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Rice sheath blight biocontrol strain RS-BC, microbial agent, preparation method and application

A technology of rice sheath blight and microbial inoculation, applied in the field of microbial inoculum, can solve the problems of no relevant literature, complicated prevention and control, and harm to non-target organisms

Inactive Publication Date: 2012-09-19
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rice sheath blight does not produce asexual spores, but exists in the form of hyphae or sclerotia and serves as the primary source of infection of rice. It survives for a long time in midwinter and still has a germination rate of 12% after being stored at room temperature for 11 years, and the sclerotia has no dormancy period and post-maturation period. It can germinate and infect pathogens under suitable conditions in the same year. The fungus at different development stages All sclerotia can germinate to cause disease and form new sclerotia. In addition, sclerotia has the ability to germinate and infect underground for many times (Tan Genjia et al., 2000). These characteristics of sclerotia have brought very complicated problems for control
[0004] At present, the application of chemical fungicides is still an effective means to control rice sheath blight, but it has irreparable defects: the use of broad-spectrum fungicides leads to soil environmental pollution, pathogenic bacteria develop resistance, harms non-target organisms, and leads to pesticide residues A series of problems such as these are not conducive to the promotion of green, environmentally friendly and pollution-free crop planting and the sustainable development of agriculture. Therefore, it is necessary to find a safe and effective alternative to prevent and control sheath blight
[0005] There is no relevant document related to the subject of the present invention so far

Method used

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  • Rice sheath blight biocontrol strain RS-BC, microbial agent, preparation method and application
  • Rice sheath blight biocontrol strain RS-BC, microbial agent, preparation method and application
  • Rice sheath blight biocontrol strain RS-BC, microbial agent, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Isolation and identification of strains

[0036] 1. The location and method of strain isolation

[0037] Rhizoctonia solani sclerotium was collected from the rice stalks in the rice test field of Huazhong Agricultural University in Shizishan District, Hongshan District, Wuhan City, Hubei Province. The sclerotia were soaked in sterile water for 1 min, and then the sclerotium was diluted with water and spread on the PDA plate. Incubate at 28°C. When a single colony grows, pick a single colony on the PDA plate for streaking culture, and observe the single colony morphology. A bacterial strain was obtained and designated as RS-BC. The single colony of this strain on the PDA medium was white and the surface was smooth. The RS-BC strain isolated as a result of Gram staining was a Gram-negative strain.

[0038] 2. 16S rDNA identification of the strain

[0039] Take the above-mentioned strain RS-BC and culture the colony in a PDB medium with shaking for 24 hours (rotating ...

Embodiment 2

[0042] Example 2: Analysis of the antagonism of the isolated strain RS-BC of the present invention

[0043] Using the confrontation culture method to determine the biocontrol bacteria RS-BC against Rhizoctonia solani strain WH-1 (Jiaotao Xie, Yanping Fu, Daohong Jiang, Guoqing Li, Junbin Huang, Bo Li, Tom Hsiang and Youliang Peng. Intergenic transfer of ribosomal genes between fungi. BMC Evolutionary Biology. 2008, 8: 87). Dip the RS-BC bacterial solution of the test strain with an inoculating loop, then lightly streak it in the center of the PDA plate, replace the RS-BC bacterial solution with sterile water as a control, set three replicates for each treatment, and culture at 37°C After incubating at a constant temperature for 12 hours, the edge of the colony of the rice sheath blight fungus strain WH-1 that has been activated on the PDA with a punch is used to take 5mm hyphae blocks and inoculate them on both sides of the strain RS-BC (such as figure 1 Shown), culture in a 37°C...

Embodiment 3

[0044] Example 3: Determination of the RS-BC antagonistic spectrum of the isolated strain of the present invention

[0045] The method of this embodiment is the same as that of embodiment 2, by figure 2 It can be concluded that the strain RS-BC of the present invention has a strong antagonistic effect on eight important plant pathogenic fungi including Magnaporthe grisea, Alternaria spp., Botrytis cinerea, among which the width of the inhibitory band against Fusarium oxysporum is the smallest, but still It can reach 2cm, and the width of the antibacterial band against rice blast fungus is the largest, 3.2cm, indicating that the RS-BC strain produces a broad spectrum of anti-biomass and has the potential to be widely used in the control of plant diseases.

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Abstract

The invention belongs to the technical field of biocontrol on plant diseases and particularly relates to separation and identification of a rice sheath blight biocontrol strain RS-BC, and a microbial agent containing the rice sheath blight biocontrol strain RS-BC, a preparation method of the microbial agent and application of the microbial agent to control of rice sheath blight. A separated strain is Burkholderia (Burkholderia vietnamiensis) RS-BC, which is collected in the China Center for Type Culture Collection with the collection number of CCTCC NO: M2011049. The invention further relates to the microbial agent prepared from the rice sheath blight biocontrol strain RS-BC, the preparation method of the microbial agent and the application of the microbial agent to the control of the rice sheath blight.

Description

Technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and specifically relates to the isolation and identification of a strain RS-BC for biological control of rice sheath blight, a microbial agent containing the biological control strain, a preparation method thereof, and the prevention and control of rice sheath blight Application in disease. Background technique [0002] Rice is the largest crop in the world, with an area of ​​154 million hectares, accounting for 11% of the world's arable land. Rice is also the basic food for at least half of the world's population. The sown area of ​​rice in my country accounts for 20% of the total sown area of ​​crops. It is the most important food crop and the largest crop in my country. The staple food of more than 40% of the population is rice, and rice consumption accounts for about 40% of the national food consumption. . However, pests and diseases severely reduce the yield and qual...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
Inventor 谢甲涛翟秋红姜道宏付艳苹程家森
Owner HUAZHONG AGRI UNIV
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