiment

[0032] (1) Total RNA extraction

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Abstract

The invention relates to pomegranate phenylalanine ammonia-lyase PAL as well as an expression gene and application thereof. A nucleotide sequence of the expression gene of pomegranate phenylalanine ammonia-lyase PAL is shown as SEQ ID NO. 1; the amino acid sequence of pomegranate phenylalanine ammonia-lyase PAL is shown as SEQ ID NO. 2. According to pomegranate phenylalanine ammonia-lyase PAL, theexpression gene of pomegranate phenylalanine ammonia-lyase PAL is cloned from pomegranate fruits by adopting RT-PCR (Reverse Transcription-Polymerase Chain Reaction) and RACE (Rapid Amplification ofcDNA Ends) technologies, and bioinformatics analysis is carried out; expression properties of fruits with different varieties in a development process are researched and theoretical foundations are provided for disclosing pomegranate fruit appearance quality a browning formation mechanism.

Description

technical field [0001] The invention relates to pomegranate phenylalanine ammonia-lyase PAL and its expression gene and application, belonging to the technical field of genetic engineering. Background technique [0002] The phenylpropanoid pathway is one of the unique secondary metabolic pathways of plants, which can be metabolized into lignin, flavonoids, alkaloids and other biologically active substances. Phenylalanine ammonia-lyase (PAL, E.C 4.3.1.5) is the rate-limiting enzyme in the first step of phenylpropane metabolism, which can catalyze L-phenylalanine (L-phenylalanine, L-Phe) Deamination produces cinnamic acid, which is a key bridge connecting primary metabolism and phenylpropanoid metabolism [1-2] . PAL is not only related to plant disease resistance and stress resistance [3] , also participate in the synthesis of anthocyanins and lignin, affect the appearance quality of fruit and tissue browning [4] . [0003] Pomegranate (Punica granatum L.) fruit is rich i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/60C12N9/88A01H5/00A01H6/00
CPCC12N9/88C12N15/8243C12Y403/01005
Inventor 冯立娟尹燕雷杨雪梅唐海霞
Owner SHANDONG INST OF POMOLOGY
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