Antibiological inoculant for controlling apple tree canker, and application of antibiological inoculant
A technology of apple tree rot disease and biocontrol agent, which is applied in the application, biocide, fungicide and other directions, can solve the problem of incomplete disease prevention effect, inability to cure apple tree rot disease, and unsatisfactory Streptomyces disease prevention effect, etc. To achieve the effect of improving biocontrol effect, good strain vitality, and maintaining bacteriostatic effect
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Embodiment 1
[0065] A biocontrol agent for apple tree rot disease, mainly prepared from the following raw materials in parts by weight: 57 parts of Streptomyces albus spore powder, 40 parts of carrier, 2 parts of UV protection agent, and 1.0 part of dispersant;
[0066] The preparation method of the former powder of the spores of Streptomyces albicans comprises the following steps:
[0067] 1) Preparation of fermented seed liquid: inoculate Streptomyces albus strains on the slant of Gao's No. 1 agar medium by streaking, culture at 30°C for 7 days until sporulation; then add 5mL sterile water to the slant, inoculate with sterilized The spores were scraped off with a needle, added to sterile water, shaken and mixed, and the spore concentration was adjusted to 1×10 6 Individual / mL to obtain spore suspension; inoculate the spore suspension into the liquid seed medium according to the inoculum size 1% (v / v), 30°C, 200r / min, and shake the flask for 48h to obtain the fermented seed liquid;
[0068...
Embodiment 2
[0080] A biocontrol agent for apple tree rot disease, mainly prepared from the following raw materials in parts by weight: 55 parts of Streptomyces albus spore powder, 38 parts of carrier, 1.5 parts of UV protection agent, and 0.9 parts of dispersant;
[0081] The preparation method of the former powder of the spores of Streptomyces albicans comprises the following steps:
[0082] 1) Preparation of fermented seed liquid: inoculate Streptomyces albus strains on the slant of Gao's No. 1 agar medium by streaking, culture at 28°C for 6 days until sporulation; then add 5mL sterile water to the slant and inoculate with sterilized The spores were scraped off with a needle, added to sterile water, shaken and mixed, and the spore concentration was adjusted to 1.5×10 6 Individual / mL to obtain a spore suspension; inoculate the spore suspension into a liquid seed medium at 1% (v / v) of the inoculation amount, 28° C., 180 r / min, and shake the flask for 46 hours to obtain a fermented seed li...
Embodiment 3
[0093] A biocontrol agent for apple tree rot disease, mainly prepared from the following raw materials in parts by weight: 59 parts of Streptomyces albus spore powder, 42 parts of carrier, 2.5 parts of UV protection agent, and 1.1 parts of dispersant;
[0094] The preparation method of the former powder of the spores of Streptomyces albicans comprises the following steps:
[0095] 1) Preparation of fermented seed liquid: Inoculate Streptomyces albus strains on the slant of Gao's No. 1 agar medium by streaking, culture at 32°C for 8 days until sporulation; then add 5 mL of sterile water to the slant, and inoculate with sterile The spores were scraped off with a needle, added to sterile water, shaken and mixed, and the spore concentration was adjusted to 0.5×10 6 Individual / mL to obtain a spore suspension; inoculate the spore suspension into a liquid seed medium according to the inoculum size 1% (v / v), 32° C., 220 r / min, and shake the flask for 50 hours to obtain a fermented see...
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