Stem cell exosome preparation as well as preparation method and application thereof

An exosome and stem cell technology, applied in the biological field, can solve the problems of general treatment effect, inability to fundamentally cure the disease, and large patient trauma, and achieve the effects of good curative effect, shortened time, and convenient preparation of preparations.

Inactive Publication Date: 2017-03-29
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Nephritis is usually treated with drugs or surgery, the therapeutic effect is average, and the trauma to the patient is relatively large
Moreover, the current treatment methods can only relieve nephritis, but cannot fundamentally cure the disease.

Method used

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  • Stem cell exosome preparation as well as preparation method and application thereof
  • Stem cell exosome preparation as well as preparation method and application thereof
  • Stem cell exosome preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of Dental Pulp Stem Cell Exosomes

[0046] Primary culture of dental pulp stem cells: Take the wisdom teeth of healthy people and perform aseptic cleaning in an ultra-clean bench. After cleaning, cut the teeth with forceps and take out the pulp; cut the pulp with surgical scissors, and use type I collagenase Digest with neutral protease for 30min, centrifuge at 1500rpm for 10min, discard the supernatant, resuspend with DMEM+10%FBS complete medium, inoculate into 6-well plate, transfer to 5%CO 2, Cultivate in an environment of 37°C; discard the supernatant after 5 days of culture, supplement with new complete medium and continue to culture, and the cells can be passaged when they form larger clones;

[0047] Subculture of dental pulp stem cells: Take subcultured P0 cells, discard the original culture medium in the dish, add 10mL PBS buffer solution to gently wash the cell growth surface; discard the PBS washing solution, add 2mL 0.25% trypsin, and s...

Embodiment 2

[0053] Example 2 Preparation of exosome preparation

[0054] Add human serum albumin to normal saline to prepare a human serum albumin solution with a mass concentration of human serum albumin of 5%; then add the dental pulp stem cell exosomes and dental pulp prepared in Example 1 to the human serum albumin solution Stem cells, so that each 5mL solution contains 5×10 6 dental pulp stem cells and from 5×10 6 The exosomes extracted from dental pulp stem cells were prepared and stored at 4°C.

Embodiment 3

[0055] Example 3 Preparation of exosome preparation

[0056] Add human serum albumin to normal saline to prepare a human serum albumin solution with a mass concentration of human serum albumin of 2%; then add the dental pulp stem cell exosomes prepared in Example 1 and Dental pulp stem cells, so that each 5mL solution contains 5×10 6 dental pulp stem cells and from 1×10 6 The exosomes extracted from dental pulp stem cells were prepared and stored at 4°C.

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Abstract

The invention relates to the field of bio-technology, and discloses a stem cell exosome preparation as well as a preparation method and an application thereof. The stem cell exosome preparation provided by the invention consists of a dental pulp stem cell exosome, dental pulp stem cells, human albumin and a solvent, wherein the dental pulp stem cell exosome is extracted from the dental pulp stem cells; every 5ml of the preparation contains 1*10<6>-1*10<7> pieces of the exosome extracted from the dental pulp stem cells; the content of the dental pulp stem cells is 5*10<6> cells / 5mL; the mass concentration of the human albumin is 2-8%; and the solvent is normal saline. The invention also discloses the preparation method of the exosome preparation and a preparation method of the dental pulp stem cell exosome. The invention also discloses an application of the exosome preparation or the exosome preparation prepared by the preparation method in preparing biological preparations for treating nephritis. The stem cell exosome preparation provided by the invention has a good curative effect on the nephritis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a stem cell exosome preparation and its preparation method and application. Background technique [0002] Exosomes are nanoscale membranous vesicles that are released into the extracellular environment by the fusion of multivesicular bodies (MVBs) and cell membranes of eukaryotic cells. Secretion, which contains different types of proteins, lipids, mRNAs, microRNAs, signaling molecules and other biologically active substances, which are easier to fuse with the cell membranes of adjacent cells and selectively deliver biologically active substances to recipient cells , transmit information between different cells, regulate signal transduction between cells, and exert various biological functions. [0003] The physiological function of the kidney is mainly to excrete metabolites, regulate water, electrolyte and acid-base balance, secrete a variety of active substances, maintain the sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K47/42C12N5/074A61P13/12
CPCA61K35/28A61K47/42C12N5/0664C12N2509/00A61K2300/00
Inventor 陈海佳葛啸虎王一飞卢瑞珊王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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