Bacillus amyloliquefaciens cd5bc and application thereof

A technology for dissolving starch spores and bacilli, applied in the directions of application, bacteria, fungicides, etc., can solve the problems of too large difference in control effect, unsatisfactory, unfavorable promotion and use, etc., and achieve significant control effect and excellent disease resistance effect. Effect

Active Publication Date: 2016-08-31
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, although there are sporadic reports of bacterial strains that can prevent and treat certain crop diseases, such as "The biochemical mechanism of endophytic bacillus amyloliquefaciens TB2 strain active substance inducing pepper fruit resistance to blight", its control effect is not satisfactory, And it also needs to carry out the complicated extraction operation of the active substance
In terms of research on strains that can efficiently prevent and control multiple crop diseases at the same time, the existing technology has not yet achieved satisfactory results, mainly because the control effects on various crops are too different, which is not conducive to popularization and use

Method used

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  • Bacillus amyloliquefaciens cd5bc and application thereof
  • Bacillus amyloliquefaciens cd5bc and application thereof
  • Bacillus amyloliquefaciens cd5bc and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Morphological, culture characteristics of bacterial strain bacillus amyloliquefaciens cd5bc (hereinafter referred to as cd5bc) of the present invention:

[0027] cd5bc showed pale yellow colonies on NA base, the colonies were flat, round or nearly round, with shiny infiltration and irregular edges. Gram staining is positive, rod-shaped, blunt at both ends, with spores, the cysts are not enlarged, and the spores are oval ( figure 1 ). According to the morphological characteristics, it was preliminarily judged that the strain cd5bc belonged to the genus Bacillus sp. Microscopic examination, such as figure 2 .

Embodiment 2

[0029] 16S rDNA sequence analysis:

[0030] After sequencing and splicing, the 16S rDNA sequence amplified by strain cd5bc was 1466bp in length. The 16S rDNA sequence of strain cd5bc was compared with the 16S rDNA sequence of known strains in the gene bank through BLASTN on the NCBI website, and the sequence was downloaded , using MEGA to build a phylogenetic tree. The results showed that the closest genetic relationship with biocontrol bacteria cd5bc and Bacillus amyloliquefaciens. According to the morphological characteristics and phylogenetic analysis results of 16S rDNA sequence, cd5bc was identified as Bacillus amyloliquefaciens.

Embodiment 3

[0032] cd5bc biological control effect detection

[0033] 1. Plate antibacterial effect

[0034] The pathogenic bacteria Phytophthora capsici, Sclerotinia sclerotiorum and bitter gourd blight were inoculated on the PDA plate and cultured for 5 days, and the fungus cake was punched from the edge of the colony with a puncher with a diameter of 5mm, placed in the center of a blank PDA plate, and distributed equidistantly. Insert the fermented liquid of biocontrol strains on both sides (marked line), and incubate at a constant temperature of 26.5°C. When the mycelium of the pathogenic bacteria in the control dish is about to cover the whole dish, measure the growth radius of the bacteria and calculate the inhibition rate. The test was repeated 4 times. The antibacterial rate was calculated according to the size of the inhibition zone.

[0035] The results are shown in Table 1.

[0036] The biocontrol effect of table 1cd5bc on different pathogenic bacteria

[0037]

[0038] ...

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Abstract

The invention provides bacillus amyloliquefaciens cd5bc. The strain is classified and named as Bacillus amyloliquefaciens cd5bc and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO:M 2016232, and the preservation time is April 28, 2016. The invention further provides application of the strain in the aspect of preventing and controlling crop diseases and a biocontrol preparation prepared from the strain. The strain has a very significant preventing and controlling effect on red pepper phytophthora blight, the biocontrol effect reaches up to 100% after inoculation is conducted for 15 days, and the biocontrol effect reaches up to 82.36% after inoculation is conducted for 60 days. The bacillus amyloliquefaciens cd5bc has excellent disease-resistant effects on multiple crop diseases, the bacteriostasis rates of the strain on pathogenic bacteria of red pepper sclerotinia blight, bitter gourd wilt, cucumber wilt, cucumber scabs, rice blast, a rice sheath blight disease, broad bean chocolate spots and broad bean brown spots all reach up to 70% or above, and the bacteriostasis rate of the strain on the broad bean chocolate spots and broad bean brown spots reaches up to 100%.

Description

technical field [0001] The invention belongs to the field of biological control, and relates to a strain of Bacillus amyloliquefaciens cd5bc and its application, in particular to a strain of Bacillus amyloliquefaciens which has a significant control effect on pepper blight and also has high-efficiency control effects on various crop diseases. The obtained biocontrol agent and its application. Background technique [0002] In recent years, the disadvantages of using chemical agents to prevent and control crop diseases have gradually emerged, mainly manifested as damage to soil quality, soil pollution, and pesticide residues. In this context, biological control technology, especially the technology of using microorganisms to control crop diseases, has been paid more and more attention. [0003] However, the microorganisms currently used to control crop diseases are faced with too few and low effects, which limits their practical application. [0004] Pepper blight, pepper sc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 席亚东韩帅张河庆吴婕李洪浩
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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