Lycium barbarum phylloxera attractant and application of lure containing attractant

Abstract

The invention discloses a Chinese wolfberry phylloxera attractant and application of a lure containing the attractant. Lycium barbarum phylloxera is one of main pests of lycium barbarum. According to the application disclosed by the invention, volatile matters of the overground part of Chinese wolfberry are taken as a research starting point, the (1S)-(-)-camphor is found to have an obvious trapping effect on the Chinese wolfberry phylloxera, and the trapping core containing the (1S)-(-)-camphor shows an obvious trapping effect on the Chinese wolfberry phylloxera in field trapping effect evaluation. Therefore, the (1S)-(-)-camphor can be used as the phylloxera lycephala attractant, and the lure containing the attractant can be used for trapping the phylloxera lycephala, so that the dependence on chemical pesticides is reduced, and the green prevention and control requirements are met.

Description

Technical Field

[0001] The invention belongs to the field of biological pesticides and relates to biological trapping of insects, in particular to a Lycium barbarum thread-horned psyllid attractant and use of an attractant core containing the attractant. Background Art

[0002] The goji berry threadworm, Bactericera gobica Loginova, is a major pest in goji berry production areas in Ningxia. It, along with the goji berry aphid, Aphissp., and the goji berry gall mite, Aceria pallida Keifer, are known as the "three major pests" of goji berries. They occur year after year, piercing and sucking damage to tender leaves, flower buds, and young fruits, causing leaf wilting, flower and fruit drop, and severely reducing goji berry yield and quality. During their infestation, the threadworm also secretes honeydew, which contaminates the leaves, causing mildew and impairing photosynthesis.

[0003] For a long time, the control of the wolfberry threadworm has relied primarily on chemical pesticides. This has led to the continuous increase in the threadworm's resistance to pesticides and the widespread killing of its natural enemies. This has disrupted the beneficial-harm balance, resulting in the frequent, multiple, and complex outbreaks of the threadworm, and the persistent nature of research on its control. With control effectiveness difficult to improve and control costs rising year by year, this has led to serious pollution from pesticide residues and a huge waste of pesticide investment. Control is becoming increasingly difficult and the damage is becoming increasingly serious. The harm caused by the threadworm and its chemical control have become a major bottleneck for the sustainable development of the wolfberry industry.

[0004] Attraction and control technology is a viable green approach for the control of the wolfberry threadworm. Herbivorous insects feed by identifying distinctive odors in their host plants, which act as signal molecules for the insects to locate their host plants. Leveraging these odors to attract the wolfberry threadworm, thereby achieving effective control, can reduce pesticide use and mitigate insecticide resistance. However, there is currently no effective attractant for the wolfberry threadworm.

[0005] In order to solve the above problems, the present invention is proposed. Summary of the Invention

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a Lycium barbarum Psyllid attractant and uses of an attractant core containing the attractant.

[0007] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0008] The invention relates to the use of (1S)-(-)-camphor in preparing a psyllid attractant for Chinese wolfberry bark.

[0009] A lure containing (1S)-(-)-camphor is used for trapping Lycium barbarum threadworm.

[0010] Preferably, the bait core uses (1S)-(-)-camphor as an active ingredient and is made into a bait core for trapping Lycium barbarum threadworm through a carrier acceptable in the field of insect trapping.

[0011] More preferably, the carrier is a natural desulfurized rubber stopper, and the method for preparing the lure comprises: first dissolving (1S)-(-)-camphor in an organic solvent to obtain a (1S)-(-)-camphor solution, then uniformly dropping the (1S)-(-)-camphor solution onto the natural desulfurized rubber stopper to ensure uniform absorption, sealing and packaging with aluminum film, and storing at low temperature.

[0012] More preferably, the organic solvent is n-hexane.

[0013] More preferably, the low temperature is 4°C.

[0014] Beneficial effects:

[0015] The wolfberry thread-eater psyllid is one of the main pests of wolfberry. The present invention uses the volatiles of the aboveground parts of wolfberry as the starting point for research and finds that (1S)-(-)-camphor has a significant luring effect on the wolfberry thread-eater psyllid. In the field trapping effect evaluation, the lure core containing (1S)-(-)-camphor showed a significant trapping effect on the wolfberry thread-eater psyllid. Therefore, (1S)-(-)-camphor can be used as a wolfberry thread-eater attractant, and the lure core containing this attractant can be used to trap the wolfberry thread-eater psyllid, reducing dependence on chemical pesticides and meeting the needs of green prevention and control. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is the GC-EAD reaction diagram of Lycium barbarum L. to host volatiles;

