Malic enzyme (NADP-ME) gene and application thereof

A malic enzyme and gene technology, applied in the field of molecular biology, can solve problems such as chloroplast growth deformity, and achieve the effect of improving photosynthetic efficiency

Active Publication Date: 2014-03-05
HENAN ACAD OF AGRI SCI XIAOMAI INST
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Takeuchi et al. (2000) transferred corn nadp-me into rice, and the NADP-ME enzyme activity in the leaves of the transgenic plants increased by 20-70 times, but the chloroplasts of the transgenic plants grew abnormally, and there were no thylakoid membranes stacked together grana

Method used

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  • Malic enzyme (NADP-ME) gene and application thereof
  • Malic enzyme (NADP-ME) gene and application thereof
  • Malic enzyme (NADP-ME) gene and application thereof

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Embodiment 1

[0018] 1. Construction of high light efficiency nadp-me gene expression vector

[0019] The corn inbred line material involved in the present invention is provided by the Grain Crops Research Institute of the Henan Academy of Agricultural Sciences or the corn inbred line material generally used in Henan. Experimental RNA extraction kits and agarose gel DNA recovery kits were purchased from Tianwei Times Company, MLV reverse transcription kits were purchased from Promega Company, LA-Taq DNA polymerase, pMD-19T cloning vector, Escherichia coli (Escherichia coli) strain DH5α competent cell preparation kits are all products of TaKaRa Company, the plant expression vector pCOMBIA3301 is preserved in our laboratory, the restriction endonuclease is the product of MBI Company, the ampicillin is the product of Amersco Company, and the rest of the reagents are imported or domestic analysis pure.

[0020] According to the reported maize nadp-me gene cDNA sequence (GenBank accession numbe...

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Abstract

The invention discloses a malic enzyme (NADP-ME) gene and application thereof. The novel NADP-ME gene provided by the invention can be stably expressed and inherited in wheat, thus obviously improving photosynthetic efficiency compared with an acceptor material. Therefore, the NADP-ME gene provides a significant gene source and parent material support for improving photosynthetic efficiencies of C3 crops through a C4 high-photosynthetic efficiency gene and for selecting a high-photosynthetic efficiency transgenic wheat variety which substantially improves yield level.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a malic acid enzyme (NADP-ME) gene and application thereof. Background technique [0002] Wheat is the main food crop in my country, and its production capacity and supply and demand are always related to major strategic issues such as my country's national economic development and food security. From a global perspective, the state of world grain production has always been closely related to the political and economic development of each country. Once grain production and reserves decline, international grain prices will rise sharply, and panic will spread. From a domestic point of view, with the growth of my country's population, the rapid development of industrialization and urbanization, the demand for food will continue to increase. At the same time, since the acreage of grain crops in my country cannot recover significantly, the only way to ensure my country's food security...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/04C12N15/82A01H5/00
Inventor 许为钢王玉民张磊王会伟李艳胡琳齐学礼董海滨
Owner HENAN ACAD OF AGRI SCI XIAOMAI INST
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