The invention discloses a method for extracting fructus
cannabis oligopeptide from fructus
cannabis cake
meal. The method particularly comprises the following steps: collecting fructus
cannabis cake
meal with
grease content being less than 1%, adding water, adjusting the pH to 9.0, adding
sodium metabisulfite, performing
solid-liquid separation and collecting
liquid phase; adding a HCl solution into the
liquid phase, adjusting the pH to 3.8 to 4.3, standing, performing
solid-liquid separation for the second time and collecting precipitate; adding water, heating to 40 to 55 DEG C, adjusting thepH to 7.0 to 9.0, adding composite
protease, performing enzymolysis for 3 to 6 hours, performing
centrifugal filtration and collecting
clear liquid, namely a fructus cannabis
oligopeptide solution; performing electroosmosis, concentrating and freeze-
drying to obtain the fructus cannabis
oligopeptide powder. The fructus cannabis oligopeptide is obtained by taking the fructus cannabis cake
meal, which is squeezed to remove oil, as a
raw material with reference to an
isoelectric point principle and
protein precipitation, and according to the methods for performing enzymolysis by the composite
protease and freeze-
drying. In the whole process, a pure physical process is utilized, chemical components are not added basically, the cost is low, and environmental
pollution is avoided.