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Method for preparing sialic acid and oligo peptide mixture with malaysia's bird nets as raw materials

A technology of sialic acid and oligopeptide, applied in the field of food processing, can solve the problem that bird's nest degradants are not easily degraded and absorbed, and achieve the effects of avoiding Maillard reaction, easy operation and simple process.

Active Publication Date: 2018-06-12
姚之龙 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There is a certain amount of reducing sugar in bird's nest, which is released after traditional protease degradation, and further undergoes Maillard reaction with amino groups of polypeptides after protein degradation. Protease has a strong resistance to digestion and leads to the formation of colored substances, making bird's nest degradation products difficult to be degraded and absorbed

Method used

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  • Method for preparing sialic acid and oligo peptide mixture with malaysia's bird nets as raw materials

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The raw material is bird's nest cup containing a certain amount of feathers, sawdust and other sundries, in which the total protein content is 49%, the sialic acid content is 10%, the water content is 9%, the total polysaccharide content is 28%, the ash content is 4%, feathers and sawdust The content of other sundries is about 9%.

[0023] (1) Extraction of sialic acid from bird's nest 1: Weigh 1000g of bird's nest cup, wash 3 times with 10kg of NaOH aqueous solution with a pH of 8.0, then wash with 2kg of deionized water until neutral, then add it to 10kg with a pH of 8.0 Soak in 3.0 hydrochloric acid aqueous solution at 50°C for 6 hours, filter with a 200-mesh sieve, and collect 7.5kg of clear liquid A and 5.4kg of solid A respectively.

[0024] (2) Bird's nest sialic acid extraction 2: the 5.4kg solid matter A that step (1) obtains is resuspended in 10kg deionized water, adopts colloid mill to carry out beating treatment, adds the galactosidase of 10g in the suspensi...

Embodiment 2

[0029] The raw material is broken bird's nest containing a certain amount of feathers and other sundries, of which the total protein content is 47%, the sialic acid content is 9.8%, the water content is 10.1%, the total polysaccharide content is 27%, the ash content is 4%, and feather sawdust and other sundries The content is about 11.7%.

[0030] (1) Extraction of sialic acid from bird’s nest 1: Weigh 1kg of bird’s nest, wash it 4 times with 10kg of KOH aqueous solution with a pH of 7.5, then wash it with 3kg of deionized water until neutral, then add it to 10kg with a pH of 7.5 Soak in 3.5 acetic acid aqueous solution at 40°C for 8 hours, filter through a 200-mesh sieve, and collect 7.7kg of clear liquid A and 5.1kg of solid A respectively.

[0031] (2) Extraction of sialic acid from bird's nest 2: resuspend 5.1kg of solid matter A obtained in step (1) into 10kg of deionized water, use a colloid mill for beating, add 10g of mannosidase to the suspension, and adjust the solut...

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Abstract

The invention discloses a method for preparing a sialic acid and oligo peptide mixture with malaysia's bird nets as raw materials. The method is characterized by including the steps that (1), malaysia's bird net sialic acid is extracted for the first time; (2), malaysia's bird net sialic acid is extracted for the second time; (3), malaysia's bird net oligo peptide is extracted; (4), an extractingsolution is concentrated; (5), drying is performed. The method has the advantages that the process is simple, the operation is easy, and the product is free of a foreign flavor; the prepared mixture has rich bird net sialic acid and oligo peptide. The method can avoid a maillard reaction and restrain generation of colored matter, and the prepared sialic acid and oligo peptide mixture has a white or milk white color.

Description

technical field [0001] The invention belongs to the field of food processing. Specifically, the invention relates to a method for preparing a mixture of sialic acid and oligopeptides by using bird's nest as a raw material through steps of extraction and enzymatic hydrolysis. technical background [0002] The bird's nest is a nest built by swiftlets of the Swift family Swiftlet family and many kinds of swallows of the same species with the condensation of saliva. It has the functions of nourishing Qi, invigorating the stomach and nourishing the spleen, resolving phlegm and relieving cough, etc. It is traditionally regarded as a precious tonic and a high-end banquet delicacy. Bird's nest is rich in protein, sugar and minerals, including about 50% protein, about 30% polysaccharides, about 10% water, about 3% ash and some trace elements. [0003] The protein in bird's nest exists in the form of glycoprotein, called sialoglycoprotein, which is a characteristic component of bird'...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26C12P21/06
CPCC12P19/26C12P21/06
Inventor 姚之龙高建萍
Owner 姚之龙
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