Citrus breed standard DNA fingerprint spectrum library and constructing method thereof

A technology of DNA fingerprinting and fingerprinting, which is applied in the field of fingerprinting database and its construction, can solve the problems of not establishing a DNA fingerprinting database of citrus varieties, etc., and achieve the effect of rapid detection and good repeatability

Inactive Publication Date: 2011-04-13
CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

There are also studies using molecular marker technology in citrus (Barkley et al. 2006; Pang et al. 2003; Liu Yong et al. 2005; Corzza-Nunes et al. 2002), but they all focus on the g

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  • Citrus breed standard DNA fingerprint spectrum library and constructing method thereof
  • Citrus breed standard DNA fingerprint spectrum library and constructing method thereof
  • Citrus breed standard DNA fingerprint spectrum library and constructing method thereof

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Embodiment Construction

[0041] The present invention is specifically described below through the examples, it is necessary to point out that the following examples are only used to further illustrate the present invention, and can not be interpreted as limiting the protection scope of the present invention, those skilled in the art can according to the above-mentioned present invention Contents Some non-essential improvements and adjustments are made to the present invention.

[0042] The construction of the standard DNA fingerprint library of citrus varieties is carried out as follows:

[0043] 1. Sequence acquisition of SSR characteristic primer pairs

[0044] Source of SSR primers: The primers were designed by Jiangdong et al. (2006) and previous reports (Kijas et al. 1995, 1997; Chen et al. 2006; Ahmad et al. 2003; Valdenice et al. 2006; Jiangdong et al. 2006) , which are distributed on different linkage groups, the primers are synthesized by Shanghai Sangon (Sangon), the purity of the pri...

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Abstract

The invention relates to a citrus breed standard DNA fingerprint spectrum library which is obtained according to the steps of extracting total DNA of various breeds of citrus, screening sequences of SSR (Simple Sequence Repeats) characteristic primer pairs, obtaining a citrus DNA characteristic fingerprint spectrum and constructing a citrus standard DNA fingerprint spectrum library. The citrus breed standard DNA fingerprint spectrum library has the advantages of good repeatability, stability and reliability and rapid detection when applied to distinguishing the citrus breeds, provides the basis to distinguish the citrus breeds by using an SSR molecule marker technology, can be directly applied to the true and false distinguishing of the citrus breeds, solves the difficulty of early distinguishing for the citrus nursery stock for a long term, and ensures that the citrus breeds are not limited by space-time conditions.

Description

technical field [0001] The invention relates to a fingerprint library and a construction method thereof, in particular to a citrus variety DNA fingerprint library and a construction method thereof. Background technique [0002] Citrus is the world's largest fruit, with a global planting area of ​​6.7 million hectares, an annual output of more than 100 million tons, and an annual trade volume of US$23.4 billion. It is the fourth largest agricultural product in international trade after wheat, corn and soybeans. my country is the country with the largest citrus cultivation area in the world, with a total output of 23 million tons of citrus, ranking first in the world. Over the past 30 years, hundreds of new citrus varieties have been bred abroad, while dozens of fine new citrus varieties have been bred in my country. However, at present, the management system of my country's citrus seedling industry is not perfect enough, and incidents of using inferior seedlings to fill up e...

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Application Information

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IPC IPC(8): C40B40/08C40B50/06C12N15/10C12Q1/68
Inventor 雷天刚陈善春何永睿姚利晓彭爱红许兰珍
Owner CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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