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Dental pulp stem cell collecting kit

A technology of dental pulp stem cells and kits, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve problems affecting the survival rate of dental pulp stem cells, low expansion multiples of dental pulp stem cells, and reduced cell viability. Achieve the effect of convenient storage and cultivation, increase the freezing time, and increase the amplification factor

Active Publication Date: 2017-12-15
北京唐颐惠康生物医学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to solve this problem, the prior art discloses some methods and kits for the collection, separation and expansion of dental pulp stem cells. The collection methods disclosed in the prior art mostly use tweezers to hold the tooth , this may cause the teeth that have been aseptically treated to be unable to be clamped and fall off during the clamping process, and need to be disinfected again, and the amplification method used in the technology has a low amplification factor for dental pulp stem cells, and the expansion When preserving after augmentation, the preservation solution used affects the survival rate of dental pulp stem cells after resuscitation and reduces the viability of the cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 A dental pulp stem cell collection kit

[0029] The collection set includes a box body 1 and a box cover 2 hinged with the box body 1. The box body 1 is composed of a first box body 11 and a second box body 12 arranged in parallel on the first box body 11; the box cover 2 is hinged. On the second box body 12, the first box body 11 and the second box body 12 are slidingly connected, and the first box body 11 is divided into a plurality of reagent bottle slots 13 by partitions, and reagent bottles are placed in the reagent bottle slots 13 7. Reagent bottle 7 is filled with physiological saline, phosphate buffer saline, tooth cleaning solution, tooth preservation solution, EDTA solution, dental pulp stem cell digestion solution, dental pulp stem cell culture solution and dental pulp stem cell cryopreservation solution; the second box The body 12 is divided into a plurality of accommodating grooves 14 by partitions, and each accommodating groove 14 is used to p...

Embodiment 2

[0030] Example 2 A Dental Pulp Stem Cell Collection Set

[0031] The dental pulp stem cell collection kit provided in Example 2 is different from Example 1 in that the culture composition is mainly prepared from the following ingredients in parts by weight: 2 parts of triethanolamine sulfate, 3 parts of glycine, 0.5 parts of palmitamide, phosphoric acid Choline 0.5 parts.

Embodiment 3

[0032] Example 3 A Dental Pulp Stem Cell Collection Set

[0033] The dental pulp stem cell collection kit provided in Example 3 is different from Example 1 in that the culture composition is mainly prepared from the following ingredients in parts by weight: 3.5 parts of triethanolamine sulfate, 4.5 parts of glycine, 1.2 parts of palmitamide, phosphoric acid Choline 0.8 part.

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Abstract

The invention provides a dental pulp stem cell collecting kit. The dental pulp stem cell collecting kit comprises a box body and a box cover connected with the box body via hinge joint; the box body is composed of a first box body and a second box body; the first box body is divided into a plurality of reagent bottle grooves by separating plates; reagent bottles are placed in the reagent bottle grooves; the second box body is divided into a plurality of storage tanks; and a teeth fixing device, a nerve broach, a scalpel, and a cell filter screen are stored in the storage tanks respectively. The dental pulp stem cell collecting kit can be used for processing of detached teeth conveniently; a culture liquid provided by the dental pulp stem cell collecting kit is capable of increasing amplification times of dental pulp stem cells obviously; a provided stem cell preservation liquid is capable of realizing long term preservation of dental pulp stem cells; and after recovery, the preserved stem cells are still high in activity.

Description

technical field [0001] The invention belongs to the field of collection sets, in particular to a collection set for dental pulp stem cells. Background technique [0002] Stem cells (Stem Cells, SC) are a type of self-renewing pluripotent cells that can differentiate into a variety of functional cells and have the potential to regenerate various tissues and organs and the human body. Stem cells exist in many tissues, can divide into a variety of specialized cells through mitosis and differentiation, and can use self-renewal to provide more stem cells. Stem cells come from many sources, including bone marrow, umbilical cord, teeth, etc. [0003] Dental pulp stem cells (dental pulp stem cells, DPSCs) were first discovered in 2000, which can exist in deciduous teeth and wisdom teeth in vitro. This is a type of cell with high proliferative ability, high self-renewal ability, and multiple differentiation abilities. Studies have used the cells to successfully construct dental pu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12M1/26A01N1/02
CPCA01N1/0221C12M33/14C12N5/0664
Inventor 刘俊江曹毓琳时兆田白志惠王俊王沾
Owner 北京唐颐惠康生物医学技术有限公司
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