Extraction process of low-molecular-weight fucoidan and application thereof in cosmetics
An extraction process and technology for fucoidan, applied in the field of natural product extraction, can solve the problems of restricting the research and market development progress of fucoidan, low molecular weight of fucoidan, poor product fluidity, etc., and can regulate inflammation and allergic reactions. , Inhibit the production of monosaccharides, promote the effect of endogenous collagen synthesis
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Embodiment 1
[0032] A low molecular weight fucoidan extraction process, the specific steps are as follows:
[0033] (1) Grinding and sieving the kelp, extracting with water, centrifuging to obtain the supernatant, and obtaining the extract;
[0034] (2) Freeze the extract at -40°C. After complete freezing, slowly thaw at room temperature (25°C), centrifuge at 0°C, take the first supernatant, and repeat the process of freezing, thawing and centrifuging until no precipitation occurs , to obtain the second supernatant, add α-amylase to remove starch, then add absolute ethanol until its volume concentration is 70%, let stand to get the precipitate, freeze-dry, polysaccharide powder;
[0035] (3) Grinding to a particle size of 100nm to obtain nanopowder;
[0036] (4) Add the nano-powder into water, stir well until it is evenly dispersed, add ferric chloride while stirring to a final concentration of 0.003mol / L, first irradiate with gamma rays, then irradiate with electron beams, concentrate an...
Embodiment 2
[0049] A low molecular weight fucoidan extraction process, the specific steps are as follows:
[0050] (1) Grinding and sieving the kelp, extracting with water, centrifuging to obtain the supernatant, and obtaining the extract;
[0051] (2) Freeze the extract at -50°C. After complete freezing, slowly thaw at room temperature (25°C), centrifuge at 4°C, take the first supernatant, and repeat the process of freezing, thawing and centrifuging until no precipitation occurs , to obtain the second supernatant, add α-amylase to remove starch, then add absolute ethanol until its volume concentration is 80%, let stand to get the precipitate, freeze-dry, polysaccharide powder;
[0052] (3) Grinding to a particle size of 200nm to obtain nanopowder;
[0053] (4) Add the nano-powder into water, stir well until it is evenly dispersed, add ferric chloride while stirring to a final concentration of 0.005mol / L, first irradiate with gamma rays, then irradiate with electron beams, concentrate an...
Embodiment 3
[0066] A low molecular weight fucoidan extraction process, the specific steps are as follows:
[0067] (1) Grinding and sieving the kelp, extracting with water, centrifuging to obtain the supernatant, and obtaining the extract;
[0068] (2) Freeze the extract at -40°C. After freezing completely, slowly thaw at room temperature (25°C), centrifuge at 4°C, take the first supernatant, and repeat the process of freezing, thawing and centrifuging until no precipitation occurs , to obtain the second supernatant, add α-amylase to remove starch, then add absolute ethanol until its volume concentration is 70%, let stand to get the precipitate, freeze-dry, polysaccharide powder;
[0069] (3) Grinding to a particle size of 200nm to obtain nanopowder;
[0070] (4) Add the nano-powder into water, stir well until it is evenly dispersed, add ferric chloride while stirring to a final concentration of 0.003mol / L, first irradiate with gamma rays, then irradiate with electron beams, concentrate ...
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