Method for preparing biodiesel by grease biotransformation in solvent-free system

Abstract

The invention discloses a method for preparing biological diesel by converting biology to grease in a non-solvent system, belonging to the biological diesel composition technical field. In the non-solvent system, the invention converts all grease to produce the biological diesel by using Rhizopus chinensis CCTCC M201021 full cell lipase; all grease can be converted by batching fed-batch short chain alcohol, which prepares the biological diesel and the by product-glycerin, the yield of the biological diesel is 95 percent. The invention uses low cost grease which are mainly waste resources such as the unedible Jatropha curcas seed oil, the waster oil in the restaurant industry and the acid value oil, etc. as raw materials, thereby enlarging the material sources; because the Rhizopus chinensis CCTCC M201021 full cell lipase which is extremely low in cost is used as the catalyst, the problem of high price catalyst is effectively resolved; the toxic action of the alcohol on the biological catalyst is resolved by the batching fed-batch the short chain alcohol, thereby improving the conversion rate of the short chain alcohol. On the basis of the product quality reaching the international standard of the biological diesel, the cost of producing the biological diesel by the biological enzyme method is greatly lowered.

Description

technical field [0001] The invention discloses a method for preparing biodiesel from biotransformed oil in a solvent-free system, which belongs to the technical field of biodiesel synthesis. In particular, it relates to the new technology of Rhizopuschinensis CCTCC M201021 whole-cell lipase conversion of various oils to produce biodiesel with independent intellectual property rights in a solvent-free reaction system. Background technique [0002] Biodiesel refers to fatty acid monoesters prepared by transesterification of animal and vegetable oils and short-chain alcohols (methanol or ethanol). It is a renewable biofuel with properties very similar to diesel. It can not only be used as a substitute fuel for fossil diesel to alleviate the problem of increasing depletion of petroleum resources in recent years, but also has excellent characteristics such as incomparable environmental friendliness of fossil diesel. Currently, research on biodiesel has received extensive attentio...

AI Technical Summary

Problems solved by technology

[0004] 1. The process requires that the amount of methanol used greatly exceeds the reaction molar ratio, and the evaporation and reflux of excess methanol increases the energy consumption of the process;

[0005] 2. Free fatty acids and water are mixed in the reactants, which will hinder the transesterification reaction and seriously affect the progress of the reaction;

[0006] 3. The product must be washed with water. Alkali-containing waste liquid is produced during the washing process. If there is residual alkali, soap will be produced in the diesel oil, which is easy to block the pipeline

[0008] However, the biological method mainly has the following limitations: first, the price of the biocatalyst is expensive; second, methanol has a toxic effect on the biocatalyst; third, the conversion rate of methanol is not high, and the biggest obstacle to the commercialization of biodiesel produced by the enzymatic method is the preparation of the catalyst. The problem of high cost

However, commercial enzymes such as Novo 435 are expensive and not suitable for large-scale industrial production

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