Variant cathelicidin-BF15 of cathelicidin-BF and use thereof

A kind of antimicrobial peptide and variant technology of golden krait, which is applied in the field of biomedicine, can solve the problems of unreported antimicrobial peptide research, and achieve the effect of artificial synthesis of broad-spectrum antibacterial, strong killing effect and strong bactericidal effect

Inactive Publication Date: 2009-03-18
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are a large number of reports on the venom protein of kraitus, various proteases and inhibitors, but there is no report on the antimicrobial peptides in kraitus venom

Method used

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  • Variant cathelicidin-BF15 of cathelicidin-BF and use thereof
  • Variant cathelicidin-BF15 of cathelicidin-BF and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0017] Preparation of cathelicidin-BF15:

[0018] I. Preparation method of cathelicidin-BF15: According to the amino acid sequence of cathelicidin-BF, an antimicrobial peptide of B. kraitus obtained through separation and purification, a mutant cathelicidin-BF15 of cathelicidin-BF was designed by proteomics method. The full sequence was synthesized with an automatic peptide synthesizer (433A, Applied Biosystems), and purified by desalting and HPLC reverse-phase column chromatography.

[0019] II. Molecular weight was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF).

[0020] III. The purity of the purified cathelicidin-BF15 is identified by high-performance liquid chromatography (HPLC), the molecular weight is determined by matrix-assisted laser desorption ioniza...

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Abstract

The invention relates to a variant cathelicidin-BF15 of bungarus fasciatus antibiosis peptide cathelicidin-BF and application thereof. The invention belongs to the field of biomedicine. The variant cathelicidin-BF15 of the bungarus fasciatus antibiosis peptide cathelicidin-BF is a straight chain polypeptide which contains 15 amino acid residues with the molecular weight of 1,939.47Da and an isoelectric point of 12.05. The total sequence of the cathelicidin-BF15 is as follows:valine-lysine-arginine-phenylalanine-lysine-lysine-phenylalanine-phenylalanine-arginine-lysine-leucine-lysine-lysine-serine-valine. The cathelicidin-BF15 which has strong bactericidal action can exterminate a plurality of clinical drug-fast bacteria. In addition, the cathelicidin-BF15 is also characterized by simple structure, convenient synthesis, broad spectrum antibiosis, and the like.

Description

Technical field: [0001] The invention provides a Bungarus fasciatus antibacterial peptide cathelicidin-BF variant cathelicidin-BF15 and its application, belonging to the technical field of biomedicine. Background technique: [0002] Cathelicidins are a family of multifunctional antimicrobial peptides that are currently only found in mammals, birds and fish. Cathelicidin has broad-spectrum antibacterial activity, not only has very strong bactericidal activity against Gram-positive bacteria, Gram-negative bacteria, some fungi and viruses, but also has effects on many clinical drug-resistant bacteria. In addition, cathelicidin also has many other biological activities, such as chemotaxis to various immune cells, inducing mast cell degranulation and histamine release, regulating macrophage transcription, promoting wound healing, inducing angiogenesis, inducing mutation Cell line apoptosis and lymphocyte activation, etc. Because of so many activities, cathelicidin has been a re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/00
Inventor 赖仞王义鹏杨海龙
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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