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Complex insecticide of tung tree leaf extractive and Metarhizium anisopliae and preparation method thereof

A technology of Metarhizium anisopliae and its extract, applied in botany equipment and methods, insecticides, biocides, etc., can solve problems such as unused, discarded, and leaf shedding, and achieve poor quick-acting, waste reduction, and The effect of small environmental impact

Inactive Publication Date: 2014-06-18
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] On the other hand, the leaves of the deciduous tree tung tree fall off to the ground every autumn, and have been abandoned and unused for a long time.

Method used

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  • Complex insecticide of tung tree leaf extractive and Metarhizium anisopliae and preparation method thereof
  • Complex insecticide of tung tree leaf extractive and Metarhizium anisopliae and preparation method thereof
  • Complex insecticide of tung tree leaf extractive and Metarhizium anisopliae and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The compound insecticide in this example is prepared from the following raw materials in mass percentage: 60% of tung tree leaf extract; 10% of spore powder of Metarhizium anisopliae; 10% of diatomite; 15% of polyoxypropylene ether; -400 5%.

[0028] The tung leaf extract is prepared according to the following method:

[0029] (1) Dry the tung tree leaves, crush them with a pulverizer, pass through a 200-mesh sieve, add methanol and acetone mixed extract (the volume ratio of methanol and acetone is 2:1), heat to 70°C and reflux for three times, The volume (ml) of the mixed extract of methanol and acetone added each time: the mass of tung tree leaf powder (g)=6:1, the extraction time of each reflux is 3 hours, after combining the extracts each time, use a rotary evaporator Carry out vacuum distillation to remove methanol and acetone until methanol and acetone are all volatilized to obtain tung tree leaf methanol and acetone mixture extract extract; volatilized methanol ...

Embodiment 2

[0048] The difference between this embodiment and Example 1 is that the mass ratio of each raw material component is: 50% of the tung leaf extract, 50% of the tung chafer metarhizium anisopliae ( Metarhizium anisopliae ) spore powder (the concentration of live spores is 10 10 pcs / g) 20%, talc powder 10%, sodium lauryl sulfate 10%, dispersant Z 10%.

[0049] The efficacy of compounded insecticides in this example is shown in Tables 4-6.

[0050] (1) Toxic effect on diamondback moth

[0051] After diluting the compound insecticide of Example 2 with water in different times (1000 times, 1500 times, 2000 times), spray it onto the surface of cabbage leaves, and then raise live diamondback moths on the cabbage leaves to carry out the insecticidal efficacy test. Repeat three times, and set up 3 groups of control groups in addition: Control group 1: do not spray the composite insecticide of embodiment 2; Control group 2: spray the tung tree leaf extract insecticide diluted 1000 time...

Embodiment 3

[0063] The difference between the present embodiment and Example 1 is: the proportion of each raw material quality component is: 30% of the sieved material of the tung tree leaf extract passing through a 100 mesh sieve, Beauveria bassiana ( Beauveria bassiana ) spore powder (the concentration of live spores is 10 12 per g) 30%, kaolin 15%, sorbitol polyoxyethylene ether 10%, calcium lignosulfonate 15%.

[0064] The insecticidal effects of the compound insecticides in this example are shown in Tables 7-9.

[0065] (1) Toxic effect on diamondback moth

[0066]The compound insecticide of Example 3 was diluted with water in different times (1000 times, 1500 times, 2000 times), sprayed onto the surface of cabbage leaves, and then the live diamondback moth was raised on the cabbage leaves to carry out the insecticidal efficacy test. Repeat three times, set up 3 groups of control groups in addition: Control group 1: do not spray the composite insecticide of embodiment 3; Control g...

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Abstract

The invention relates to a complex insecticide of a tung tree leaf extractive and Metarhizium anisopliae and a preparation method thereof. The complex insecticide is prepared by blending the following raw materials by mass percent: 25%-65% of tung tree leaf extractive, 8%-32% of Metarhizium anisopliae conidial powder, 8%-20% of filling, 8%-20% of surfactant, and 4%-20% of insecticide adjuvant. The invention also comprises the preparation method of the complex insecticide. The complex insecticide provided by the invention has the advantages of high activity, stable effect, small influence on environment, rapidness of insecticidal efficacy and convenience in use, and can not only overcome the defect of poor fast-acting property of microbial pesticides, but also improve the insecticidal efficacy of a botanical insecticide. Experiments prove that after the complex insecticide diluted in 1000 times of water is sprayed, the average insecticidal rate can be over 84% after 72 hours.

Description

technical field [0001] The invention relates to a compound insecticide of botanical insecticide and microbial insecticide and a preparation method thereof, in particular to a compound insecticide of tung tree leaf extract and Metarhizium anisopliae and a preparation method thereof. Background technique [0002] Pesticides are widely used in various human production activities such as agriculture, forestry, and animal husbandry. With the development of modern science and technology, chemical pesticides are widely used because of their advantages such as wide adaptability, simple operation, low cost and remarkable effect. However, because of the extensive use of chemical pesticides, many new problems have been brought about, such as environmental pollution, pesticide residues, and resistance of pests and diseases. Due to the requirements of environmental protection and the proposal of green chemistry, modern pesticides should be non-polluting pesticides or pollution-free pest...

Claims

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Application Information

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IPC IPC(8): A01N65/18A01P7/04A01N63/04
CPCY02A50/30
Inventor 毛绍名章怀云孙汉洲刘高强
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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