The invention relates to a purifying method for terlipressin. The method is characterized by comprising the following steps: step 1, dissolving a crude terlipressin product with water and adjusting a pH value to 3 to 4; step 2, flushing a C18 reversed-phase filled column by using an ion pair mobile phase A containing surfactant; step 3, loading a solution obtained in the step 1 into a C18 reversed-phase filling material; step 4, carrying out gradient procedures, wherein the initial-state mobile phase B of an elution gradient is 5% which is maintained for 5 min, then the proportion of the mobile phase B is increased to 30% within 60 min, and a part of target peak elution fraction is collected; and step 5, carrying out nanofiltration membrane desalination and then freeze drying so as to obtain pure terlipressin.