Extraction process of low-molecular-weight fucoidan and application thereof in cosmetics

An extraction process and technology for fucoidan, applied in the field of natural product extraction, can solve the problems of restricting the research and market development progress of fucoidan, low molecular weight of fucoidan, poor product fluidity, etc., and can regulate inflammation and allergic reactions. , Inhibit the production of monosaccharides, promote the effect of endogenous collagen synthesis

Inactive Publication Date: 2019-05-03
金耀仑
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, limited by the extraction technology, most of the fucoidan produced today are macromolecular heteropolysaccharides with complex components and unclear structure, which limits the progress of fucoidan research and market development
Patent CN106866834B discloses a method ...

Method used

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Examples

Experimental program
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Embodiment 1

[0032] A low molecular weight fucoidan extraction process, the specific steps are as follows:

[0033] (1) Grinding and sieving the kelp, extracting with water, centrifuging to obtain the supernatant, and obtaining the extract;

[0034] (2) Freeze the extract at -40°C. After complete freezing, slowly thaw at room temperature (25°C), centrifuge at 0°C, take the first supernatant, and repeat the process of freezing, thawing and centrifuging until no precipitation occurs , to obtain the second supernatant, add α-amylase to remove starch, then add absolute ethanol until its volume concentration is 70%, let stand to get the precipitate, freeze-dry, polysaccharide powder;

[0035] (3) Grinding to a particle size of 100nm to obtain nanopowder;

[0036] (4) Add the nano-powder into water, stir well until it is evenly dispersed, add ferric chloride while stirring to a final concentration of 0.003mol / L, first irradiate with gamma rays, then irradiate with electron beams, concentrate an...

Embodiment 2

[0049] A low molecular weight fucoidan extraction process, the specific steps are as follows:

[0050] (1) Grinding and sieving the kelp, extracting with water, centrifuging to obtain the supernatant, and obtaining the extract;

[0051] (2) Freeze the extract at -50°C. After complete freezing, slowly thaw at room temperature (25°C), centrifuge at 4°C, take the first supernatant, and repeat the process of freezing, thawing and centrifuging until no precipitation occurs , to obtain the second supernatant, add α-amylase to remove starch, then add absolute ethanol until its volume concentration is 80%, let stand to get the precipitate, freeze-dry, polysaccharide powder;

[0052] (3) Grinding to a particle size of 200nm to obtain nanopowder;

[0053] (4) Add the nano-powder into water, stir well until it is evenly dispersed, add ferric chloride while stirring to a final concentration of 0.005mol / L, first irradiate with gamma rays, then irradiate with electron beams, concentrate an...

Embodiment 3

[0066] A low molecular weight fucoidan extraction process, the specific steps are as follows:

[0067] (1) Grinding and sieving the kelp, extracting with water, centrifuging to obtain the supernatant, and obtaining the extract;

[0068] (2) Freeze the extract at -40°C. After freezing completely, slowly thaw at room temperature (25°C), centrifuge at 4°C, take the first supernatant, and repeat the process of freezing, thawing and centrifuging until no precipitation occurs , to obtain the second supernatant, add α-amylase to remove starch, then add absolute ethanol until its volume concentration is 70%, let stand to get the precipitate, freeze-dry, polysaccharide powder;

[0069] (3) Grinding to a particle size of 200nm to obtain nanopowder;

[0070] (4) Add the nano-powder into water, stir well until it is evenly dispersed, add ferric chloride while stirring to a final concentration of 0.003mol / L, first irradiate with gamma rays, then irradiate with electron beams, concentrate ...

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Abstract

The invention provides an extraction process of low-molecular-weight fucoidan, and the obtained product is low in molecular weight (400-500Da), is high in purity and has proper good liquidity. The extraction process comprises the following steps: firstly, extracting kelp with water to prepare a leaching solution, carrying out repeated freeze-thawing treatment on the leaching solution, using alpha-amylase to remove starch, carrying out an alcohol precipitation step to obtain polysaccharide powder, crushing the polysaccharide powder into nano powder, then redissolving the nano powder in water, and carrying out coupling degradation by virtue of ferric trichloride and irradiation of gamma rays and electron beams to obtain the low-molecular-weight fucoidan.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to an extraction process of low molecular weight fucoidan and its application in cosmetics. Background technique [0002] In recent years, small-molecule fucoidan has received intense attention, especially products with a molecular weight of about 500 Da, whose activity is more than 40% higher than that of ordinary fucoidan. [0003] Fucoidan is a natural complex polysaccharide, which contains a natural sulfate group at the end of the molecular chain, which makes it exhibit excellent and obvious physiological activities. Modern science has proved that fucoidan is not a compound with a single structure, but a family of compounds with different chemical components. The general molecular weight of fucoidan is about 20000 Da, which has antiviral, immune regulation and anticancer effects. The content of fucoidan in kelp is about 1-2%. [0004] At present, the effica...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K8/73A61Q19/00
Inventor 叶秀清金耀仑
Owner 金耀仑
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