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Bio-control bacterium foliage fertilizer, and preparation method and application thereof

A technology of bio-control bacteria and foliar fertilizer, applied in the direction of application, fertilization device, fertilizer mixture, etc., to achieve the effect of increasing yield, improving ability and long survival time

Active Publication Date: 2013-10-09
HUNAN TAIGU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no precedent for the research on compounding with Trichoderma harzianum

Method used

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  • Bio-control bacterium foliage fertilizer, and preparation method and application thereof
  • Bio-control bacterium foliage fertilizer, and preparation method and application thereof
  • Bio-control bacterium foliage fertilizer, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Composition of biocontrol foliar fertilizer: 2 parts of biocontrol, 60 parts of potassium fulvic acid, 30 parts of nano gas phase SiO2, 4 parts of active enzyme (protein powder: active enzyme mass ratio 200:1), surface activity 4 doses. The protein powder used contains protein % ≥ 50, moisture % ≤ 9.0, fat % ≤ 2.0, fineness 100 mesh sieve ≤ 1%;

[0049] Wherein, the preparation of Bacillus subtilis spore powder:

[0050] ①Slant culture: Inoculate the original strain of Bacillus subtilis on the slant medium under aseptic conditions, and cultivate it at 30°C for 48 hours; the formula of the slant medium is as follows: glucose 15g, fish peptone 5g, yeast extract 5g, water 1000mL, agar 15g.

[0051] ② Shaking table culture: Inoculate the bacteria cultured in step ① into the liquid medium under aseptic conditions, and culture in a shaking table at 160r / min for 36 hours at pH 6.5 and temperature 30°C; the formula of the liquid medium is as follows: corn Powder 13g, glucose...

Embodiment 2

[0064] Compared with Example 1, the difference is that the composition of the present embodiment biocontrol fungi foliage fertilizer is:

[0065] 1 part of bioantibiotics, 37 parts of potassium fulvic acid, nano-SiO 2 50 parts, 6 parts of enzyme active substance (protein powder: active enzyme mass ratio 200:1 mixed) and 6 parts of surfactant.

[0066] Wherein, the preparation of Bacillus subtilis spore powder:

[0067] ① Slant culture: Inoculate the original strain of Bacillus subtilis on the slant medium under sterile conditions, and culture at 29°C for 48 hours;

[0068] ② Shaking table culture: Inoculate the bacteria cultured in the above step ① into the liquid medium under sterile conditions, and culture in a shaking table at 160r / min for 36 hours at pH 6.5 and temperature 29°C;

[0069] ③Fermentation tank culture: inoculate the bacteria cultured in the above step ② into the fermenter culture medium under sterile conditions, and cultivate for 56 hours under the condition...

Embodiment 3

[0082] Compared with Example 1, the difference is that the composition of the present embodiment biocontrol fungi foliage fertilizer is:

[0083] 3 parts of bioantibiotics, 70 parts of potassium fulvic acid, nano-SiO 2 17 parts, 5 parts of enzyme active substance (protein powder: active enzyme mass ratio 200:1 mixed) and 5 parts of surfactant.

[0084] Wherein, the above-mentioned core bioantibiotics are prepared by the following steps:

[0085] Preparation of Bacillus subtilis spore powder:

[0086] ① Slant culture: inoculate the original strain of Bacillus subtilis on the slant medium under sterile conditions, and culture at 30°C for 48 hours;

[0087] ②Shaking table culture: inoculate the bacteria cultured in the above step ① into the liquid medium under sterile conditions, and culture in a shaking table at 160r / min for 48 hours at pH 6.5 and temperature 29°C;

[0088] ③Fermentation tank culture: inoculate the bacteria cultured in the above step ② into the fermenter cult...

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Abstract

The invention provides a bio-control bacterium foliage fertilizer, and preparation and application thereof. The bio-control bacterium foliage fertilizer is prepared from the following materials in parts by weight: 1-3 parts of core bio-control bacteria, 35-85 parts of biochemical fulvic acid potassium, 10-50 parts of active SiO2, 2-6 parts of an enzyme activity substance, and 2-6 parts of a plant surfactant. The core bio-control bacteria contain bio-control promoting bacteria and protein decomposing bacteria; the bio-control promoting bacteria comprise bacillus subtilis and trichoderma harzianum. The core bacteria in the bio-control bacteria foliage fertilizer can rapidly grow on the surface of a plant in a field planting manner and form a 'bio-control membrane' so as to prevent infection of pathogenic bacteria at the surface of the plant; the substances, such as antibiotics and phytohormone, are generated in the processes of interaction with the pathogenic bacteria and hyperparasitism, and a plurality of diseases of crops can be prevented, resisted or directly killed; the bio-control bacteria foliage fertilizer enables the crops to have solid plant cell wall tissue, so that the ability of plant epidermis for directly resisting incursion of the pathogenic bacteria and insect pests is enhanced, and healthy growth of the crops is facilitated; the target of using a little of pesticide or not is achieved, and the environmental pollution is alleviated. Thus, the yield and the quality of the crops are improved.

Description

technical field [0001] The invention belongs to the field of fertilizers, and in particular relates to a fertilizer containing biocontrol agents and a preparation method and application thereof. Background technique [0002] Rice blast fungus [Magnaporthe grisea (Hebert) Barr], whose asexual form is [Pyricularia grisea (Cook) Sacc], is a fungus of the genus Pyricularia oryzae, and the rice blast caused by it is one of the three major diseases of rice in my country. According to reports, the annual loss of rice production due to rice blast accounts for about 10%-15% of the total production in the world, and the economic loss reaches billions of dollars. In my country, rice blast occurs to varying degrees every year. Especially in recent years, it has continued to occur in rice-growing areas such as the southwest, the middle reaches of the Yangtze River, and the northeast. , has brought huge hidden dangers to the safe production of rice in our country. At present, the control...

Claims

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Application Information

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IPC IPC(8): C05G3/00
Inventor 唐八生曹典军陈伟彭群朱丽苏丰菲蔡浩
Owner HUNAN TAIGU BIOTECH
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