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Method for simultaneously separating chlorogenic acid and rutin from tobacco leaves

A technology of chlorogenic acid and rutin, applied in chemical instruments and methods, separation/purification of carboxylate, preparation of carboxylate, etc., to achieve the effect of simple operation, lower extraction cost and simple separation

Active Publication Date: 2017-03-15
CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, we have not seen the process report on the simultaneous separation of chlorogenic acid and rutin on the basis of sugar and pigment removal

Method used

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  • Method for simultaneously separating chlorogenic acid and rutin from tobacco leaves
  • Method for simultaneously separating chlorogenic acid and rutin from tobacco leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0030] 1. Simultaneous extraction of tobacco chlorogenic acid and rutin

[0031] Weigh 200g of tobacco leaf powder sample passed through a 60-mesh sieve, dissolve it in 4000ml of water at a material-to-liquid ratio of 1:20, pour it into a stirring extractor and set the extraction temperature at 50°C, and extract with stirring and extraction for 120 minutes. The obtained extract was subjected to suction filtration, the residue was filtered off, and the filtrate was collected. The extraction rates of tobacco chlorogenic acid and rutin in the filtrate reach 84% and 99% respectively (according to the experimental data of recovery rate). The contents of chlorogenic acid and rutin in the filtrate reached 21.7 mg / g and 16.5 mg / g, respectively. The filtrate is extracted with petroleum ether to remove impurities according to the volume ratio of 1:1, the upper organic phase is recovered, and the lower aqueous phase is combined to obtain the chlorogenic acid and rutin extract. At this ...

Embodiment 2

[0039] 1. Simultaneous extraction of tobacco chlorogenic acid and rutin

[0040] Weigh 200g of tobacco leaf powder sample passed through a 60-mesh sieve, dissolve it in 5000ml of water at a material-to-liquid ratio of 1:25, pour it into a stirring extractor and set the extraction temperature at 50°C, and extract with stirring and extraction for 120 minutes. The obtained extract was subjected to suction filtration, the residue was filtered off, and the filtrate was collected. The filtrate is extracted with petroleum ether to remove impurities according to the volume ratio of 1:1, the upper organic phase is recovered, and the lower aqueous phase is combined to obtain the chlorogenic acid and rutin extract.

[0041] 2. Separation of tobacco chlorogenic acid and rutin

[0042] 1. Isolation of chlorogenic acid

[0043] Put the pretreated HPD‐100 type macroporous resin into the chromatography column, so that the column surface is flat, without inclination, without air bubbles in t...

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Abstract

The invention discloses a method for simultaneously separating chlorogenic acid and rutin from tobacco leaves. The method includes: weighting 200g of tobacco leave powder sieved through a 60 mesh sieve, dissolving the tobacco leave powder in water according to a material-liquid ratio of 1:20, setting temperature as 50 DEG C, and stirring and extracting the chlorogenic acid and the rutin for 120min. Petroleum ether is used for extraction and removal of oil-soluble impurities for collected filtrate, and then a chlorogenic acid and rutin extracting solution is obtained. Column chromatography isolation of the chlorogenic acid and the rutin is performed on the upper column of the extracting solution. Water of 5 times of the extracting solution in volume is first used for elution until no sugar can be detected in eluate. After the water is used for the elution, different concentration of ethanol replaces the used eluate for gradient elution. Two types of ethanol eluate 10-15% and 30-40% in concentration are respectively collected so as to obtain a chlorogenic acid solution and a rutin solution. The method for simultaneously separating the chlorogenic acid and the rutin from the tobacco leaves saves raw material and reagents, reduces development cost, and achieves good separating effects.

Description

technical field [0001] The invention belongs to the technical field of extraction of effective plant components, and relates to a method for simultaneously extracting and separating tobacco chlorogenic acid and rutin from tobacco leaves. Background technique [0002] my country's tobacco planting area and output both rank first in the world, and the annual production of tobacco leaves is more than 2 million tons. According to statistics, more than 700 billion yuan of profits and taxes were paid to the state in 2013. Therefore, tobacco production and cigarette processing occupy a very important position in my country's national economy. However, affected by global climate change, frequent natural disasters and the "Framework Convention on Tobacco Control" advocated by the World Health Organization in recent years, tobacco companies have overstocked tobacco leaves, and about 30% of low-grade tobacco leaves cannot be used for cigarette production. According to statistics, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C67/48C07C67/56C07C67/58C07C69/732C07H1/08C07H17/07
CPCC07C67/48C07C67/56C07C67/58C07H1/08C07H17/07C07C69/732
Inventor 彭曙光王征许诗豪杨红旗杨红武
Owner CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION
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