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A kind of production technology of cowhide collagen peptide

A production process and original protein technology, which is applied in the production process of cowhide collagen peptides, can solve the problems of irreversible loss of products, decreased activity of active peptides, and unpleasant sensory sensations, and achieve high biological antioxidant activity and reduce the risk of decolorization Requirements, Effects of Avoiding Protein Peptide Loss

Active Publication Date: 2022-06-28
湖北瑞邦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) Domestic cowhide raw materials generally have many kinds and miscellaneous situations. There are many origins of cowhide, resulting in uneven quality of cowhide, which directly affects the quality of subsequent production products. A lot of raw materials are wasted, and the quality of the product cannot be effectively guaranteed;
[0005] (2) Sterilization generally adopts ultra-high temperature method, which will deepen the color of the product, and will lead to the decline or even loss of the activity of the active polypeptide in the product, affecting its functionality;
[0006] (3) Activated carbon powder is generally used in the decolorization process, which will cause irreversible loss of the product (polypeptide) while removing pigment substances;
[0007] (4) The produced collagen peptide has an unpleasant smell and poor flavor

Method used

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  • A kind of production technology of cowhide collagen peptide
  • A kind of production technology of cowhide collagen peptide
  • A kind of production technology of cowhide collagen peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A production process of bovine skin collagen peptide, comprising the following steps:

[0040] S1 cleaning: wash 100 parts of cowhide with clean water, immerse the washed cowhide in 1400 parts of water, add NaOH to adjust the pH value to 13, add 1.35 parts of H 2 O 2 , soaked for 78h;

[0041] S2 denaturation: clean the clean cowhide obtained in step S1 until the pH is neutral, then cut and grind it to make cowhide slurry, add the cowhide slurry into water with 5 times the mass fraction of the cowhide slurry, and set the holding temperature during the denaturation to 72°C, the holding time is set to 5.5h, and the denatured cowhide protein slurry is obtained;

[0042] S3 enzymatic hydrolysis: add 3.75 parts of alkaline protease to the denatured cowhide protein slurry obtained in step S2 denaturation, keep the temperature at 57 °C, keep the pH at 8.2, and react for 5.5 hours. During the enzymatic hydrolysis, use ultrasonic assisted enzymatic hydrolysis, ultrasonic wave ...

Embodiment 2

[0048] A production process of bovine skin collagen peptide, comprising the following steps:

[0049] S1 cleaning: wash 100 parts of cowhide with clean water, immerse the washed cowhide in 1000 parts of water, add NaOH and Na 2 CO 3 Adjust the pH value to 10, add 2 parts of stable chlorine dioxide, soak for 50h;

[0050] S2 denaturation: clean the clean cowhide obtained in step S1 cleaning until the pH is neutral, then cut and grind to make cowhide slurry, add the cowhide slurry into water with 4 times the mass fraction of the cowhide slurry, and set the holding temperature during denaturation to 60-80 ℃, the holding time is set to 6h, to obtain the denatured cowhide protein slurry;

[0051] S3 Enzymatic hydrolysis: Add 2 parts of alkaline protease to the denatured cowhide protein slurry obtained in the denaturation of step S2, keep the temperature at 65 °C, keep the pH at 7.5, and react for 6 h. During the enzymatic hydrolysis, ultrasonic-assisted enzymatic hydrolysis is us...

Embodiment 3

[0057] A production process of bovine skin collagen peptide, comprising the following steps:

[0058] S1 cleaning: Wash 100 parts of cowhide with clean water, immerse the washed cowhide in 2,000 parts of water, add NaOH and CaO to adjust the pH to 14, add 0.5 part of sodium hypochlorite, and soak for 200h;

[0059] S2 denaturation: clean the clean cowhide obtained in step S1 cleaning until the pH is neutral, then cut and grind to make cowhide slurry, add the cowhide slurry to 6 times the mass fraction of water of the cowhide slurry, and set the heat preservation temperature during the denaturation to 80°C, the holding time is set to 4h, and the denatured cowhide protein slurry is obtained;

[0060] S3 Enzymatic hydrolysis: Add 5 parts of alkaline protease to the denatured cowhide protein slurry obtained in the denaturation of step S2, keep the temperature at 50 °C, keep the pH at 9.5, and react for 5 h. During the enzymatic hydrolysis process, ultrasonic-assisted enzymatic hyd...

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Abstract

The invention belongs to the technical field of enzymatic hydrolysis of bovine collagen peptides, and in particular relates to a production process of bovine collagen peptides, including S1 cleaning, S2 denaturation, S3 enzymatic hydrolysis, S4 centrifugation, S5 enzyme inactivation, S6 impurity removal, and S7 finished product. The present invention has the following advantages and effects: the application uses ultrasonic technology, denaturation technology and enzymatic hydrolysis technology to work together, which can be conveniently adapted to cowhide from various origins; and the collagen peptide product produced by the technology provided by the application has good dissolution properties and dispersibility, good sensory quality, high yield, and a large amount of active polypeptides in the product make it have strong antioxidant capacity; the method of this application has relatively mild reaction conditions, low production energy consumption, and far superior process conditions and process effects in existing technical solutions.

Description

technical field [0001] The invention belongs to the technical field of enzymatic hydrolysis of bovine collagen peptides, and particularly relates to a production process of bovine bovine collagen peptides. Background technique [0002] Collagen peptides are obtained by hydrolysis of collagen. Due to the smaller molecular weight, the increase of polar groups increases the solubility. After the collagen is degraded into collagen peptides, due to the leakage of a large number of hydrophilic groups, the water absorption and water holding capacity of collagen peptides are significantly higher than those of collagen. Using it in cosmetics can maintain skin moisture and improve skin moisturization. Effect. The emulsifying and foaming properties of collagen peptides also make them useful as food additives to improve the quality of food, making the products not only taste good, but also rich in nutrients. In addition to the general functional properties of proteins, collagen peptid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/34
CPCC12P21/06C07K14/78
Inventor 尹晓清吴海靳祯亮王希搏吴梦婷王微
Owner 湖北瑞邦生物科技有限公司
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