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Antibiological inoculant for controlling apple tree canker, and application of antibiological inoculant

A technology of apple tree rot disease and biocontrol agent, which is applied in the application, biocide, fungicide and other directions, can solve the problem of incomplete disease prevention effect, inability to cure apple tree rot disease, and unsatisfactory Streptomyces disease prevention effect, etc. To achieve the effect of improving biocontrol effect, good strain vitality, and maintaining bacteriostatic effect

Active Publication Date: 2017-07-14
陕西枫丹百丽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The disease prevention effect of Streptomyces disclosed above is still not very satisfactory, and the disease prevention mechanism is the same as most bacteria and fungi, mainly through the metabolism to produce antibiotics, antimicrobial peptides and other antibacterial substances to produce antagonistic effects to inhibit the growth and reproduction of pathogenic bacteria, and the disease prevention effect Incomplete, can not cure apple tree rot

Method used

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  • Antibiological inoculant for controlling apple tree canker, and application of antibiological inoculant
  • Antibiological inoculant for controlling apple tree canker, and application of antibiological inoculant
  • Antibiological inoculant for controlling apple tree canker, and application of antibiological inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] A biocontrol agent for apple tree rot disease, mainly prepared from the following raw materials in parts by weight: 57 parts of Streptomyces albus spore powder, 40 parts of carrier, 2 parts of UV protection agent, and 1.0 part of dispersant;

[0066] The preparation method of the former powder of the spores of Streptomyces albicans comprises the following steps:

[0067] 1) Preparation of fermented seed liquid: inoculate Streptomyces albus strains on the slant of Gao's No. 1 agar medium by streaking, culture at 30°C for 7 days until sporulation; then add 5mL sterile water to the slant, inoculate with sterilized The spores were scraped off with a needle, added to sterile water, shaken and mixed, and the spore concentration was adjusted to 1×10 6 Individual / mL to obtain spore suspension; inoculate the spore suspension into the liquid seed medium according to the inoculum size 1% (v / v), 30°C, 200r / min, and shake the flask for 48h to obtain the fermented seed liquid;

[0068...

Embodiment 2

[0080] A biocontrol agent for apple tree rot disease, mainly prepared from the following raw materials in parts by weight: 55 parts of Streptomyces albus spore powder, 38 parts of carrier, 1.5 parts of UV protection agent, and 0.9 parts of dispersant;

[0081] The preparation method of the former powder of the spores of Streptomyces albicans comprises the following steps:

[0082] 1) Preparation of fermented seed liquid: inoculate Streptomyces albus strains on the slant of Gao's No. 1 agar medium by streaking, culture at 28°C for 6 days until sporulation; then add 5mL sterile water to the slant and inoculate with sterilized The spores were scraped off with a needle, added to sterile water, shaken and mixed, and the spore concentration was adjusted to 1.5×10 6 Individual / mL to obtain a spore suspension; inoculate the spore suspension into a liquid seed medium at 1% (v / v) of the inoculation amount, 28° C., 180 r / min, and shake the flask for 46 hours to obtain a fermented seed li...

Embodiment 3

[0093] A biocontrol agent for apple tree rot disease, mainly prepared from the following raw materials in parts by weight: 59 parts of Streptomyces albus spore powder, 42 parts of carrier, 2.5 parts of UV protection agent, and 1.1 parts of dispersant;

[0094] The preparation method of the former powder of the spores of Streptomyces albicans comprises the following steps:

[0095] 1) Preparation of fermented seed liquid: Inoculate Streptomyces albus strains on the slant of Gao's No. 1 agar medium by streaking, culture at 32°C for 8 days until sporulation; then add 5 mL of sterile water to the slant, and inoculate with sterile The spores were scraped off with a needle, added to sterile water, shaken and mixed, and the spore concentration was adjusted to 0.5×10 6 Individual / mL to obtain a spore suspension; inoculate the spore suspension into a liquid seed medium according to the inoculum size 1% (v / v), 32° C., 220 r / min, and shake the flask for 50 hours to obtain a fermented see...

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Abstract

The invention discloses an antibiological inoculant for controlling apple tree canker, and application of the antibiological inoculant. The antibiological inoculant can grow and propagate directly by using pathogenic mycelium as nutrition, and can induce the self to produce multiple extracellular enzyme capable of degrading cell walls of pathogenic bacteria; a streptomyces albidoflavus Actin-1 strain, colonized at disease spots of the apple tree canker for a long time, is activated and subjected to spore production, so that spore suspension is prepared; after that, a fermented seed solution is prepared by continuously carrying out liquid fermentation; finally, the fermented seed solution is inoculated into a solid fermentation medium, and is then fermented, dried in the air, crushed and screened so as to be prepared into spore raw powder; the spore raw powder is then scientifically screened and compounded by using auxiliary agents such as an ultraviolet protective agent, a dispersing agent and a carrier. The antibiological inoculant provided by the invention has the multiple advantages of being good in control effect, high in efficiency, low in recurrence rate and high in environmental adaptability and stability, not easy in producing drug-resistance, and the like, thus being a first choice for biological control; the antibiological inoculant has a great significance for improving the preventing and treating effect of fruit and vegetable pathogenic bacteria such as the apple tree canker, preventing pathogen recurrence and protecting environment.

Description

technical field [0001] The invention relates to a biocontrol bacterium, in particular to a biocontrol bacterium for apple tree rot disease and an application thereof. Background technique [0002] Apple tree rot (Valsa mali Miyabe et Yamada), commonly known as rotten skin disease and stinky skin disease, is an important disease of northern apple trees caused by the genus Valsa ceratosperma. The disease mainly harms fruiting trees that are more than 3 years old, causing tree vigor to weaken, branches to die, dead trees and even garden destruction. The symptoms of the disease are mainly ulcer-based. In the ulcer type, reddish-brown, water-stained, slightly raised, round to oblong lesions appear on the trunk and bark in early spring. The texture is soft, easy to tear, and easy to sag when pressed by hand. In the later stage, it shrinks and sinks, and there are cracks on the edge of the diseased part. There are obvious small black spots, that is, pycnidia. When wet, orange-yel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N63/00A01P3/00
CPCA01N63/00A01N63/10A01N2300/00
Inventor 李玲娣孙振娜王灵敏严立恩李学辉王江王楠
Owner 陕西枫丹百丽生物科技有限公司
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