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Method for extracting fructus cannabis oligopeptide from fructus cannabis cake

A technology of hemp seed and oligopeptide, which is applied in the field of recovery and reuse of hemp seed cake, can solve the problems of complex separation process of hemp seed protein, affecting the color of hemp seed cake, complex preparation process, etc., and achieves Effects of preventing oxidation, increasing added value, and high yield

Inactive Publication Date: 2016-10-12
山西汉麻生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Hemp seeds contain phenolic substances that are easily oxidized, which will remain in the processed hemp seed cakes. After oxidation in the air, pink oxidation products will be produced, which affects the processing of hemp seed cakes. product color
In addition, besides protein, hemp seed cake also contains some monosaccharides, which makes the separation process of hemp seed protein more complicated.
[0004] The prior art only uses the acid-base extraction method to extract protein powder from the hemp seed, and the preparation process is complicated, and the product purity is low
And take the hemp seed cake as raw material to prepare the method for hemp seed oligopeptide and also have no report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Put 200kg of hemp seed cake (with oil content less than 1%) after crushing into 5m 3 In the extraction tank, the extraction tank is characterized by a sandwich type heating reaction kettle for dissolving the hemp seed cake powder. Add 4 tons of pure water to the extraction tank, adjust the pH value to 9.0 with 2.5mol / L NaOH solution, add 2kg of sodium bisulfite, heat and stir at 70°C for 30min, and centrifuge at 4000rpm for 20min in a decanter centrifuge with automatic deslagging to carry out solid-liquid Separation, clear liquid into 5m 3 Settling tank, the settling tank is a non-heating reaction kettle; use 2.5mol / L HCl solution to adjust the pH value to 4 to precipitate protein, stir evenly, let stand for 2h, and use an automatic slag unloading decanter centrifuge for the second solid-liquid Separate, discard the supernatant and leave the slag material, which is the hemp seed protein isolate.

[0026] (2) Crush the protein isolate and add 5m 3 In the extractio...

Embodiment 2

[0032] (1) Put 200kg of hemp seed cake (with oil content less than 1%) after crushing into 5m 3 In the extraction tank, the extraction tank is characterized by a sandwich type heating reaction kettle for dissolving the hemp seed cake powder. Add 4 tons of pure water to the extraction tank, adjust the pH value to 9.0 with 2.5mol / L NaOH solution, add 2kg of sodium bisulfite, heat and stir at 70°C for 30min, and use a plate and frame filter press for solid-liquid separation; the clear liquid is introduced into 5m 3 Settling tank, the settling tank is a non-heating reaction kettle, use 2.5mol / L solution to adjust the pH value to 4 to precipitate protein, stir evenly and let it stand for 2h, use plate and frame filter press to carry out solid-liquid separation for the second time, discard The supernatant remains slag material, and the slag material is hemp seed protein isolate.

[0033] (2) Crush the protein isolate and add 5m 3 In the extraction tank, add pure water according to...

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Abstract

The invention provides a method for extracting fructus cannabis oligopeptide from a fructus cannabis cake. The method comprises the following steps: collecting the fructus cannabis cake with the oil content of lower than 1%, adding water, regulating the pH value to 9.0, adding sodium metabisulfite, carrying out solid-liquid separation, and collecting the liquid phase; adding a HCl solution into the liquid phase, regulating the pH value to 3.8-4.3, standing, carrying out secondary solid-liquid separation, and collecting the precipitate; adding water, heating to 40-55 DEG C, regulating the pH value to 7.0-9.0, adding a compound protease, carrying out enzymolysis for 3-6 hours, carrying out centrifugal filtration, and collecting the clear solution which is the fructus cannabis oligopeptide solution; and carrying out electrodialysis, concentration and freeze-drying to obtain the fructus cannabis oligopeptide powder. The fructus cannabis cake subjected to press deoiling is used as the raw material, the isoelectric point principle is combined to precipitate the protein, and the compound protease enzymolysis and freeze-drying are performed to obtain the fructus cannabis oligopeptide. The whole process utilizes the pure physical technique, and no chemical component is added; and thus, the method has the advantages of low cost and no environment pollution.

Description

technical field [0001] The invention relates to the technical field of recovery and reuse of hemp seed cakes, in particular to a method for extracting hemp seed oligopeptides from the hemp seed cakes. Background technique [0002] Hemp seed is the seed kernel of Cannabis sativa L., a plant in the family Moraceae. It has the effects of moistening dryness and smoothing intestines, diuresis, dredging stranguria, and promoting blood circulation. It is a moisturizing medicine that is both medicine and food. Modern research shows that hemp seed has good effects of lowering blood pressure, labor pain, anti-inflammation, and anti-thrombosis. Long-term consumption has a good auxiliary effect on chronic neuritis, paralysis, ulcers, and gynecological diseases. Hemp seed cake is the residue after extracting hemp seed oil from hemp seeds, the protein content of which is as high as 56%, and contains various essential amino acids needed by the human body, especially rich in arginine, lysin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 包卫洋马普黄祥贤王祖哲张莹
Owner 山西汉麻生物科技有限公司
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