A kind of method of synthesizing phenylalanyl-lysine dipeptide

A technology of lysine dipeptide and phenylalanyl, which is applied in the field of peptide synthesis to achieve mild conditions, great social value and economic benefits, and high synthesis efficiency

Inactive Publication Date: 2021-01-29
吴?基
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to address the shortcomings of the existing method for enzymatically synthesizing short peptides in non-aqueous media, and to provide a method for synthesizing phenylalanyl-lysine dipeptides using biological enzyme catalysts in ethylene glycol, an organic medium. method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for synthesizing phenylalanyl-lysine dipeptide, its specific process steps are as follows:

[0034] (1) Preparation of phenylalanine and lysine aqueous solution

[0035] Dissolve an appropriate amount of commercially available phenylalanine and lysine in a pure aqueous solution successively under stirring to prepare an aqueous solution with a phenylalanine concentration of 0.3mol / L and a lysine concentration of 0.3mol / L; Sodium hydroxide adjusts the pH value of the aqueous solution to 7.5, that is, an aqueous solution of phenylalanine and lysine is prepared, which is used in the next step to prepare an ethylene glycol non-aqueous reaction medium.

[0036] (2) Preparation of ethylene glycol non-aqueous reaction medium

[0037] After the (1) step was completed, the phenylalanine and lysine aqueous solution prepared in the (1) step were mixed with ethylene glycol solution, wherein the volume ratio of phenylalanine and lysine aqueous solution to ethylene glycol 1...

Embodiment 2

[0051] A method for synthesizing phenylalanyl-lysine dipeptide, its specific process steps are as follows:

[0052] (1) Preparation of phenylalanine and lysine aqueous solution

[0053] Dissolve an appropriate amount of commercially available phenylalanine and lysine in a pure aqueous solution successively under stirring to prepare an aqueous solution with a concentration of phenylalanine of 0.6mol / L and a concentration of lysine of 1.2mol / L; Sodium hydroxide adjusts the pH value of the aqueous solution to 8.5, that is, an aqueous solution of phenylalanine and lysine is prepared, which is used in the next step to prepare an ethylene glycol non-aqueous reaction medium.

[0054] (2) Preparation of ethylene glycol non-aqueous reaction medium

[0055] After the (1) step was completed, the phenylalanine and lysine aqueous solution prepared in the (1) step were mixed with ethylene glycol solution, wherein the volume ratio of phenylalanine and lysine aqueous solution to ethylene gly...

Embodiment 3

[0069] A method for synthesizing phenylalanyl-lysine dipeptide, its specific process steps are as follows:

[0070] (1) Preparation of phenylalanine and lysine aqueous solution

[0071] Dissolve an appropriate amount of commercially available phenylalanine and lysine in a pure aqueous solution successively under stirring to prepare an aqueous solution with a phenylalanine concentration of 1.0mol / L and a lysine concentration of 2.0mol / L; Sodium hydroxide adjusts the pH value of the aqueous solution to 9.5, that is, an aqueous solution of phenylalanine and lysine is prepared, which is used in the next step to prepare an ethylene glycol non-aqueous reaction medium.

[0072] (2) Preparation of ethylene glycol non-aqueous reaction medium

[0073] After the (1) step was completed, the phenylalanine and lysine aqueous solution prepared in the (1) step were mixed with ethylene glycol solution, wherein the volume ratio of phenylalanine and lysine aqueous solution to ethylene glycol 1...

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Abstract

A method for synthesizing phenylalanyl-lysine dipeptide belongs to the technical field of peptide synthesis. The invention uses commercially available phenylalanine and lysine as raw materials, prepares ethylene glycol non-aqueous reaction medium by preparing phenylalanine and lysine aqueous solution, and prepares phenylalanyl-lysine dipeptide reaction liquid , prepare a resin column for purifying loaded phenylalanyl-lysine dipeptide, prepare phenylalanyl-lysine dipeptide eluate, prepare phenylalanyl-lysine dipeptide freeze-dried powder, prepare recovery The phenylalanine and lysine aqueous solution and the step of preparing the regenerated resin column just prepare the phenylalanyl-lysine dipeptide with a content of 95-98%. The present invention uses chymotrypsin as a catalyst to synthesize phenylalanyl-lysine dipeptide in an ethylene glycol non-aqueous medium, the conditions are mild, the specificity is strong, and the synthesis efficiency is high, which is a typical green production process; the prepared Phenylalanyl-lysine dipeptide can be widely used in medicine, textile, food and other fields, and has considerable social value.

Description

[0001] 1. Technical field [0002] The invention belongs to the technical field of peptide synthesis, and in particular relates to a method for synthesizing phenylalanyl-lysine dipeptide. [0003] 2. Background technology [0004] Short peptides are chain compounds composed of 2-9 amino acid residues. Many short peptides have important biological functions and are new members of biomedicine. They are also widely used in food, health products and daily chemicals. middle. For example, aspartyl-phenylalanine dipeptide is an important peptide sweetener, which is widely used in food, cosmetics and other fields; The decomposition of phenylalanine and lysine, phenylalanine is one of the essential amino acids, is the intermediate for the synthesis of amino acid anticancer drugs such as phenylalanine and formic acid sarcoma, and is also used for the production of adrenaline and thyroxine In the field of food, phenylalanine is one of the raw materials for the synthesis of health-care d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/065C12P21/02C07K1/36C07K1/34C07K1/18C07K1/14
CPCC07K5/06078C12P21/02
Inventor 尹思力朱云阳张海龙江文杨洋倪科凌梦晨周小华
Owner 吴?基
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