Culture method of hair follicle stem cell for treating baldness, as well as syringe

A hair follicle stem cell and culture method technology, which is applied in the field of hair follicle stem cell culture method and syringe for the treatment of alopecia, can solve problems such as complicated operation process, many instruments used, and limitation of umbilical cord blood, and achieve obvious treatment, convenient operation, and obvious effect Effect

Inactive Publication Date: 2012-07-18
北京雍禾植发技术研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, stem cells are isolated from umbilical cord blood and injected into bald areas after isolation and culture. However, the source of umbilical cord blood directly limits the application of this technology
[0003] In addition, in the process of using umbilical cord blood to separate stem cells, it is necessary to collect umbilical cord blood stem cells with the same or similar histocompatibility genes as the patient, and immune rejection must be considered; the operation process is complicated, many instruments are used, and the cost is large

Method used

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  • Culture method of hair follicle stem cell for treating baldness, as well as syringe
  • Culture method of hair follicle stem cell for treating baldness, as well as syringe
  • Culture method of hair follicle stem cell for treating baldness, as well as syringe

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Embodiment Construction

[0057] The technical solutions of the various embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0058] The invention provides a hair follicle stem cell culture method for treating alopecia, such as figure 1 , as shown, includes:

[0059] See figure 1 , A, the isolated hair follicles were digested with separase;

[0060] See figure 1 , B, cutting out the hair follicle bulge of the hair follicle obtained in step A;

[0061] See figure 1 , C, using trypsin to digest the hair follicle bulge obtained in step B;

[0062] See figure 1 , D, centrifuging the hair follicle bulge ...

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Abstract

The invention relates to the field of health-care, and in particular relates to a culture method of hair follicle stem cell for treating baldness, as well as a syringe. The culture method comprises the following steps: A, digesting excised hair follicles by utilizing separase; B, cutting bugle of follicles; C, digesting the follicle bugle by utilizing pancreatin; D, centrifugally collecting the follicle bulge obtained in the step C to obtain cells; E, culturing the cells in DEME (Dulbecco's Modified Eagle's Medium) culture solution, and primarily separating; F, culturing the product which is obtained in primary separation of the step E in a culture dish; and G, centrifugally collecting upper unattached keratinocyte and follicle fibroblast which are cultured in the step F, and culturing to obtain hair follicle stem cells. The syringe comprises a hair follicle stem cell extraction component and a syringe needle, wherein the hair follicle stem cell extraction component is used for quantitatively extracting hair follicle stem cells, and connected with the syringe needle; and the syringe needle is bent and blunt. According to the method and the syringe, hair growth under baldness situation can be improved.

Description

technical field [0001] The invention relates to the field of health care, in particular to a method for culturing hair follicle stem cells and a syringe for treating alopecia. Background technique [0002] Alopecia is formed after abnormal hair loss, which brings inconvenience to patients. Use the hair follicles of the patient's own normal hair, culture, multiply, and purify them in vitro, and then inject them into the patient's bald area to generate new normal hair. At present, stem cells are isolated from umbilical cord blood and injected into bald areas after separation and culture. However, the source of umbilical cord blood directly limits the application of its technology. [0003] In addition, in the process of using umbilical cord blood to separate stem cells, it is necessary to collect umbilical cord blood stem cells with the same or similar histocompatibility genes as the patient, and immune rejection must be considered; the operation process is complicated, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074A61P17/14A61M5/178A61M5/31
Inventor 严其高
Owner 北京雍禾植发技术研究院有限公司
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