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Application of PINNATE PENTAFOLIATA2 gene in regulating and controlling leaflet quantity of leguminous plants

A leguminous plant and gene technology, applied in the field of genetic engineering, can solve the problems that the production performance of multi-leaf alfalfa varieties does not show obvious advantages, and the regulation of small leaves has not yet been seen.

Inactive Publication Date: 2017-11-10
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These defects lead to the fact that the existing leafy alfalfa varieties do not show obvious advantages in terms of production performance
There is no report about the regulation of leaflet number by BEL1-LIKE gene family in compound leaf species

Method used

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  • Application of PINNATE PENTAFOLIATA2 gene in regulating and controlling leaflet quantity of leguminous plants
  • Application of PINNATE PENTAFOLIATA2 gene in regulating and controlling leaflet quantity of leguminous plants

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Example 1 Cloning of Medicago truncatula gene PINNATE PENTAFOLIATA2

[0019] 1. Cloning of Medicago truncatula PINNATE PENTAFOLIATA2 gene

[0020] Obtain the sequence of Arabidopsis thaliana PINNATE PENTAFOLIATA2 through the bioinformatics website NCBI, and then use BLAST to perform a sequence alignment search to obtain the genome sequence of PINNATE PENTAFOLIATA2 (as shown in SEQ ID No.1), and the amino acid sequence of its expressed protein is as shown in SEQ ID No.2 is shown. The primers shown in SEQ ID No.3 and SEQ ID No.4 were designed according to the sequences. The RNA of Medicago truncatula was extracted by TRIzol kit, and the full-length CDS sequence of PINNATEPENTAFOLIATA2 gene was amplified by RT-PCR. The gene sequence was connected into the pEARLEYGATE201 vector by using Gateway technology, and then the sequence was determined to verify the correctness of the cloned sequence.

[0021] Example 2 Obtaining and identification of Medicago truncatula PINNATE P...

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Abstract

The invention discloses an application of a PINNATE PENTAFOLIATA2 gene (a nucleotide sequence shown as SEQ ID NO.1) in regulating and controlling leaflet quantity of leguminous plants. According to the invention, a knockout mutant strain of the medicago truncatula PINNATE PENTAFOLIATA2 gene is obtained through separation for the first time; and it is discovered that the quantity of leaflets of plants is turned to 5 from 3, and a few of plants have 4 leaflets. Up to 97% of leaves of the obtained plants have increased leaflet quantity, and the data is much greater than existing multifoliate alfalfa varieties. It is proved that the gene participates in regulating and controlling of the leaflet quantity of leguminous plants. Transgenic leguminous plants, which are higher than wild-type plants in leaflet quantity, can be obtained by knocking out the gene, predicting that novel leguminous forage plants can be created with the implementation of the invention, and subsequent forage quality can be improved; therefore, the invention has great significance for grass industry production in China.

Description

technical field [0001] The invention relates to the application of PINNATE PENTAFOLIATA2 gene in regulating the number of leaflets of legumes, in particular to the application of knocking out PINNATE PENTAFOLIATA2 gene in increasing the number of leaves of legumes, and belongs to the technical field of genetic engineering. Background technique [0002] Leguminous forage alfalfa is the earliest cultivated, widely distributed and most important perennial high-quality forage in the world. However, the low yield, poor quality and unstable supply of domestic alfalfa have become a non-negligible factor restricting the development of grass-fed animal husbandry and dairy industry in my country. At present, the commercial production of alfalfa in my country is growing rapidly, but due to the huge domestic demand for high-quality alfalfa, the shortage of domestic alfalfa has not been alleviated. Therefore, improving the yield and quality of alfalfa is a major and urgent need for the ...

Claims

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Application Information

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IPC IPC(8): C12N15/29
CPCC07K14/415
Inventor 周传恩卢志超韩璐
Owner SHANDONG UNIV
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