Pinctada martensii antimicrobial peptide PmAMP and application thereof

Abstract

The invention discloses a pinctada martensii antimicrobial peptide PmAMP and application thereof.The amino sequence of the inctada martensii antimicrobial peptide PmAMP is as shown in SEQ ID No.1.The pinctada martensii antimicrobial peptide PmAMP is characterized in that pinctada martensii is used as the research object, the serum of the pinctada martensii is separated and purified to screen out protein with antimicrobial activity, the obtained pinctada martensii antimicrobial peptide PmAMP can evidently inhibit the growth of escherichia coli and micrococcus luteus, the gene for encoding the antimicrobial peptide PmAMP are obtained through mass spectrum sequencing and using the RACE technology to clone the overall length of cDNA, and the nucleotide sequence of the gene is as shown in SEQ ID No.2 or SEQ ID No.3.When fluorescent quantitative PCR is used to detect the expression content of the PmAMP in tissue, the detection results show that the expression content of the PmAMP is the highest in adductor muscle and low in blood cells.After bacterium stimulation, the expression content of the PmAMP in the blood cells is the highest after 8 hours, so that the PmAMP is immune protein.By the pinctada martensii antimicrobial peptide PmAMP, a foundation is laid for the immunology researches and disease control of the pinctada martensii.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the antimicrobial peptide PmAMP of Pinctada martensii and its application. Background technique [0002] Antimicrobial peptides are natural peptides that do not cause resistance like antibiotics and are found in many marine organisms. When some traditional antibiotics were banned, many scholars turned to the research of antimicrobial peptides and achieved a series of results. Antimicrobial peptides can improve plant disease resistance. People introduce some antimicrobial peptide genes into plants to make plants resistant to certain diseases. In food, some additives will cause harm to people’s bodies. Antimicrobial peptides are applied to Food preservatives can effectively kill some mildew microorganisms without causing harm to the human body. Ling Binxun and others found that the antibacterial peptide of tussah silkworm has anti-tumor effect. It is also very import...

AI Technical Summary

Problems solved by technology

In recent years, due to the deterioration of coastal water quality, various diseases have occurred frequently, resulting in a large number of deaths of Pinctada martensii due to diseases, which has brought serious economic losses to my country's pearl industry. Improving the stress resistance of Pinctada martensii is currently the Urgent problems

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