Prepn and application of epiderm or hair follicle stem cell from souce of human early embryo

A technology derived from hair follicle stem cells and embryos, applied in the field of stem cells and genetic engineering, can solve the problems of harsh culture conditions and slow proliferation of epidermal stem cells

Inactive Publication Date: 2006-09-20
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
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AI Technical Summary

Benefits of technology

This technology allows us to quickly grow large numbers of hairy cell types from our own body called Eden's Hair Folly MSC(Evans). These small units are similar enough that they have different characteristics such as growth speed or ability to repair damaged tissue. They could potentially help treat medical conditions like burn injuries by providing them back into their original form when needed.

Problems solved by technology

This patents discusses how biological processes involve stimulation between certain types of nerves called neurons, specifically epithelial neural crest cells. While there may exist several ways to improve these processes including improving their effectiveness, reducing damage caused due to external factors like sunlight exposure, heat injury, radiation therapy, chemotherapies, and inflammatory responses. Current techniques include surgery, grafting, plasma donor injection, eletrospun fibre bundles, platelet activation, and histochirculated cell migration.

Method used

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  • Prepn and application of epiderm or hair follicle stem cell from souce of human early embryo
  • Prepn and application of epiderm or hair follicle stem cell from souce of human early embryo
  • Prepn and application of epiderm or hair follicle stem cell from souce of human early embryo

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Embodiment 1

[0025] (1) Preparation of total RNA from human epidermal cells:

[0026] Epidermal cells were obtained from adult human skin through primary culture (Yang Ling, Liu Houqi, etc. Localization, isolation and culture of epidermal stem cells from adult human skin. Journal of Second Military Medical University, 2004, 25(8): 815-817.). The total RNA was obtained by extracting the total RNA by the conventional guanidine isothiocyanate method with the total RNA extraction kit of Shanghai Huashun Bioengineering Co., Ltd.

[0027] (2) Synthesis of primers and construction of pCDNA3.0-EGF eukaryotic expression vector:

[0028] The hEGF gene was transferred to the eukaryotic vector pCDNA3.0. Obtain the cDNA fragment containing the EGF gene according to the literature and recombine it into the eukaryotic expression vector pCDNA3.0 (refer to Chen Tingsu et al., Synthesis and identification of human epidermal growth factor (hEGF) gene and construction of plant expression plasmid, Journal of ...

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Abstract

This invention provides a method for accelerating the proliferation of human mesenchymal stem cells, which comprises the steps of: transfecting recombinant expression carrier containing EGF to human mesenchymal stem cells, and stimulating with a certain concentration of calcium ions during the transfection process to divide into typical stem cells. Under nude mouse subcutaneous microenvironment induction, the stem cells are further divided into skin tissue stem cells, thus directly taking part in the proliferation of nude mouse hair. This invention can obtain rapidly proliferated human mesenchymal stem cells, thus having a potential application in clinical skin wound treatment.

Description

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Claims

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Application Information

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Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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