Lonicera japonica thunb phenylalanine ammonia-lyase (LJPAL) gene as well as product coded by same and application of gene

A technology of phenylalanine ammonia lyase and honeysuckle, applied in the field of biological and medicinal plant genetic engineering
CN103374586AInactive Publication Date: 2013-10-30INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
Publication Date
2013-10-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a lonicera japonica thunb phenylalanine ammonia-lyase (LJPAL) gene as well as a protease coded by the same and application of the gene. The LJPAL gene has nucleotide sequences shown in SEQ ID NO.1 or homologous sequences obtained through addition, substitution, insertion or deletion of one or a plurality of nucleotides or nucleotide sequences derived from alleles of the gene and the gene. A protein coded by the gene has amino acid sequences shown in SEQ ID NO.2 or homologous sequences obtained through addition, substitution, insertion or deletion of one or a plurality of amino acids.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and mainly relates to the use of a full-length cDNA library to clone the phenylalanine ammonia-lyase gene and its encoded product and its application, especially to the biosynthesis of organic acids and flavonoid enzyme genes with pharmacologically active ingredients and their encoding The product and application belong to the field of medicinal plant genetic engineering. Background technique

[0002] The formation of medicinal plant active ingredients is the product of a unique group of genes in plant secondary metabolic pathways. With the extensive and in-depth study of plant functional genomes, the study of functional genes related to secondary metabolism synthesis of medicinal plants with unique characteristics and broad application prospects has gradually become a research hotspot. It provides a theoretical basis for the biosynthetic pathway and its regulation mechanism and explains the formati...

Claims

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