Lactobacillus and method for producing D-lactic acid by fermenting using lactobacillus

A kind of Lactobacillus and lactic acid technology, applied in the field of Lactobacillus and utilizing its fermentation to produce D-lactic acid, can solve the problems such as not involving the application of Lactobacillus pentosus, and achieve the effect of promoting nutritional needs

Active Publication Date: 2013-03-20
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But none of the above-mentioned patents involves the application of Lactobacillus pentosus in the production of D-lactic acid fermentation

Method used

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  • Lactobacillus and method for producing D-lactic acid by fermenting using lactobacillus
  • Lactobacillus and method for producing D-lactic acid by fermenting using lactobacillus
  • Lactobacillus and method for producing D-lactic acid by fermenting using lactobacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Put 0.1 g of lyophilized powder of Lactobacillus DMDL9010 into 5 mL of seed medium, and culture it statically at 30°C for 24 hours to make the first seed liquid;

[0043] (2) Put the first activation solution into the seed medium at a volume ratio of 10:100, and culture it statically at 30°C for 24 hours to make the second seed solution;

[0044] (3) Put the second activation solution into the seed medium at a volume ratio of 10:100, and culture it statically at 30°C for 24 hours to make the third seed solution;

[0045] (4) Put the third seed solution into the fermentation medium at a volume ratio of 5:100, and cultivate it at 30°C in a fermenter with a stirring speed of 100~150rpm. Add a total of 50g / L of sterilized CaCO in 5 times 3 , the fermentation time is 144h.

[0046] The formula of the seed medium is: glucose 20g / L, peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, sodium acetate 2g / L, triamine citrate 2g / L, Tween 80 1mL / L, MgSO 4 .7H 2 O 0.2 g / L,...

Embodiment 2

[0054] (1) Place 0.2 g of lyophilized powder of Lactobacillus DMDL9010 in 6 mL of seed medium, and culture it statically at 35°C for 20 hours to make the first seed liquid;

[0055] (2) Put the first activation solution into the seed medium at a ratio of 10:100 by volume, and culture it statically at 35°C for 24 hours to make the second seed solution;

[0056] (3) Put the second activation solution into the seed medium at a ratio of 10:100 by volume, and culture it statically at 35°C for 24 hours to make the third seed solution;

[0057] (4) Put the third seed liquid into the fermentation medium with a volume ratio of 8:100, and cultivate it at 35°C in a fermenter with a stirring speed of 100~150rpm. Add a total of 20g / L of sterilized CaCO2 in 2 times 3 After every 24 hours of fermentation, sterilized 40% (wt) ammonia water was added at a flow rate of 1.0ml / min, the pH was adjusted to 6.8, and the fermentation time was 120 hours.

[0058] The formula of the seed medium is: g...

Embodiment 3

[0064] (1) Put 0.3 g of lyophilized powder of Lactobacillus DMDL9010 into 7 mL of seed medium, and culture it statically at 30°C for 24 hours to make the first seed liquid;

[0065] (2) Put the first activation solution into the seed medium at a volume ratio of 10:100, and culture it statically at 30°C for 24 hours to make the second seed solution;

[0066] (3) Put the second activation solution into the seed medium at a volume ratio of 10:100, and culture it statically at 30°C for 24 hours to make the third seed solution;

[0067] (4) Put the third seed solution into the fermentation medium at a volume ratio of 5:100, and cultivate it at 30°C in a fermenter with a stirring speed of 100~150rpm. Add a total of 30g / L of sterilized Ca(OH) in 3 times 2 After every 24 hours of fermentation, sterilized 40% (wt) NaOH solution was added at a flow rate of 1.0 ml / min to adjust the pH to 7.0, and the fermentation time was 144 hours.

[0068] The formula of the seed medium is: glucose 1...

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Abstract

The invention discloses lactobacillus and a method for producing D-lactic acid by fermenting using the lactobacillus. A strain for the lactobacillus is lactobacillus (Lactobacillussp.) DMDL9010, and preservation number is CGMCC No.5172. The method comprises the following steps of: firstly, carrying out three-time activation culture on the strain; and then, fermenting for 108-144 hours at the temperature of 20 DEG C-35 DEG C to obtain D-lactic acid which is high in optical purity. According to the lactobacillus DMDL9010, D-lactic acid can be produced by fermenting pentose and hexose, and D-lactic acid is stable and high in yield, and the yield is 65-85g / L; produced D-lactic acid is high in optical purity which reaches 80-90%; the raw material adopted in the fermentation is wide in source and low in cost, and the fermenting cycle of the strain is just 108-144 hours; and D-lactic acid is produced by using the method, thus cost is saved, production efficiency is improved. The method disclosed by the invention has an important industrial application value.

Description

technical field [0001] The invention belongs to the technical field of organic acid fermentation, and relates to a production method of D-lactic acid, in particular to a lactobacillus and a method for producing D-lactic acid by fermentation thereof. Background technique [0002] Biomass refers to terrestrial and aquatic plants mainly composed of lignin, cellulose, hemicellulose and other organic matter. It is a stable renewable energy resource with rich sources. The annual output of crop straw in my country alone is about 700 million Ton. After the oil crisis in the last century, people began to pay attention to the technology of preparing lactic acid and fuel alcohol from biomass. [0003] Lactic acid (molecular formula: C 2 h 5 OCOOH), that is, α-hydroxypropionic acid, because the carbon atom at the α position of the carboxyl group in its molecule is an asymmetric atom, it has optical activity, and can be divided into two configurations, L (+) and D (-). L-lactic acid is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/225
Inventor 刘冬梅周劲松尚雍贺
Owner SOUTH CHINA UNIV OF TECH
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