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Application of ISR mechanism based disease-resistant inductive agent in preventing and treating turfgrass disease

A technology of inducer and disease resistance, applied in the application field of disease resistance inducer based on ISR mechanism in turfgrass disease control, can solve the problem of reducing the incidence rate, limited in-depth and specific research, and research reports on turfgrass disease resistance induction. There are few problems such as prolonging the control period, saving lawn maintenance costs, and enhancing the plant's own resistance.

Inactive Publication Date: 2015-06-10
马晖玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of plant ISR, some rhizosphere growth-promoting bacteria analogs can reduce the incidence of aboveground parts of plants by initiating the disease resistance mechanism in plants. The in-depth and specific research on ISR-related inducers is very limited. The research on ISR-based methods There are very few research reports on the induction of disease resistance in turfgrass

Method used

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  • Application of ISR mechanism based disease-resistant inductive agent in preventing and treating turfgrass disease
  • Application of ISR mechanism based disease-resistant inductive agent in preventing and treating turfgrass disease
  • Application of ISR mechanism based disease-resistant inductive agent in preventing and treating turfgrass disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The 2,3-butanediol stereoisomer mixture used in the present invention is purchased from West Asia Reagents, with a CAS number of 513-85-9 and a molecular formula of C 4 h 10 o 2 , the molecular weight is 90.12.

[0066] Industrial production method of 2,3-butanediol: It can be obtained by hydrolysis of four-carbon hydrocarbons produced by petroleum cracking under high temperature and high pressure. At present, the production of 2,3-butanediol by microbial fermentation is becoming its production source. For example: sugar, molasses, malt pulp or alcohol mother liquor, etc. are used as raw materials and produced by biological fermentation. When sugar is fermented to produce alcohol and then vinegar, the by-product acetaldehyde can also generate 2,3-butanediol under the action of yeast.

[0067] Inoculation of Rhizoctonia solani after the treatment of creeping bentgrass by the inducer of the present invention, the analysis of its induction effect:

[0068] Soak creepi...

Embodiment 2

[0072] Inoculation of Fusarium oxysporum after the treatment of creeping bentgrass by the inducer of the present invention, the analysis of its induction effect:

[0073] Creeping bentgrass was planted as described in Example 1. On the 8th day after planting, 150-550 μmol / L 2,3-butanediol was set up with 5 concentration gradients, and 10ml was injected into the root of creeping bentgrass, and the same volume of sterile water was injected as a control. On the 7th day after treatment with the inducer, the spore suspension of Fusarium oxysporum (concentration of 1×10 6 per ml), 7ml per bottle, and spray the same volume of sterile water as the control. After inoculation, culture in the dark at 28°C for 1 day, and then culture in a tissue culture room at 26-27°C. On the 21st day after inoculation, the effect of 250 μmol / L 2,3-butanediol stereoisomer mixture on creeping bentgrass to induce fusarium wilt was obvious, and the disease resistance effect could reach 78.26%. The inducti...

Embodiment 3

[0077] Inoculation of Rhizoctonia solani after the induction agent of the present invention handles bluegrass, the analysis of its induction effect:

[0078] Bluegrass bluegrass was planted in tissue culture bottles. On the 20th day after emergence, 150-550μmol / L 2,3-butanediol was set up with 5 concentration gradients, and 10ml was injected into the root of bluegrass bluegrass respectively. The control injection was the same volume without bacteria water. On the 7th day after the inducer treatment, the mycelia suspension of Rhizoctonia solani was spray-inoculated (concentration is OD 380 =0.8), 4ml per bottle, cultivated at 25°C, and the same volume of sterile water was sprayed as the control. On the 30th day after inoculation, the effect of 550 μmol / L 2,3-butanediol stereoisomer mixture on the induction of bluegrass blue spot was obvious, and the disease resistance effect could reach 68.5%. The induction effect was as follows: image 3 As shown, Table 3 is the suitable con...

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Abstract

The invention discloses application of an ISR mechanism based disease-resistant inductive agent in preventing and treating turfgrass disease. The inductive agent is a 2, 3-butanediol stereisomer mixture, and can induce the original herbal disease resistance of turfgrass, and has obvious preventing and treating effects on agrostis stolonifera, meadow grass, brown blotch of perennial ryegrass and festuca arundinacea and fusarium blight by applying to the root part with the most proper concentrations (250, 550, 450 and 250 mu mol / L), and the effect of resisting brown blotch can respectively reach 86, 68.5, 73.2 and 81%. When the inductive agent is affected to agrostis stolonifera, the activities of phenylalnine ammonialyase, chalcone isomerase and 4-coumarate coenzyme A ligase are all improved obviously. The inductive agent can obviously increase the content of secondary metabolism products relevant to the agrostis stolonifera resistance, such as total hydroxybenzene, flavones, lignin, can be applied to the prevention and treatment of turfgrass disease, can reduce the use of chemical bactericides, protect the environment, and reduce the turfgrass maintenance expense.

Description

technical field [0001] The invention relates to the application of a plant disease resistance inducer based on an ISR mechanism in the prevention and control of lawn diseases. Background technique [0002] Turf is an important part of urban greening. In recent years, with the improvement of people's living standards and the enhancement of environmental protection awareness, the turf industry in my country has developed rapidly. With the introduction of a large number of foreign grass species, the continuous expansion of green areas and the improvement of intensive management of lawns, turf diseases have become one of the main factors that affect the use value of turf and curb the development of today's turf industry. During the growth of turfgrass, it is infected by a variety of pathogens, causing discoloration, withering, wilting, and rot on the turf, which seriously affects the overall ornamental value and use value of the turf, and reduces the quality and useful life of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N31/02A01P21/00
Inventor 马晖玲房媛媛姚拓张锁科马源张咏梅刘兴菊马祥
Owner 马晖玲
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