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Method for transferring citrus cytoplasm male sterility character

A technology for cytoplasmic male sterility and male sterility, applied in horticultural methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve problems such as time-consuming, difficult artificial mutagenesis, and restrictions on wide application, and achieve The effect of improving efficiency

Inactive Publication Date: 2009-07-08
HUAZHONG AGRI UNIV
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Problems solved by technology

However, there are few natural autotetraploids in citrus, and artificial mutagenesis is also relatively difficult, and autotetraploids often have low fertility, which limits the wide application of this breeding approach, and this approach takes a long time. Soost et al. Variety took almost 20 years

Method used

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  • Method for transferring citrus cytoplasm male sterility character
  • Method for transferring citrus cytoplasm male sterility character
  • Method for transferring citrus cytoplasm male sterility character

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Experimental program
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Embodiment 1

[0037] 1. Preparation of parent materials:

[0038] Establishment of citrus embryogenic suspension lines: Satsuma citrus embryogenic callus preserved on MT solid medium (refer to "Content of the Invention" for medium composition) is selected for liquid suspension culture with vigorous growth, fine particles, light yellow color and loose tissue Liquid suspension culture was carried out in basal culture (MT minimal medium + 500mg / LME (Malt extract) + 1.5g glutamine + 40g / L sucrose, pH 5.8). The subculture period is 12-14 days, the culture condition is dark culture, the temperature is controlled at about 28°C, and the shaker speed is controlled at about 115rpm. After 3 subcultures, they were used for the isolation of parental protoplasts.

[0039] Preparation of mesophyll parents: take the mature seeds of the nucleated citrus varieties described in Table 1, soak them in 1% NaOH for 5-10 minutes, stir them several times with a glass rod in the middle to remove the pectin, wash th...

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Abstract

The invention belongs to the field of plant new species breeding technique, specifically to a method of transferring citrus cytoplasm male sterility trait, which is characterized in that based on the phenomenon that the citrus cell symmetrical amalgamation can regenerate diploid diachyma parent-type cybrid and its constituting features at molecule level, the cytoplasm male sterility trait controlled by the mitochondrial genome is transferred by amalgamation compounding matching and the cybrid obtained by culturing, thereby improving the diploid nuclear or multi-core citrus breeds. The invention discloses the symmetric amalgamation between cytoplasm male sterility Wenzhou Satsuma and Chinese characteristic nuclear or multi-core citrus, successfully invents a batch of diploid cybrids, realizes the effective transfer of Wenzhou Satsuma mitochondrial genome, and provides a novel method and new germplasm materials for diploid Satsuma breeding.

Description

technical field [0001] The invention belongs to the technical field of new plant variety breeding, and in particular relates to a method for transferring the cytoplasmic male sterility of citrus and creating a new diploid seedless citrus variety. Background technique [0002] Citrus is the most important fruit tree in the world and in southern my country. At present, my country's citrus cultivation area and output rank first in the world. The citrus industry covers the vast mountainous areas and reservoir areas in southern my country, and exerts huge economic, social and ecological benefits. While my country's citrus industry has made outstanding achievements, it is also facing some problems. In terms of varieties, citrus varieties in my country can be divided into two categories, one is imported varieties, which will face huge competitive pressure in export; There are many characteristic varieties, but the only shortcoming is that most varieties have seeds, which affects t...

Claims

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Application Information

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IPC IPC(8): C12N15/05A01H4/00G01N15/10C12Q1/68
Inventor 郭文武蔡小东付婧程运江邓秀新
Owner HUAZHONG AGRI UNIV
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