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Application of MtFRUITFULLc gene in regulation and control of leaf yield and leaf protein content of leguminous plants

A legume plant, protein content technology, applied in the field of genetic engineering, can solve problems such as freezing damage, low frequency of leaflet increase, and unstable inheritance of traits

Inactive Publication Date: 2020-04-03
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the process of breeding new alfalfa varieties, most attention has been paid to the improvement of the number of small leaves of alfalfa. So far, only a few alfalfa varieties with increased number of small leaves have been commercialized, but the frequency of increased number of small leaves in these varieties is low, and the traits are not stable. Phenomena such as genetics, resulting in no obvious advantages in production
In addition, after the pasture enters the flowering period, the yield and quality of the pasture will decrease, resulting in the limitation of natural conditions such as soil stress, freezing damage, and high temperature.

Method used

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  • Application of MtFRUITFULLc gene in regulation and control of leaf yield and leaf protein content of leguminous plants
  • Application of MtFRUITFULLc gene in regulation and control of leaf yield and leaf protein content of leguminous plants
  • Application of MtFRUITFULLc gene in regulation and control of leaf yield and leaf protein content of leguminous plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Obtaining and identification of Medicago truncatula MtFRUITFULLc gene mutant

[0024] Using Thermal asymmetric interlaced-PCR (TAIL-PCR) technology, the Tnt1-marked mutant library of Medicago truncatula was screened, and the mutant line NF10716 in which Tnt1 was inserted into the MtFRUITFULLc gene was screened out of 22,000 mutant lines. The results of molecular biology identification showed that Tnt1 was inserted into the second exon of the MtFRUITFULLc gene ( figure 1 ). The MtFRUITFULLc mutant of Medicago truncatula did not flower during the reproductive period and continued to produce new leaves ( figure 2 ).

Embodiment 2

[0025] The biomass determination of embodiment 2 Medicago truncatula MtFRUITFULLc gene mutant plant

[0026] The phenotypic analysis and biomass determination of the above-ground parts of the MtFRUITFULLc gene mutant plants of Medicago truncatula showed that the leaves of the mutant plants were significantly more than those of the wild type at the same growth period after entering the reproductive period, and compared with the wild type. , and the plant height of the mutant increased by about 20% ( image 3 ), the fresh weight of the aerial parts increased by about 66% (n=10) ( Figure 4 ).

Embodiment 3

[0027] Example 3 Leaf Protein Content Determination of Medicago truncatula MtFRUITFULLc Gene Mutant Plant

[0028] The leaf protein content of the MtFRUITFULLc gene mutant plants of Medicago truncatula was measured, and the results showed that at the same growth period after entering the reproductive period, the leaf protein content of the mutant plants increased by about 18% compared with the wild type (R 2 =0.9944)( Figure 5 ).

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Abstract

The invention discloses an application of an MtFRUITFULLc gene in regulation and control of leguminous plant leaf yield and / or leaf protein content and / or plant height and / or flowering. The nucleotidesequence of the MtFRUITFULLc gene is as shown in SEQ ID No. 1. According to the invention, by using a mutant library marked by medicago truncatula Tnt1, a mutant with medicago truncatula MtFRUITFULLcgene knocked out is screened. Analysis shows that the MtFRUITFULLc gene is knocked out from medicago truncatula, so that the plant height, the fresh weight of the overground part and the content of leaf protein can be increased in the reproductive period. It is indicated that: after being implemented, the application can create novel leguminous forage plants, can be used for subsequent forage quality research, and has great significance for grassland production in China.

Description

technical field [0001] The invention relates to the application of MtFRUITFULLc gene in regulating the leaf yield and protein content of leguminous plants, and belongs to the technical field of genetic engineering. Background technique [0002] Alfalfa is an important leguminous forage, and it is also the most widely cultivated perennial high-quality forage in the world today. With the rapid growth of my country's economic level, people's demand for dairy products has increased unprecedentedly, which has promoted the rapid development of my country's grass-fed animal husbandry and dairy industry, which has also led to the increasing domestic demand for high-quality alfalfa. However, the current supply of domestic alfalfa is in short supply, and the industrial development situation is severe, with problems such as low output, poor quality, and unstable supply. Therefore, improving the yield and quality of alfalfa is a major and urgent need for the development of grass indust...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/12A01H6/54
CPCC07K14/415C12N15/8218C12N15/8251C12N15/8261C12N15/827
Inventor 周传恩张娟娟韩璐
Owner SHANDONG UNIV
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