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Magnetic nanoparticles and application thereof in preparing magnetic solid-phase carriers

A technology of magnetic nanoparticles and solid-phase carriers, which is applied in the preparation method of peptides, the manufacture of inductors/transformers/magnets, electrical components, etc., and can solve problems that affect the performance of peptides, easy aggregation of magnetic particles, and insufficient surface hydroxyl groups

Active Publication Date: 2018-08-07
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the size effect and magnetic dipole attraction of the existing magnetic nanoparticles, the magnetic particles are easy to agglomerate, and the chemical stability is not high, and they are easily oxidized, resulting in insufficient surface hydroxyl groups, so that it is difficult to apply directly. Affects the performance of the synthesized peptide

Method used

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  • Magnetic nanoparticles and application thereof in preparing magnetic solid-phase carriers
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  • Magnetic nanoparticles and application thereof in preparing magnetic solid-phase carriers

Examples

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Embodiment 1

[0035] The synthetic method of magnetic nanoparticle described in the present embodiment, comprises the following steps:

[0036] (1) 12g FeCl 3 ·6H 2 O and 4.9 g FeCl 2 4H 2 O was dissolved in 50mL deionized water, and then at 80°C under N 2In the atmosphere, quickly add 50ml of ammonia water to the above solution under vigorous stirring, and mix well;

[0037] After stirring for 30 minutes, 2 g of oleic acid was added to the above mixture, and the suspension was kept at 80° C. for 1 hour. After the reaction, the magnetic nanoparticles were washed with deionized water until neutral, and vacuum-dried to obtain oleic acid-modified Superparamagnetic Fe 3 o 4 particle;

[0038] The obtained oleic acid-modified superparamagnetic Fe 3 o 4 Particles were subjected to microscopic morphology analysis, and the results were as follows: figure 1 As can be seen from the figure, the superparamagnetic Fe modified by oleic acid 3 o 4 The particles have good monodispersity, unifor...

Embodiment 2

[0044] The synthetic method of magnetic nanoparticle described in the present embodiment, comprises the following steps:

[0045] (1) 12g FeCl 3 ·6H 2 O and 4.9 g FeCl 2 4H 2 O was dissolved in 50mL of deionized water, then at 70°C under N 2 In the atmosphere, quickly add 50mL ammonia water to the above solution under vigorous stirring, and mix well;

[0046] After stirring for 20 minutes, 2 g of oleic acid was added to the above mixture, and the suspension was kept at 70°C for 2 hours. After the reaction, the magnetic nanoparticles were washed with deionized water until neutral, and vacuum-dried to obtain oleic acid-modified Superparamagnetic Fe 3 o 4 particle;

[0047] (2) the obtained oleic acid modified superparamagnetic Fe 3 o 4 The particles are dispersed into n-hexane to form a magnetic fluid (the solid-to-liquid ratio of the magnetic fluid and solvent is controlled to be 0.125g / mL), and styrene, 4-(4-vinylbenzyl oxybenzyl alcohol) and divinylbenzene (DVB) (mo...

Embodiment 3

[0051] The synthetic method of magnetic nanoparticle described in the present embodiment, comprises the following steps:

[0052] (1) 12g FeCl 3 ·6H 2 O and 4.9 g FeCl 2 4H 2 O was dissolved in 50mL deionized water, and then at 80°C under N 2 In the atmosphere, quickly add 50mL ammonia water to the above solution under vigorous stirring, and mix well;

[0053] After stirring for 30 minutes, 2 g of oleic acid was added to the above mixture, and the suspension was kept at 80° C. for 2 hours. After the reaction, the magnetic nanoparticles were washed with deionized water until neutral, and vacuum-dried to obtain oleic acid-modified Superparamagnetic Fe 3 o 4 particle;

[0054] (2) the obtained oleic acid modified superparamagnetic Fe 3 o 4 The particles are dispersed into n-hexane to form a magnetic fluid (the solid-to-liquid ratio of the magnetic fluid and solvent is controlled to be 0.125g / mL), and styrene, 4-(4-vinylbenzyl Oxybenzyl alcohol) and divinylbenzene (DVB) ...

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Abstract

The invention belongs to the technical field of solid-phase peptide synthesis, particularly relates to magnetic nanoparticles for solid-phase peptide synthesis, and further discloses a synthesis method of the magnetic nanoparticles and application of the magnetic nanoparticles in preparing magnetic solid-phase carriers. The magnetic nanoparticles are synthesized by subjecting superparamagnetic ferroferric oxide (Fe3O4) particles which are taken as a magnetic core to oleic acid modification and covering the surface of the magnetic core with polystyrene (PS) to form the magnetic nanoparticles which are of core-shell type PS@Fe3O4 structure; further, surface polymer modified magnetic nano-microspheres are obtained by introducing 1-(4-vinyl benzyloxybenzyl alcohol) in the process of covering Fe3O3 with PS. The magnetic nanoparticles have suitable size, have surface chemical adaptability capable of being functionalized, have excellent dispersibility and compatibility in media, and can be used as the magnetic solid-phase carriers in solid-phase peptide synthesis.

Description

technical field [0001] The invention belongs to the technical field of solid-phase polypeptide synthesis, and specifically relates to a magnetic nanoparticle for solid-phase polypeptide synthesis, and further discloses its synthesis method and its use for preparing magnetic solid-phase carriers in solid-phase polypeptide synthesis. Background technique [0002] As an organic polymer, polypeptide has attracted more and more attention because of its multiple functions and good biocompatibility, making the chemical synthesis method of polypeptide an important area in the field of organic synthesis. At present, the principle of polypeptide synthesis is mainly based on amino acid as the basic unit, and under the action of a catalyst, the carboxyl group and amino group of the amino acid react to form an amide bond to connect and realize the growth of the polypeptide chain. However, in traditional organic peptide synthesis methods, the connection of carboxyl and amino groups betwee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01F41/02C08F212/08C08F212/36C08F212/32C08F2/44C08K3/22C07K1/06C07K1/04
CPCC07K1/04C07K1/06C08F212/08C08K3/22C08K2003/2275H01F41/0253C08F212/36C08F212/32C08F2/44
Inventor 丛海林于冰汤琦杨霆
Owner QINGDAO UNIV
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