Preparation method of hair follicle stem cells

Abstract

The invention discloses a preparation method of hair follicle stem cells. The method comprises the following steps: cutting roots of hair follicles of volunteers, performing cleaning, adding 0.25% Dispase enzyme, and performing digesting at 37 DEG C for 0.5-1.5 hours; after enzyme digestion, and inoculating the obtained material into a hair follicle stem cell culture medium, wherein the culture conditions are that the temperature is 37 DEG C, the CO2 content is 5 percent, and a liquid is changed once every 1 to 2 days; subjecting hair follicle stem cells to amplification: transferring the hairfollicle stem cells obtained in the step S2 into a hair follicle stem cell amplification culture medium for continuous culture, wherein the hair follicle stem cell amplification culture medium comprises the following components: a DMEM basal culture medium, FBS, T-beta, hEGF, VEGF and Rho related kinase; and subjecting the hair follicle stem cells to purification. The hair follicle stem cells prepared by the method disclosed by the invention can be subjected to long-term in-vitro subculture, have very high regeneration and differentiation capacities, lay a foundation for being applied to treatment of skin defects, and have a very good clinical application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing hair follicle stem cells. Background technique [0002] As the largest organ of the human body, the skin has multiple functions such as sensation, temperature regulation, secretion and excretion, and prevention of water evaporation. The most important function is to serve as a barrier between the human body and the external environment to maintain the stability of the internal environment. important parts of. At present, there are more and more traumas involving skin defects, especially burns, crush injuries, and cut injuries. Among them, large-area skin defects caused by trauma often lead to very serious physical disabilities and even death. Currently, the standard treatment for skin defects is autologous skin transplantation. Due to its characteristics of no immune rejection and high survival rate, it is widely used clinically and has achieved good curative...

AI Technical Summary

Problems solved by technology

However, the disadvantages of autologous skin transplantation are also very obvious. Since it is an autologous material, it is a re-injury to the patient, which greatly increases the pain of the patient, and there is a risk of complications such as infection of the donor site and non-healing.

What's more serious is that for the above-mentioned large-area skin defects, it is often difficult to repair the wound due to the lack of enough autologous skin for transplantation, which seriously affects the treatment process and even leads to death.

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