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Application of Pluronic F-127 solution in chick embryo chorioallantoic membrane experiment

A technology of chorioallantoic membrane and pluronicf-127, which is applied in the direction of material inspection products, testing pharmaceutical preparations, etc., to achieve the effect of reducing temperature-sensitive drug denaturation and low toxicity

Inactive Publication Date: 2013-06-05
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After reviewing the literature, there is no Pluronic F-127 used as a drug delivery carrier in blood vessel research experiments using CAM as a model

Method used

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  • Application of Pluronic F-127 solution in chick embryo chorioallantoic membrane experiment
  • Application of Pluronic F-127 solution in chick embryo chorioallantoic membrane experiment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] In an environment at 4°C, take 20 μL of Pluronic F-127 solution with a concentration of 20% (w / v), and slowly add it to the surface of the CAM. Chicken embryos were further cultured at 38.5°C and 85% relative humidity for 48 hours, and then the results were recorded with a stereo microscope. The CAM grew normally, without deformation, and there was no change in vessel density compared with the surrounding area without Pluronic F-127 solution.

Embodiment 2

[0019] In a 4°C environment, dissolve Lucentis (chemical name: Ranibizumab, a product of Roche, an antivascular drug for the treatment of age-related wet macular degeneration) in the present invention at a concentration of 20% (w / v), so that the final concentration of Lucentis 100 μM. Take 20 μL and slowly add to the surface of the CAM. Chicken embryos were further cultured at 38.5°C and 85% relative humidity for 48 hours, and then the results were recorded with a stereo microscope. The density of blood vessels in the drug-dosed area was significantly reduced.

Embodiment 3

[0021] Pluronic F-127 solution concentration is 25% (w / v) in this invention, all the other are with embodiment 1.

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Abstract

The invention relates to application of Pluronic F-127 solution in chick embryo chorioallantoic membrane experiment as medicine carrying material. The application includes the steps of setting the Pluronic F-127 solution at 4 DEG C through still-setting method to dissolve the solution, configurating biological isotonic solution of certain concentration, and thus the medicine carrying material is obtained. The Pluronic F-127 solution is in liquid state in low temperature, and thus the solution can be mixed with tested medicine conveniently, and possibility of temperature-sensitive medicine degeneration is reduced to the greatest extent. The Pluronic F-127 solution is transparent when the solution is in solid state in high temperature, and thus chick embryo chorioallantoic membrane does not deform when the Pluronic F-127 solution is used and observation is convenient.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of Pluronic F-127 solution as a drug-loading material in chicken embryo chorioallantoic membrane experiments. technical background [0002] Angiogenesis is an important step in tumor development and metastasis. Therefore, in recent years, the mechanism of angiogenesis and the research and development of anti-angiogenesis drugs have become one of the research hotspots in oncology. Chicken chorioallantoic membrane (chick embryo chorioallantoic membrane, CAM) model is a living animal model widely used in anti-angiogenesis research. Not only because of its simple operation and low cost, but also more convenient for observation compared with the mouse model. [0003] When conducting experiments, local administration is generally carried out by placing small pieces of filter paper, plastic rings (usually cut from pipette tips), etc. on the CAM, but these methods have cert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/15
Inventor 史通麟李卓玉张全斌李宗伟赵超单树花
Owner SHANXI UNIV
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