Preparation method of bone repair hydrogel and fiber pipe composite material loading growth factors

Abstract

The invention discloses a preparation method of a bone repair hydrogel and fiber pipe composite material loading growth factors. Firstly, a spinning solution is prepared, the spinning solution is arranged in a syringe, a nanometer fiber reticular layer is prepared by high-voltage electrospinning, sodium alginate modified by RGD (Arg-Gly-Asp) is irradiated with gamma rays, and after irradiation, the RGD-sodium alginate is filtered to be degermed and is stored at the room temperature of -20 DEG C so as to prepare hydrogel. According to the method disclosed by the invention, the sodium alginate degraded by gamma ray irradiation can be combined with adhesive peptide in covalence to promote cell attachment; a nanometer fiber pipe is prepared through electrostatic spinning, the fiber has a diameter being the same as that of an extracellular matrix (ECM) and a large specific surface area, can improve cell attachment and migration, and is suitable for osteoblast differentiation and stem cell in vitro regeneration; and the composite material consisting of the bone repair hydrogel and the fiber pipe can guide bone regeneration by hydrogel injected with the sodium alginate and the protein electrostatic spinning nanometer fiber reticular film, promotes the regeneration and repair functions of vessels, can be used as a bone repair transmission system, has a simple production process and is easy to operate.

Description

Technical field: [0001] The invention relates to a biological composite material, in particular to a preparation method of a bone repair hydrogel and fiber tube composite material loaded with growth factors. Background technique: [0002] Autograft and allograft bone grafting are the most conventional ways to treat fractures and extensive bone defects, however, this technique has many drawbacks, including limited availability of bone graft materials, high graft failure rate, etc. Through tissue engineering / regenerative medicine (TE / RM), some potential bone graft substitutes have been developed, combining biochemical and physical scaffolding structures, using three-dimensional scaffolds to support in vivo loading, however, these scaffolding structures are difficult to design and fabricate With high porosity products, they often fail to provide an optimal environment for damaged cells, thereby hindering the restoration of damaged tissue function. [0003] Electrospun nanofibr...

AI Technical Summary

Problems solved by technology

[0002] Autologous and allogeneic bone grafts are the most conventional means of treating fractures and extensive bone defects, however, this technique has many drawbacks, including limited availability of bone graft materials, high graft failure rate, etc.

Through tissue engineering / regenerative medicine (TE / RM), some potential bone graft substitutes have been developed, combining biochemical and physical scaffolding structures, using three-dimensional scaffolds to support in vivo loading, however, these scaffolding structures are difficult to design and fabricate Products with high porosity, they usually cannot provide an optimal environment for damaged cells, thus hindering the recovery of damaged tissue function