Culture method for largely amplifying hair follicle stem cells in vitro

A hair follicle stem cell and cell culture technology, which is applied in the field of bioreactors, can solve the problems of loss of differentiation potential and decreased proliferation ability, and achieve the effects of avoiding the weakening of cell proliferation ability, easy operation and strong proliferation ability.

Inactive Publication Date: 2010-12-15
JILIN UNIV
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AI Technical Summary

Problems solved by technology

However, after repeated passage of cells, not only the ability to proliferate rapidly, but also lose the potential to differentiate into a variety of tissue-specific cells, and may even induce tumor formation after transplantation

Method used

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Embodiment Construction

[0022] The present invention comprises the following steps:

[0023] 1. Preparation of equipment and materials, including:

[0024] ①Cell culture spinner bottle: vertical spinner bottle and horizontal spinner bottle, with a capacity of 10-10000ml and a speed of 1-500 rpm;

[0025] ②Microcarrier: its material is composed of one or more biodegradable materials, or composed of non-biodegradable materials, or composed of biodegradable and non-biodegradable materials;

[0026] The biodegradable material is collagen, blood fibrinolytic protein, gelatin, hyaluronic acid, polylactic acid, polyglycolic acid, polyurethane, chitosan; the non-biodegradable material is glass, silica gel, metal ,plastic;

[0027] ③ Cells: including mammalian hair follicle stem cells and human hair follicle stem cells;

[0028] ④ Culture medium: including DMEM, DMM-F12, EGM, KGM, IMDM, MEM, EBM, in which one or more than two biological factors are added;

[0029] Biological factors are IGF, bFGF, EGF, VE...

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Abstract

The invention discloses a culture method for largely amplifying hair follicle stem cells in vitro, which belongs to the technical field of cell culture. The invention establishes a method for largely amplifying hair follicle stem cells in vitro, which uses microspheres as carriers for cell culture and a rotary bottle for cell proliferation fermentation tank. The method is simple and convenient in operation, economic and practical, avoids reduction in cell proliferation capacity of by repeated passage cells, differentiation potential and the like and related side effects of the conventional cell amplification method and saves culture solution at the same time; the hair follicle stem cells cultured by the method have high multiplication capacity and retain original differentiation potential and can be used for: (1) building hair follicle stem cell bank for providing high-quality seed cells for study on adult stem cells; (2) transplanting and repairing pathological tissue organs; (3) constructing tissue-engineered organs which are used as substitutes for autologous organs for transplanting and repairing pathological or loss tissue organs; and (4) serving as target cells in genetic treatment for treating corresponding diseases.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and specifically relates to a method for rapidly amplifying and growing adherent cells in large quantities in vitro, in particular to a biological method for amplifying hair follicle stem cells in large quantities in vitro by using microspheres as carriers for cell culture and rotating bottles as fermenters. Reactor technology. Background technique [0002] Disease poses a major threat to human health and survival, and is the most important social problem faced by countries all over the world. The advent and progress of stem cell transplantation technology has brought new vitality and hope to patients whose diseases are considered "incurable" by traditional treatment methods. However, the source of stem cells, the safety and effectiveness of transplantation therapy have always been major scientific issues that need to be resolved in the research of stem cell regenerative medicine. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0666C12N2531/00
Inventor 刘晋宇李玉林
Owner JILIN UNIV
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