A method for preparing sialic acid and oligopeptide mixture using bird's nest as raw material

A technology of sialic acid and oligopeptide, which is applied in the field of food processing, can solve the problem that bird's nest degradation products are not easy to be degraded and absorbed, and achieve the effects of avoiding Maillard reaction, easy operation and simple process

Active Publication Date: 2021-10-08
姚之龙 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There is a certain amount of reducing sugar in bird's nest, which is released after traditional protease degradation, and further undergoes Maillard reaction with amino groups of polypeptides after protein degradation. Protease has a strong resistance to digestion and leads to the formation of colored substances, making bird's nest degradation products difficult to be degraded and absorbed

Method used

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  • A method for preparing sialic acid and oligopeptide mixture using bird's nest as raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Raw materials for a certain amount of feathers, hairs and other debris sawdust cup nests, wherein the total protein content of 49% of the sialic acid content of 10%, 9% moisture content, the total polysaccharide content of 28%, 4% ash, sawdust and feather and other debris content of about 9%.

[0025] (1) hair extract sialic acid nest 1: Weigh 1000g nest light hair, using 10kg, pH 8.0 aqueous NaOH solution was washed three times, then washed with deionized water to 2kg neutral, then it is added to 10kg, pH is 3.0 aqueous hydrochloric acid, soaking at 50 ℃ 6h, filtered with a 200 mesh screen were collected and the serum a of 7.5kg solids a is 5.4kg.

[0026] (2) Extraction gross nest sialic 2: Step (1) A solid obtained 5.4kg to 10kg resuspended in deionized water and beaten using a colloid mill, was added 10g of galactosidase added to the suspension, adjusting solution to pH 6.0, at normal pressure and enzymatic 6h stirring, centrifugation, the supernatant was collected and ...

Embodiment 2

[0031] Feed containing an amount of feathers and other debris broken nest of hair, wherein the total protein content of 47%, the sialic acid content of 9.8%, moisture content 10.1%, total polysaccharide content of 27%, 4% ash, sawdust and other debris feather content of about 11.7%.

[0032] (1) hair extract sialic acid nest 1: Weigh 1kg bird's nest broken hair, using 10kg, pH 7.5 aqueous solution of KOH washed four times and then washed with deionized water using 3kg to neutral, then it is added to 10kg, pH is 3.5 in aqueous acetic acid, soaked at 40 ℃ 8h, filtered with a 200 mesh screen were collected and the serum a of 7.7kg solids a is 5.1kg.

[0033] (2) Extraction gross nest sialic 2: Step (1) A solid obtained 5.1kg to 10kg resuspended in deionized water and beaten using a colloid mill, was added 10g mannosidase to the suspension, the solution was adjusted to pH 5.8, and digested under atmospheric pressure with stirring 5h, centrifugation, the supernatant was collected and B...

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Abstract

The invention discloses a method for preparing a mixture of sialic acid and oligopeptide by using bird's nest as a raw material, which is characterized in that it comprises the following steps: (1) extraction of sialic acid from bird's nest 1; (2) extraction of sialic acid from bird's nest 2; 3) extracting the oligopeptide from the bird's nest; (4) concentrating the extract and (5) drying. The method of the present invention has the advantages of simple process, easy operation and no miscellaneous smell in the product, and the prepared mixture is rich in bird's nest sialic acid and oligopeptide; the present invention can avoid the Maillard reaction and suppress the generation of colored substances, so that the prepared The mixture of sialic acid and oligopeptide is white or milky white.

Description

Technical field [0001] The present invention belongs to the field of food processing, in particular, the present invention relates to a process for extracting a raw material and the hair of the bird's nest by fractional enzymatic sialic acid and the like during the preparation of the mixture of oligopeptides. [0002] technical background [0003] Swiftlet bird's nest is Apodidae and a variety of animals belong to the same class swallow saliva and other condensation had built her nest, bird's nest after treatment after washing, to hair, molding is a valuable tonic boutique, with Yin dryness, fill in Qi, spleen and stomach, Huatanzhike and other functions, is regarded as valuable supplements and advanced feast on traditional dishes. Bird's nest is rich in protein, carbohydrates and minerals, wherein the protein about 50%, about 30% polysaccharide content, about 10% water, about 3% ash, and other trace elements. [0004] Nest protein is present in the form of glycoprotein, called as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/26C12P21/06
CPCC12P19/26C12P21/06
Inventor 姚之龙高建萍
Owner 姚之龙
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