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Plant stress tolerance associated protein, and coded genes and application thereof

A technology for plant stress tolerance and coding gene, which is applied in the field of plant stress tolerance-related protein and its coding gene and application field, and can solve the problem of inability to improve the salt tolerance of transgenic plants and the like

Active Publication Date: 2011-01-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to differences in species, Arabidopsis stress-tolerant genes transformed into other plants may not be able to improve the salt-tolerant ability of transgenic plants because there is no corresponding signal transduction pathway

Method used

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  • Plant stress tolerance associated protein, and coded genes and application thereof
  • Plant stress tolerance associated protein, and coded genes and application thereof
  • Plant stress tolerance associated protein, and coded genes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Cloning of embodiment 1, MtCAS31 gene

[0038] 1. Design specific primer pairs (MtCAS31_5' and MtCAS31_3'), synthesized by Invitrogen.

[0039] MtCAS31_5': 5'-TAAGATCTGGAACAGAAGTTGATTTCCGAAGAAGACCTCATGTCTCAATATCAACAAGG-3' (SEQ ID NO: 3);

[0040] MtCAS31_3': 5'-GAAGATCTCTAGTGTCCTTGTCCATGTCCAG-3' (SEQ ID NO: 4).

[0041] 2. Extract the RNA of the whole plant of truncated alfalfa A17 (Medicago truncatula), and reverse transcribe it into cDNA;

[0042] 3. Using the cDNA in step 2 as a template, use the specific primers in step 1 to perform PCR amplification on MtCAS31_5' and MtCAS31_3', and recover the PCR amplification product.

[0043] 4. Sequencing the PCR amplification product in step 3.

[0044] The sequencing result shows that the gene sequence of the PCR product is sequence 1 in the sequence table, sequence 1 is composed of 986 nucleotides, and the coding region is the 40th-978th ​​nucleotides from the 5' end of sequence 1, from The 1st-10th nucleotide at the 5'...

Embodiment 2

[0046] Example 2, Obtaining and Functional Research of Transgenic Arabidopsis

[0047] 1. Obtaining transgenic Arabidopsis

[0048] 1. Construction of recombinant vector (pCAMBIA1302-MtCAS31)

[0049] Build process like figure 1shown.

[0050] (1) The vector pMD18T-simple-MtCAS31 was single-digested with restriction endonuclease BglII, and small fragments were recovered.

[0051] (2) pCAMBIA1302 (purchased from Center for the Application of Molecular Biology to International Agriculture, www.cambia.org) was single-digested with restriction endonuclease BglII, and the vector skeleton was recovered.

[0052] (3) Ligate the small fragment of step (1) with the vector backbone of step (2) to obtain the ligation product, transform the ligation product into Escherichia coli DH5a to obtain a transformant, extract the plasmid of the transformant, and send it for sequencing, the result is The plasmid is a recombinant vector obtained by inserting sequence 1 in the sequence table betw...

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Abstract

The invention discloses a plant stress tolerance associated protein, and coded genes and application thereof. The protein provided by the invention is named MtCAS31, is from Medicago truncatula A17 and is the protein of 1) or 2): 1) the protein consisting of the amino acid sequence shown as sequence 2 in a sequence list; and 2) the protein derived from 1), associated with plant stress tolerance and formed by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence shown as sequence 2. Experiments show that: a dehydrin gene MtCAS31 selected by the invention has important value in breeding transgenic leguminous crops with salt tolerance and drought resistance.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a plant stress tolerance-related protein and its coding gene and application. Background technique [0002] Biological and abiotic factors in the environment seriously restrict the growth and development of plants, among which abiotic stresses such as high salinity, drought, low temperature, ozone, radiation and heavy metals have become the main influencing factors. 50%. Through long-term evolution, plants have formed a series of perfect adversity stress response mechanisms, including tolerance at the cellular level and coordinated responses at the overall plant level. [0003] Dehydrin is a late embryogenesis abundant protein (LEA) in plants. Exist in large quantities in the late stage of plant seed maturation. Various experiments at home and abroad have proved that dehydrin is involved in the process of plant stress resistance. Some scientists transferred the wheat dehydrin gen...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10C12N15/11A01H5/00
Inventor 董江丽王涛谢璨
Owner CHINA AGRI UNIV
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