Method for extracting high-purity rice glutelin

An extraction method and glutenin technology, which is applied in the field of rice glutenin extraction, can solve the problems of dark brown substances and intensified Maillard reaction, and achieve the effect of avoiding Maillard reaction and reducing losses

Active Publication Date: 2015-11-25
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention aims to solve the problem of protein denaturation and hydrolysis caused by the existing method of extracting gluten with dilute alkali, intensified Maillard reaction, and the production of dark brown substances, and provides a method for extracting high-purity rice gluten

Method used

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  • Method for extracting high-purity rice glutelin
  • Method for extracting high-purity rice glutelin
  • Method for extracting high-purity rice glutelin

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specific Embodiment approach 1

[0027] Specific embodiment one: the extraction method of the high-purity rice glutenin of the present embodiment is carried out according to the following steps:

[0028] 1. Grinding rice into rice flour;

[0029] 2. Degreasing the rice flour with n-hexane for 3 to 5 times to obtain defatted rice flour;

[0030] 3. Remove albumin, globulin and prolamin from defatted rice flour to obtain precipitation;

[0031] Four, the precipitation that step 3 obtains is that the concentration of 0.8%~1.2% (w / v) extracts gluten 3~5 times with lactic acid;

[0032] 5. Combine the supernatant collected by each extraction, and use the TCA solution with a concentration of 8% to 12% (w / v) to carry out protein precipitation on the supernatant;

[0033] 6. Washing the precipitate with PBS buffer solution for 3 to 5 times, vacuum freeze-drying the washed precipitate to obtain rice gluten.

specific Embodiment approach 2

[0034] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the specific method of grinding rice into rice flour in step 1 is: grind fresh rice with a pulverizer for 15-20 seconds each time, 30-40 times in total. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0035] Specific embodiment three: what this embodiment is different from specific embodiment one is: in step 2, the concrete method that rice flour is degreased with n-hexane is:

[0036] A. Mix rice flour and n-hexane according to the ratio of 1g:4~6mL, stir at room temperature for 1h, let stand for 1h, and then centrifuge at 4000rpm for 20min to collect the precipitate;

[0037] B. Repeat step A for 2 to 4 times, and dry the collected precipitate in a water bath at 30° C. to obtain defatted rice flour. Others are the same as in the first embodiment.

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Abstract

The invention discloses a method for extracting high-purity rice glutelin, relates to a method for extracting rice glutelin, and aims at solving the problems of denaturation and hydrolysis of protein, intensified Mailard reaction and generated black brown substances in the existing method for extracting glutelin by virtue of diluted alkali. The method comprises the following steps: 1, grinding rice into rice flour; 2, degreasing the rice flour by normal hexane to obtain degreased rice flour; 3, removing albumin, globulin and prolamin from the degreased rice flour to obtain a sediment; 4, extracting glutelin from the sediment obtained in the step 3 by virtue of lactic acid; 5, mixing supernatants collected after digestion of each time, and carrying out protein precipitation on the mixed supernatant by a TCA solution; and 6, cleaning a sediment by a PBS buffer, and carrying out vacuum freeze drying on the cleaned sediment to obtain the rice glutelin. The rice glutelin extracted by the method disclosed by the invention has the purity as high as 93.67%-96.71%, and the method disclosed by the invention can be widely applied to large-scale industrialized extraction of rice glutelin.

Description

technical field [0001] The invention relates to a method for extracting rice gluten. Background technique [0002] Rice protein is mainly composed of albumin, globulin, prolamin and glutenin. Among them, glutenin mainly exists in the type II protein body (PB-Ⅱ), and the glutenin molecule basically consists of three subunits of 22KDa, 37~39KDa, and 57KDa. [0003] Studies have shown that glutenin-rich rice protein has a good effect on lowering cholesterol. Therefore, the effective extraction of rice glutenin will provide a theoretical basis for the development of functional rice protein in my country, and has extensive and far-reaching application significance. [0004] Most of the traditional methods use dilute alkali to extract glutenin, but the alkaline extraction has a destructive effect on amino acids. At the same time, the starch content in the extract is high, the extraction liquid-solid ratio is large, the isoelectric point precipitation consumes a lot of acid, and d...

Claims

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Application Information

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IPC IPC(8): A23J1/12
Inventor 杨林李慧王正旋刘烨梁明才
Owner HARBIN INST OF TECH
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