Bacillus subtilis and application thereof

A technology of Bacillus subtilis and bacterial liquid, applied in the field of microorganisms, can solve the problems of hindering the application of microbial inoculants, single control effect, unstable control effect, etc. Effect

Active Publication Date: 2018-05-18
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are researches on peanut biocontrol bacteria. However, in the application process of the above three kinds of peanut pathogenic bacteria biocontrol agents, the

Method used

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Isolation, cultivation and identification of embodiment 1 bacterial strain

[0026] 1. Screening and isolation of strains

[0027] Pick a batch of single colonies on the plate, draw a line on the LB solid medium plate and place it upside down in a constant temperature incubator at 28°C for 1-2 days.

[0028] The soil was obtained from the peanut rhizosphere soil in the experimental field of Peanut Research Institute of Shandong Province. Weigh 5g of rhizosphere soil into a triangular flask of 45ml of sterile water, shake it for a while, draw 0.5ml of the supernatant and add it to a test tube containing 4.5ml of sterile water. 2 、10 3 、10 4 、10 5 、10 6 The multiples were serially diluted.

[0029] Use a micropipette to pipette 100 μl of soil suspension with different dilution factors on the NA plate, spread it evenly with a sterilized applicator, seal the plate and place it upside down in a constant temperature incubator at 28°C for 48 hours.

[0030] Pick a batch...

Embodiment 2

[0059] The mensuration of embodiment 2 bacterial strain antifungal ability

[0060] Use tweezers to inoculate small square pieces of about 0.5cm*0.5cm into PDA medium for peanut crown rot, white silkworm and peanut root rot, and inoculate them upside down in a greenhouse at 28°C for 2-3 days .

[0061] The single colony LX13 with the best fungal inhibitory effect was picked for the fungal confrontation experiment. When the fungus grows to about 1 / 3 of the size of the petri dish, inoculate bacteria at a distance of 1-2 cm from the fungus, and continue to culture in a greenhouse at 28 degrees Celsius for 2-3 days to observe the growth of the fungus.

[0062] Bacteriostatic rate=[(control fungal growth radius-treatment fungal growth radius) / control fungal growth radius]×100%

[0063] Experimental results: The confrontation test of LX13 against peanut crown rot was carried out for 3 times, as shown in Table 2, the inhibitory effect was obvious, the lowest inhibition rate was 75....

Embodiment 3

[0071] Example 3 Indoor Potted Plants of Bacterial Strains to Prevent and Control Peanut Diseases and Promoting Effect Test

[0072] After the strain was activated, it was inserted into LB liquid medium, placed in a shaker at 28 degrees at 180 r / min, and cultured for 3 days.

[0073] Propagation of preserved pathogenic bacteria of peanut crown rot, white silkworm and root rot: put oat grains into a triangular flask, soak in distilled water for 6 hours, pour off the water, and autoclave at 121°C for 20 minutes; Bacteria spp., S. spp., and root rot bacteria were inoculated into sterilized oat grains, cultured at 28°C for 7 days, and the inoculated bottle was shaken twice a day to make all oat grains have pathogenic bacteria, and the infected oat grains were obtained.

[0074] Pathogen inoculation: Peanuts (variety Luhua No. 8) were planted in pots in an artificial climate chamber, and the mass ratio of soil: vermiculite: nutrient soil was 2:1:1. The culture temperature is 28°C ...

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Abstract

The invention provides bacillus subtilis LX13 with the collection number of CGMCC No. 8805. The bacillus subtilis LX13 can be used for preventing and treating crown rot, southern blight and root rot of peanuts. Compared with the prior art, the bacillus subtilis LX13 has the advantages that the bacillus subtilis LX13 has obvious antagonism on pathogenic bacteria of the peanuts, and can serve as a potential biocontrol strain; the biocontrol effect is good, and the bacillus subtilis LX13 has obvious prevention and treatment effect on the crown rot, the southern blight and the root rot of the peanuts; growth and yield of the peanuts can be promoted remarkably; the bacillus subtilis LX13 is convenient to use, has quick action and long action time, and is easy to prepare and store and convenientto apply and popularize.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus subtilis and an application thereof. Background technique [0002] Peanut crown rot is a soil-borne fungal disease that can occur in the whole growth period of peanuts. The pathogen is Aspergillus nigerV.Tiegh, which belongs to the subphylum Deuteromycetes, Aspergillus genus Hyphospora, and Aspergillus niger fungus. The disease mainly occurs at the seedling stage or early growth stage. In the peanut seedling stage, the pathogen first infects the remaining cotyledon, and then infects the base of the peanut stem. The lesion is initially a yellowish-brown sunken lesion, and the edge of the lesion is brown. Later, the lesion expands rapidly, the cortex is longitudinally cracked, and the tissue is dry and rotted, and finally only broken fibrous tissue remains. In the case of humidity, the diseased part will soon grow a black mold layer, that is, the con...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C12R1/125
CPCA01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 迟玉成张霞许曼琳吴菊香董炜博王磊陈殿绪于建垒刘同金鄢洪海梁晨张茹琴
Owner SHANDONG PEANUT RES INST
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