Physic nut polyploid inducement method under the isolated culture condition

Abstract

The invention relates to a jatropha polyploidy induction method in the condition of isolated culture and is divided into such steps as jatropha aseptic seedling cultivation, polyploidy induction, polyploidy regenerated bud cultivation, selecting stable variants and rooting induction of variants in rooting cultivation medium containing active carbon, seedling adaptation in matrix and transport of seedling to land after survival. By adopting colchicines to induce the polyploidy of chromosome under isolated condition, the invention can easily control test conditions and repeat test result, thereby improving working efficiency, obtaining a plurality of plants with polyploidy chromosomes and reducing or avoiding heteroploidy cell chimerism. The invention also has actual significance in improving stress resistance of jatropha and can promote the development of renewable resources of jatropha.

Description

technical field

[0001] The invention relates to the technical field of tissue culture, in particular to a method for inducing polyploidy of Jatropha curcas under in vitro culture conditions. Background technique

[0002] Most plants in nature are diploid (2n=2x), that is, the nuclei of their somatic cells (2n) contain 2 identical chromosome sets (x). However, some species can form new species containing multiple chromosome sets through natural or artificial doubling of chromosomes, which we call polyploidy. Common polyploids are three, four, five, six and octaploid, etc., and 2n is equal to 3x, 4x, 5x, 6x and 8x respectively. Polyploidy is one of the most common characteristics of higher organisms, which is manifested as the number of chromosomes of closely related organisms in genetics. Polyploids are widely distributed in nature. According to statistics, about 1 / 2 of the species in the plant kingdom are polyploids, while polyploids in Poaceae account for almost 3 / 4, and ...