Increasing plant growth and yield by using a phenylalanine ammonia lyase sequence

一种核酸序列、作物产量的技术,应用在使用载体引入外来遗传物质、植物肽、生物化学设备和方法等方向,能够解决技术费力等问题

Inactive Publication Date: 2019-02-05
BENSON HILL BIOSYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such selective breeding techniques have several drawbacks, namely, that these techniques are generally laborious and produce plants that often contain heterologous genetic components, which may not always result in the desired trait being passed down from the parental plants

Method used

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  • Increasing plant growth and yield by using a phenylalanine ammonia lyase sequence
  • Increasing plant growth and yield by using a phenylalanine ammonia lyase sequence
  • Increasing plant growth and yield by using a phenylalanine ammonia lyase sequence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Example 1 - Construction of PAL Plant Transformation Vectors

[0106] An open reading frame encoding the maize PAL protein was synthesized. The open reading frame comprises SEQ ID NO:1, which encodes the protein sequence of SEQ ID NO:2. Appropriate restriction sites are included at the 5' and 3' ends of the coding sequence to permit cloning of the DNA into plant transformation vectors containing genetic elements suitable to control gene expression. In each plant transformation construct, the PAL open reading frame was located downstream of the plant promoter and 5' untranslated region (5'UTR) and upstream of the 3'UTR. Table 2 summarizes the constructed plant transformation constructs containing the PAL open reading frame.

[0107] Table 2: PAL plant transformation constructs

[0108]

[0109] In addition to the monogenic PAL plant transformation constructs listed in Table 2, multigenic plant transformation constructs containing the PAL gene cassette and the secon...

Embodiment 3

[0117] Example 3 - Transformation of Zea mays

[0118] A. tumefaciens cells carrying the PAL plant transformation vector were used to transform maize (Zea mays cv. B104) cells suitable for regeneration on tissue culture medium. After transformation of maize cells with relevant plant transformation vectors and regeneration of maize plants, PCR analysis was performed to confirm the presence of the gene of interest in the maize genome. Transformation vector number 131556 was used for maize transformation.

Embodiment 4

[0119] Example 4 - Transformation of Rice (Oryza sativa)

[0120]A. tumefaciens cells carrying the PAL plant transformation vector were used to transform rice (Oryza sativa cv. Kitaake) cells suitable for regeneration on tissue culture medium. After transforming rice cells with relevant plant transformation vectors and regenerating rice plants, PCR analysis was performed to confirm the presence of the gene of interest in the rice genome. Transformation vector number 130925 was used for rice transformation resulting in 15 PCR-validated transgenic plants containing the PAL gene cassette.

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Abstract

Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding phenylalanine ammonia lyase (PAL) proteins, polypeptides encompassing PAL proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.

Description

field of invention [0001] The present invention relates to compositions and methods for increasing plant growth and yield by expressing a phenylalanine deaminase gene in a plant. Background technique [0002] The ever-increasing world population and dwindling supply of agriculturally arable land drive the development of plants with increased biomass and yield. Conventional crop and horticultural improvements employ selective breeding techniques to identify plants with desired traits. However, such selective breeding techniques have several drawbacks, namely, that these techniques are generally laborious and produce plants that often contain heterologous genetic elements, which may not always result in the desired trait being passed down from the parental plants. Advances in molecular biology have provided means to regulate plant germplasm. Genetic engineering of plants requires the isolation and manipulation of genetic material (typically in the form of DNA or RNA) and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/82
CPCC07K14/415C12N15/8261Y02A40/146
Inventor B·N·格蕾H·D·普里斯特
Owner BENSON HILL BIOSYST
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