[0017] Figure 2 This is the GC-MS spectrum of wolfberry volatiles;

[0018] Figure 3 The GC-EAD reaction of Lycium barbarum Linehorn Psyllid with the standard substance 2-decalinone;

[0019] Figure 4 The GC-EAD reaction of Psyllidaceae with the standard substance (1S)-(-)-camphor;

[0020] Figure 5 Synthesize camphor GC-EAD reaction for the standard of Psyllidaceae;

[0021] Figure 6 This is the EAG response of Lycium barbarum to different concentrations of (1S)-(-)-camphor and 2-decalinone;

[0022] Figure 7 The results of the Y olfactometer behavioral test of compounds to which the adults of Lycium barbarum Linearis were sensitive;

[0023] Figure 8 This is a diagram evaluating the trapping effect of (1S)-(-)-camphor on the wolfberry thread psyllid. DETAILED DESCRIPTION

[0024] The essential contents of the present invention are described in detail below with reference to the embodiments, but the protection scope of the present invention is not limited thereto.

[0025] Example 1: Screening of attractants for the olfactory system of adults of the Lycium barbarum lineata

[0026] 1. GC-EAD analysis of volatiles from the host plant of Lycium barbarum

[0027] The volatiles from the aboveground part of the host plant of the wolfberry line psyllid were extracted by headspace adsorption method, and the antennae of the wolfberry line psyllid were tested by GC-EAD reaction. The results are as follows: Figure 1 As shown. Figure 1 It can be seen that the reactions of the antennae of the wolfberry thread-horned psyllid are all weak. In contrast, there is a stronger reaction at time 7.132 minutes, and it can be repeated on multiple antennae.

[0028] 2. GC-MS identification of wolfberry volatiles

[0029] The volatile compounds of Ningqi No. 7 were detected by GC-MS (see Figure 2 ), there are 221 compounds in total. Comparing the GC-EAD results, it is clear that there are three volatile compounds that respond to the wolfberry threadworm, namely (1S)-(-)-camphor, 2-decalinone and synthetic camphor.

[0030] 3. Determination of the active compounds of the plant phytotoxicity of Lycium barbarum

[0031] The standards of the three active substances were tested by GC-EAD reaction. Figure 3 This is the GC-EAD reaction of Lycium barbarum Linehorn Psyllid with the standard substance 2-naphthalene ketone. Figure 4 The GC-EAD reaction of the wolfberry line angle psyllid with the standard substance (1S)-(-)-camphor, Figure 5 A GC-EAD reaction was performed to synthesize camphor as a standard. Combined with GC-MS analysis, the compounds with behavioral activity in the worm were identified by comparison with the spectra of volatile substances from wolfberry plants. The results showed that the worm exhibited electrophysiological responses to small amounts of the standard substances 2-decalinone and (1S)-(-)-camphor. Comparison with the mass spectra of wolfberry plants revealed that the presence of 2-decalinone, (1S)-(-)-camphor, their mixtures, and small amounts of compounds in the wolfberry plant mixture overlapped with those in the wolfberry plant mixture.

[0032] 4. EAG verification

[0033] The above two compounds ((1S)-(-)-camphor and 2-decalinone) were diluted to 0.01μg / μL, 0.1μg / μL, 1μg / μL, and 10μg / μL concentration gradients (solvent was n-hexane). The antennal EAG response under different concentration gradients was measured, and n-hexane was used as a control. The results are shown in Figure 2. Figure 6 As shown in the results, the antennal potential (EAG) response of (1S)-(-)-camphor was the strongest at 10 μg / μL, which was significantly higher than that of 2-naphthalene (p<0.05), indicating that adults were more sensitive to (1S)-(-)-camphor.

[0034] 5. Behavioral measurement

[0035] The above two compounds ((1S)-(-)-camphor and 2-decalinone) were subjected to Y olfactometer behavioral assay. The results are as follows: Figure 7 As shown, (1S)-(-)-camphor and 2-decalinone have direct behavioral activity against the wolfberry threadworm, with adults showing significant selectivity for (1S)-(-)-camphor over the control (p<0.01). At a concentration of 10 μg / μL, the selectivity of wolfberry threadworm adults for (1S)-(-)-camphor was significantly higher than for 2-decalinone, reaching a high selectivity of 62.8%.

