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Stem cell exosome preparation, and preparation method and application thereof

A technology of exosomes and stem cells, applied in the direction of medical formulas, medical preparations containing active ingredients, digestive system, etc., can solve problems such as inability to fundamentally cure diseases, mediocre therapeutic effects, and large trauma to patients, and achieve the goal of treating gastritis Effects of disease, good curative effect, and ease of preparation preparation

Inactive Publication Date: 2017-03-15
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Drugs or surgery are commonly used to treat gastritis, the therapeutic effect is average, and the trauma to the patient is relatively large
Moreover, the current treatment methods can only relieve gastritis, but cannot fundamentally cure the disease.

Method used

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  • Stem cell exosome preparation, and preparation method and application thereof
  • Stem cell exosome preparation, and preparation method and application thereof
  • Stem cell exosome preparation, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049]Example 1 Preparation of Dental Pulp Stem Cell Exosomes

[0050] Primary culture of dental pulp stem cells: Take the wisdom teeth of healthy people and perform aseptic cleaning in an ultra-clean bench. After cleaning, cut the teeth with forceps and take out the pulp; cut the pulp with surgical scissors, and use type I collagenase Digest with neutral protease for 30min, centrifuge at 1500rpm for 10min, discard the supernatant, resuspend with DMEM+10%FBS complete medium, inoculate into 6-well plate, transfer to 5%CO 2 , cultured at 37°C; after 5 days of culture, the supernatant was discarded, and new complete medium was added to continue the culture. After the cells formed larger clones, they could be passaged; see the microscopic image of the primary cells. figure 2 ;

[0051] Subculture of dental pulp stem cells: Take subcultured P0 cells, discard the original culture medium in the dish, add 10mL PBS buffer solution to gently wash the cell growth surface; discard the P...

Embodiment 2

[0057] Example 2 Preparation of exosome preparation

[0058] Add bFGF to normal saline to make a normal saline solution with a bFGF content of 10 ng / mL; then add the dental pulp stem cell exosomes and dental pulp stem cells prepared in Example 1 to the solution, so that every 200 μL of normal saline solution contains 2 ×10 6 dental pulp stem cells and from 5×10 6 The exosomes extracted from dental pulp stem cells were prepared and stored at 4°C.

Embodiment 3

[0059] Example 3 Preparation of exosome preparation

[0060] Add bFGF to normal saline to prepare a normal saline solution with a bFGF content of 5 ng / mL; then add the dental pulp stem cell exosomes and dental pulp stem cells prepared in Example 1 to the solution, so that every 200 μL of normal saline solution contains 1 ×10 6 dental pulp stem cells and from 1×10 6 The exosomes extracted from dental pulp stem cells were prepared and stored at 4°C.

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Abstract

The invention relates to the field of biotechnology and discloses a stem cell exosome preparation, and a preparation method and application thereof. The stem cell exosome preparation disclosed by the invention comprises dental pulp stem cell exosomes, dental pulp stem cells, basic fibroblast growth factors and a solvent, wherein the dental pulp stem cell exosomes are exosomes extracted from the dental pulp stem cells; 200 microliters of the preparation contain the exosomes extracted from 1*10<6> to 1*10<7> dental pulp stem cells and 1*10<6> to 3*10<6> dental pulp stem cells; the content of the basic fibroblast growth factors is 5 to 15 ng / mL; and the solvent is normal saline. The invention also discloses the exosome preparation or application of the exosome preparation prepared by the preparation method to preparation of a biological preparation for treating gastritis. The stem cell exosome preparation disclosed by the invention has a good curative effect on gastritis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a stem cell exosome preparation and its preparation method and application. Background technique [0002] Exosomes are nanoscale membranous vesicles that are released into the extracellular environment by the fusion of multivesicular bodies (MVBs) and cell membranes of eukaryotic cells. Secretion, which contains different types of proteins, lipids, mRNAs, microRNAs, signaling molecules and other biologically active substances, which are easier to fuse with the cell membranes of adjacent cells and selectively deliver biologically active substances to recipient cells , transmit information between different cells, regulate signal transduction between cells, and exert various biological functions. [0003] Gastritis is an acute and chronic inflammation of the gastric mucosa caused by a variety of different etiologies, often accompanied by epithelial injury, mucosal inflammatory respons...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61P1/04A61K35/28
CPCA61K35/28A61K38/1825A61K2300/00
Inventor 陈海佳葛啸虎王一飞卢瑞珊王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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