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Application of mtunusual FLORAL ORGANS gene in regulation of leaflet number and leaf-to-stem ratio

A gene and quantity technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve problems such as no reports on regulating the number of small leaves and the ratio of leaf to stem.

Inactive Publication Date: 2020-01-07
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there is no report about the regulation of the number of small leaves and the ratio of leaf to stem by MtUNUSUAL FLORAL ORGANS gene of Medicago truncatula in compound leaf species

Method used

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  • Application of mtunusual FLORAL ORGANS gene in regulation of leaflet number and leaf-to-stem ratio
  • Application of mtunusual FLORAL ORGANS gene in regulation of leaflet number and leaf-to-stem ratio
  • Application of mtunusual FLORAL ORGANS gene in regulation of leaflet number and leaf-to-stem ratio

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0020] Example 2 Statistics on the number of leaflets of transgenic plants overexpressing Medicago truncatula MtUNUSUAL FLORAL ORGANS gene

[0021] 1. Cloning of MtUNUSUAL FLORAL ORGANS gene from Medicago truncatula

[0022] The genome sequence of MtUNUSUAL FLORAL ORGANS was obtained through the bioinformatics website NCBI, as shown in SEQ ID No.1, and its expressed amino acid sequence is shown in SEQ ID No.2. The primers shown in SEQ ID No.3 and SEQ ID No.4 were designed according to the sequences. The RNA of Medicago truncatula was extracted by TRIzol kit, and the full-length CDS sequence of MtUNUSUALFLORAL ORGANS gene was amplified by RT-PCR. The gene sequence was connected into the pEARLEYGATE201 vector by using Gateway technology, and then the sequence was determined to verify the correctness of the cloned sequence.

[0023] 2. Phenotype analysis of transgenic plants overexpressing Medicago truncatula MtUNUSUAL FLORAL ORGANS gene

[0024] Medicago truncatula was transf...

Embodiment 3

[0025] Example 3 Plant type analysis of transgenic plants overexpressing Medicago truncatula MtUNUSUAL FLORAL ORGANS gene

[0026] The phenotype of the transgenic plants overexpressing the Medicago truncatula MtUNUSUAL FLORAL ORGANS gene was analyzed, and the results showed that the leaf-stem ratio of the overexpressing plants increased by about 30% compared with the wild type ( image 3 A), the leaf area of ​​different internodes is also improved to varying degrees compared with the wild type ( image 3 B). At the same time, the plant height decreased slightly ( image 3 C-D).

Embodiment 4

[0027] Example 4 Forage quality analysis of transgenic plants overexpressing Medicago truncatula MtUNUSUAL FLORAL ORGANS gene

[0028] The forage quality of the transgenic plants overexpressing the MtUNUSUAL FLORAL ORGANS gene of Medicago truncatula was analyzed, and the results showed that the calcium, phosphorus and fat contents of the overexpressing plants were significantly higher than those of the wild type, and the actual dry matter digestibility in vitro was higher than that of the wild type. The type is also significantly improved, as shown in Table 1.

[0029] Table 1 is the determination of the forage index of the plants overexpressing the MtUNUSUAL FLORAL ORGANS gene of Medicago truncatula. The wild-type and overexpression plants with the same growth state were selected, and various indicators in the wild-type and overexpression plants were measured by near-infrared spectrum analysis. The results showed that the calcium, phosphorus and fat contents of the overexpre...

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Abstract

The invention discloses application of an MtUNUSUAL FLORAL ORGANS gene to the regulation and the control on the leaflet quantity and the leaf-stem ratio of a leguminous plant. According to the application, a knockout mutant strain of the MtUNUSUAL FLORAL ORGANS gene in medicago truncatula is separated and obtained for the first time; proven through an experiment, the gene participates in the regulation and the control on the leaflet number and the leaf-stem ratio of the leguminous plant; a transgenic leguminous plant whose leaflet number and leaf-stem ratio are higher than those of a wild type plant is obtained through the way of over-expressing the gene; the MtUNUSUAL FLORAL ORGANS gene is over-expressed in the medicago truncatula of a compound-leaf species for the first time; the quantity of leaflets of 95 percent of compound leaves can be increased; moreover, the leaf-stem ratio can be increased by approximately 30 percent. The gene is proven to participate in the regulation and the control on the leaflet quantity and the leaf-stem ratio of the leguminous plant; the transgenic leguminous plant whose leaflet number and leaf-stem ratio are higher than those of the wild type plant can be obtained through over-expressing the gene; indicatively, after being implemented, the application is about to be used for creating a novel leguminous forage plant, can be used for subsequent forage quality, and has important significance to Chinese prataculture production.

Description

technical field [0001] The present invention relates to the application of the MtUNUSUAL FLORAL ORGANS gene in regulating the number of leaflets and the leaf-to-stem ratio of legumes, in particular to the application of overexpressing the MtUNUSUAL FLORAL ORGANS gene in increasing the number of leaflets and the ratio of leaf to stem of legumes, and belongs to the technical field of genetic engineering . Background technique [0002] Leguminous forage alfalfa is the earliest cultivated, widely distributed and most important perennial high-quality forage in the world. However, the low yield, poor quality and unstable supply of domestic alfalfa have become a non-negligible factor restricting the development of grass-fed animal husbandry and dairy industry in my country. At present, the commercial production of alfalfa in my country is growing rapidly, but due to the huge domestic demand for high-quality alfalfa, the shortage of domestic alfalfa has not been alleviated. Theref...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/54
CPCC07K14/415C12N15/8261
Inventor 周传恩王小山张娟娟韩璐
Owner SHANDONG UNIV
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