Method for preventing and treating candidatus liberibacter asiaticus by heat treatment

A citrus huanglongbing and citrus technology, which is applied in the field of preventing and treating citrus huanglongbing by heat treatment, can solve the problems such as the poor effect of citrus huanglongbing prevention and control technology, and achieve the effects of returning to normal fruit quality, increasing yield, and vigorous tree growth

Active Publication Date: 2013-11-27
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for preventing and treating citrus huanglongbing by heat treatment in order to solve the defect that the citrus huanglongbing disease prevention and treatment effect is not good in the prior art

Method used

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  • Method for preventing and treating candidatus liberibacter asiaticus by heat treatment
  • Method for preventing and treating candidatus liberibacter asiaticus by heat treatment
  • Method for preventing and treating candidatus liberibacter asiaticus by heat treatment

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Embodiment 1

[0035] There is a 200-square-meter net room in Changgang Mountain on the campus of South China Agricultural University.

[0036] Select citrus trees of various varieties, ages and disease degrees that have been infected with Huanglongbing as treatment objects. The processing object of this embodiment is to select 18 3-year-old infected tangerine potted seedlings in the autumn of 2011, and adopt the method of net room plastic film heat treatment to carry out heat treatment to the infected citrus potted seedlings. The specific steps are as follows:

[0037] S1. Build a frame: build a frame around the citrus potted seedlings infected with Huanglongbing;

[0038] S2. Covering with plastic film: Choose a sunny day with the highest daily temperature greater than 35°C, completely cover the periphery of the frame with plastic film, so that the internal temperature of the film reaches 45-52°C, and place the citrus tree in an environment of 45-52°C for continuous After 2 to 5 hours of ...

Embodiment 2

[0044] In the spring of 2012, 24 3-year-old potted seedlings of Shatangju were selected for heat treatment in a temperature control box. The specific heat treatment method was as follows: the 24 seedlings were randomly assigned to 4 treatments: 42°C, 46°C, 50°C And control, wherein each temperature is treated once a week, every time 2 hours, and is treated 4 times in a row, that is, 4 weeks of continuous treatment. After the high temperature treatment, the potted seedlings are taken out from the temperature control box and placed at ambient temperature for cultivation.

[0045] Samples were collected 30 days, 60 days and 150 days after the treatment, and Real time PCR was used to detect the pathogenic content of Huanglongbing in the samples. The results showed that there was no significant difference in the CT values ​​of the control group and the 42°C treatment group before and after several months of treatment (P>0.05); while the CT values ​​of the 46°C and 50°C treatment gr...

Embodiment 3

[0050] Yangcun, Boluo County, Huizhou City, Guangdong Province is the hardest-hit area for citrus Huanglongbing. The Yangcun Citrus Farm established in 1951 is one of the famous citrus fields in the country. By the early 1970s, its scale reached 20,000 mu (1 million plants) tree), in 1972 the overall profit reached 1.45 million yuan. However, since 1974, Huanglongbing became popular, and it occurred seriously from 1977 to 1982, which eventually led to the destruction of large areas of citrus orchards, and scattered citrus orchards began to disperse to individual businesses. In 2011, our laboratory cooperated with Guan Meiying, a fruit farmer in Yangcun, to carry out heat treatment experiments on the basis of ensuring the normal management of the orchard. Select 24 strains of 8-year-old sweet orange tree bodies that are severely morbid as experimental materials, and process 12 of them with the method of plastic film covering heat treatment; the method concrete steps of plastic ...

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Abstract

The invention specifically discloses a method for preventing and treating candidatus liberibacter asiaticus by heat treatment. The method comprises the specific steps as follows: a citrus tree body infected with candidatus liberibacter asiaticus is subjected to continuous heat treatment for 2-5 hours in the environment at the temperature of 45 DEG C-52 DEG C; then the tree body is cultivated at the environment temperature; and the operation is repeated in the next week, and continuous treatment is performed for 3-4 weeks. The method is economical and environment-friendly, the content of pathogenic bacteria in the infected tree can be greatly reduced or eliminated, the tree vigor of the experimental tree is recovered, the tree grows normally, and production and yield of citrus are obviously improved.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a method for preventing and treating citrus huanglongbing by heat treatment. Background technique [0002] Citrus Huanglongbing is the most serious and devastating disease in citrus production. The disease was first discovered in Chaoshan area of ​​Guangdong, my country in 1919, and has occurred and harmed more than 50 countries and regions so far. Among the 17 citrus producing areas in my country, 11 are under the threat of Huanglongbing, and Guangdong, Guangxi, Fujian, Yunnan, and Hainan, which are the main citrus producing areas, are particularly serious. Typical symptoms of affected citrus plants are "yellow shoots", mottled and yellowed leaves, smaller fruit, and the formation of "green fruit" or "red nose fruit" with no economic value. In the late stage of the disease, the root system is necrotic, and the tree vigor declines, resulting in leaf drop, f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00A01G1/00
Inventor 邓晓玲许美容关磊郑正梁美丹夏育陆
Owner SOUTH CHINA AGRI UNIV
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