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Separation and preparation method of deer serum

A serum and centrifuge bottle technology, which is applied in biochemical equipment and methods, microorganisms, culture processes, etc., can solve problems such as high price and achieve the effects of low IgG concentration and glucose content, and a simple and fast preparation method.

Inactive Publication Date: 2016-06-08
苏州红冠庄药物研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since most of the serum medium used for precious cell culture is imported from abroad, the price is relatively expensive, causing a large cost burden to the user

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0024] (1) Blood collection: The above-mentioned selected deer to be blooded is sequentially cleaned and disinfected with iodine with a concentration of 2wt.% and alcohol with a concentration of 75vol.%. Whole blood was collected from the veins of the deer body after cleaning and disinfection, and the collected whole blood was stored in a centrifuge bottle and allowed to stand at 4°C for 6 hours;

[0025] (2) Separation: Centrifuge the deer whole blood treated in (1) at a speed of 2000rpm for 50min at 4°C. After the centrifugation, extract the serum from the centrifuge bottle in a vacuum environment. Store at -25°C for later use to obtain frozen serum;

[0026] (3) Fibrin removal: Thaw the frozen serum obtained from (2) in a refrigerator at 4°C, filter the thawed serum through eight layers of sterile gauze to remove fibrin, and collect the filtrate;

[0027] (4) Inactivation: place the filtrate obtained after the treatment in (3) in a constant temperature water bath for 40 mi...

specific Embodiment 2

[0031] (1) Blood collection: The above-mentioned selected deer to be blooded is sequentially cleaned and disinfected with iodine with a concentration of 2wt.% and alcohol with a concentration of 75vol.%. Whole blood was collected from the veins of the deer body after cleaning and disinfection, and the collected whole blood was stored in a centrifuge bottle and allowed to stand at 5°C for 5 hours;

[0032] (2) Separation: Centrifuge the deer whole blood treated in (1) at a speed of 3000rpm for 40min at 5°C. After centrifugation, extract the serum from the centrifuge bottle in a vacuum environment. Store at -30°C for later use to obtain frozen serum;

[0033] (3) Fibrin removal: Thaw the frozen serum obtained from (2) in a refrigerator at 5°C, filter the thawed serum through eight layers of sterile gauze to remove fibrin, and collect the filtrate;

[0034] (4) Inactivation: place the filtrate obtained after the treatment in (3) in a constant temperature water bath for 30 minute...

specific Embodiment 3

[0038] (1) Blood collection: The above-mentioned selected deer to be blooded is sequentially cleaned and disinfected with iodine with a concentration of 2wt.% and alcohol with a concentration of 75vol.%. Whole blood was collected from the veins of the deer body after cleaning and disinfection, and the collected whole blood was stored in a centrifuge bottle and allowed to stand at 4°C for 5 hours;

[0039] (2) Separation: Centrifuge the deer whole blood treated in (1) at a speed of 2500rpm for 45min at 4°C. After the centrifugation, extract the serum from the centrifuge bottle in a vacuum environment. Store at -25°C for later use to obtain frozen serum;

[0040] (3) Fibrin removal: Thaw the frozen serum obtained from (2) in a refrigerator at 4°C, filter the thawed serum through eight layers of sterile gauze to remove fibrin, and collect the filtrate;

[0041] (4) Inactivation: place the filtrate obtained after the treatment in (3) in a constant temperature water bath for 35 mi...

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Abstract

The invention discloses a separation and preparation method of deer serum. According to the method, the deer whole blood obtained after sterile blood sampling from a cleaned and disinfected to-be-sampled deer is left to stand and subjected to centrifugal separation, fibrous protein is removed, then the whole blood is inactivated, the concentration of IgG and the content of glucose are reduced, obtained serum is irradiated with a certain dosage of gamma-rays, viruses and mycoplasmas are removed, the serum is irradiated with ultraviolet rays for sterilization while being filtered by an asbestos-free Chua's filter disc, deer serum filtrate is obtained, finally, the deer serum filtrate is filtered with a filtering membrane, and a finished product, the deer serum, is obtained. The preparation method is simple, convenient, quick, stable in process and suitable for large-scale production; the obtained deer serum does not contain fibrous protein, the concentration of IgG and the content of glucose are low, pollution of the viruses, germs and mycoplasmas can be completely eliminated, and the prepared deer serum can be used for preparation of a culture medium containing serum cells.

Description

technical field [0001] The invention relates to a production process of deer serum, in particular to a separation and preparation method of deer serum. Background technique [0002] At present, cell culture technology and methods have been widely used in medicine, medical treatment, bioengineering, genetic engineering, vaccine production and other fields, among which cell culture fluid has become an irreplaceable biological material in the production and research of biotechnology products. Currently, the cell culture fluids circulating in the domestic market are mainly cell culture fluids containing calf serum or fetal bovine serum, and some serum-free cell culture fluids. Most of the bovine serum used comes from Australia, New Zealand and other countries, while domestic sources of bovine serum are mainly concentrated at the bottom of the market, and most of the raw materials come from slaughtered cattle blood. [0003] After the mad cow disease incident in the world in rec...

Claims

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Application Information

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IPC IPC(8): C12N5/00
CPCC12N5/0018C12N2500/84
Inventor 党平宋平陈建兵杜雨威王瑜
Owner 苏州红冠庄药物研究有限公司
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