[0036] Example 2: Preparation of (1S)-(-)-camphor attractant core

[0037] The molecular formula of (1S)-(-)-camphor is C 10 H 16 O, CAS number 000464-48-2. Dissolve (1S)-(-)-camphor in n-hexane to obtain an attractant concentration of 10 μg / μL. Remove blank rubber stopper lures from their packaging and place them in groups of 10 into 100 mL conical flasks. Add 100 μL of the attractant dropwise to each conical flask, stirring with a glass rod to ensure uniform absorption. This results in a single lure loaded with 100 μg of the active ingredient. Seal the lures with 10 aluminum foil sheets per bag and store at 4°C.

[0038] Example 3: Field trapping effect evaluation

[0039] The blank rubber plug lure was soaked in 95% alcohol and water for cleaning, and then dried in the shade for later use. According to the method of Example 2, the (1S)-(-)-camphor attractant lure was prepared, and a negative control (equal volume of n-hexane was added, ck) was set up at the same time. The experiment used a white plastic sticky insect board (20cm×25cm) to eliminate the effect of color on the tropism of the wolfberry thread-cornered psyllid. The rubber plugs with different treatments were hung in the center of the sticky insect board with iron wire, and hung at a height of about 1m on the wolfberry plant, with the hanging direction including upwind and downwind. A row of wolfberry plants was used as a block, and only one set of repetitions was set in each block. The different treatments in each block were completely randomly set, and the intervals between different blocks were more than 15 meters.

[0040] Before the experiment, blank sticky traps without rubber stoppers were hung at the selected experimental site (the Bairuiyuan Wuzhong Hongsibao wolfberry cultivation base in Ningxia). One day later, the number of blank sticky traps in all experimental plots was counted and statistically analyzed. Only after confirming that there were no significant differences in initial insect populations between plots could the experiment be continued in that plot. Sticky traps with different rubber stoppers were then hung to investigate the effects of different lures on the attraction of the wolfberry thread-horned psyllid. Sticky traps and lures were replaced daily, with the positions of the different treatments altered daily. After one day of hanging, the number of female and male wolfberry thread-horned psyllids captured on both sides of the traps, as well as the total number of traps, was recorded and analyzed. Each treatment consisted of one sticky trap, representing a replicate, for a total of 14 traps, and the experiment was conducted continuously for 9 days.

[0041] The number of male, female and total insects of Lycium barbarum thread-necked psyllids captured on sticky insect boards was recorded daily and analyzed. The data on the total number of insects, female and male insects attracted were first tested to see if they conformed to the normal distribution. The data that conformed to the normal distribution were analyzed using one-way ANOVA. The data that did not conform to the normal distribution were tested using the non-parametric Kruskal-Wallis H test to see if there were significant differences among different treatments. The Mann-Whitney test was used to perform multiple comparisons among different treatments.

[0042] The experimental results are as follows Figure 8 As shown, the number of adults of Lycium barbarum lineata attracted by (1S)-(-)-camphor was significantly higher than that of the control group.

[0043] In summary:

[0044] The present invention uses volatile compounds from the aerial parts of wolfberries as a research starting point and finds that (1S)-(-)-camphor has a significant attractant effect on wolfberry threadfin mites. Lures containing (1S)-(-)-camphor showed a significant trapping effect on wolfberry threadfin mites in field trapping evaluations. Therefore, (1S)-(-)-camphor can be used as a wolfberry threadfin mites attractant, and lures containing this attractant can be used to trap wolfberry threadfin mites, reducing reliance on chemical pesticides and meeting the needs of environmentally friendly control.

[0045] The purpose of the above embodiments is to specifically introduce the essential content of the present invention, but those skilled in the art should know that the protection scope of the present invention should not be limited to this specific embodiment.

Claims

1. Use of (1S)-(-)-camphor in the preparation of a psyllid attractant.

2. A lure containing (1S)-(-)-camphor for catching Lycium barbarum threadworm.

3. The use according to claim 2, characterized in that: The lure core is made of (1S)-(-)-camphor as an active ingredient and a carrier acceptable in the field of insect trapping, so as to be used as a lure core for trapping wolfberry threadworms.

4. The use according to claim 3, characterized in that: The carrier is a natural desulfurized rubber stopper.

5. The use according to claim 4, characterized in that The preparation method of the lure comprises: firstly dissolving (1S)-(-)-camphor in an organic solvent to obtain a (1S)-(-)-camphor solution; then uniformly dropping the (1S)-(-)-camphor solution onto a natural desulfurized rubber stopper to ensure uniform absorption; sealing the lure with an aluminum film; and storing the lure at a low temperature.

6. The use according to claim 5, characterized in that: The organic solvent is n-hexane.

7. The use according to claim 5, characterized in that: The low temperature is 4°C.

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