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Application of chitosan in prevention and treatment of rice bacterial leaf streak

A chitosan and bacterial technology, applied in the application field of chitosan in the prevention and treatment of rice bacterial streak disease, can solve the problems of poor control effect and less pesticides

Inactive Publication Date: 2011-05-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides an application of chitosan in the prevention and treatment of rice bacterial streak disease, which solves the problems of few existing medicaments and poor control effect

Method used

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  • Application of chitosan in prevention and treatment of rice bacterial leaf streak
  • Application of chitosan in prevention and treatment of rice bacterial leaf streak
  • Application of chitosan in prevention and treatment of rice bacterial leaf streak

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 prepares chitosan solution

[0019] Dissolve chitosan powder with a deacetylation degree of 85% in 1% acetic acid by volume to prepare a chitosan solution with a concentration of 5 mg / mL, adjust the pH to 6.0 with NaOH, shake at room temperature for 24 hours, Sterilize in an autoclave at 121°C for 20 minutes and set aside. Sterile deionized water with a pH of 6.0 was used as the control of chitosan treatment in the experiment.

Embodiment 2

[0020] The impact of chitosan on the growth of Xoc BLS-01 was detected under the conditions of embodiment 2 in vitro

[0021] The strain Xoc BLS-01 in this example is provided by the Institute of Biotechnology of Zhejiang University. This strain is the test material, and its characteristics have no influence on the test results. The present embodiment adopts counting method to detect the impact of chitosan on the growth of Xoc BLS-01, and the specific method is as follows:

[0022] Xoc BLS-01 was cultured in agar medium at 28°C for 48 hours, and the bacterial suspension was prepared with sterile water (10 9 CFU / mL). 10-fold serial dilution, pipette 10 μL of Xoc bacterial suspension of each concentration onto the plate, and place the treated plate in an incubator at 28°C for 48 hours. In the experiment, sterile deionized water with a pH of 6.0 was used as the control of chitosan treatment, and six replicates were set up for each treatment. Experiments were repeated twice.

Embodiment 3

[0023] Antibacterial activity when embodiment 3 detects different concentrations of chitosan

[0024] In the chitosan solution of 5 mg / mL prepared in Example 1, add sterile deionized water to prepare a concentration of 0.05, 0.10, and 0.20 mg / mL chitosan solution. Inoculate Xoc bacteria suspension (10 8 CFU / mL), shaking culture at 160r / min, 28°C for 4 hours, collect samples to detect the number of bacteria according to Example 2. Sterile deionized water with a pH of 6.0 was used as the control of chitosan treatment in the experiment.

[0025] Such as figure 1 As shown, chitosan solution showed significant antibacterial activity against Xoc BLS-01 at different concentrations, and the antibacterial activity gradually increased with the increase of concentration. Compared with the control, the number of cells in the chitosan solution decreased by 1.88Log CFU / mL at a concentration of 0.05mg / mL, and the number of bacteria decreased by 2.05Log CFU / mL at a concentration of 0.10mg / ...

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PUM

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Abstract

The invention discloses an application of chitosan in prevention and treatment of rice bacterial leaf streak. A chitosan preparation provided by the invention can significantly reduce the incidence of the rice bacterial leaf streak and the size of disease spots, significantly improve the activities of phenylalanine ammonia lyase, peroxidase and polyphenol oxidase in rice seedlings and further enhance the resistance against diseases of rice. The chitosan preparation is simple and easy for application in the prevention and treatment of the rice bacterial leaf streak, and the cost is lower.

Description

technical field [0001] The invention relates to the field of biological control of plant protection technology, in particular to the application of chitosan in the control of rice bacterial streak disease. Background technique [0002] Thin stripe disease is also an important bacterial disease on rice. It was first reported by the Philippines in 1918 and was mistaken for rice bacterial stripe disease (Bacterail stripe of rice) for several years. Bacterial spot of rice was originally known as Xanthomonas translucents f.sp.oryzea, which was mistaken for bacterial blight. Afterwards, Goto made a comparative study of this pathogen and several varieties of the sativa-like fungus and proposed to replace Xanthomonas oryzicola with Xanthomonas translucents f.sp.oryzea. Subsequently, Bradbury (1971) revised it to Xanthomonas translucents f. sp.oryzicola. Eventually, Dye was renamed Xanthomonas camperstris pv.oryzicola. In 1990, Swing et al. re-recognized the species of Xanthomonas ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P1/00
Inventor 李斌刘宝平唐乔梅石雨陶中云谢关林
Owner ZHEJIANG UNIV
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